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Article Open Access

Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells

  • Authors:
    • Peng Wu
    • Peng Zhang
    • Hanjiang Zheng
    • Baoqi Zuo
    • Xiaofeng Duan
    • Junjun Chen
    • Xinhong Wang
    • Yixin Shen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Orthopedics, The Second Hospital of Jingzhou, Jingzhou, Hubei 434000, P.R. China, National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215004, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 123-130
    |
    Published online on: November 6, 2018
       https://doi.org/10.3892/etm.2018.6933
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Abstract

Transplantation of olfactory ensheathing cells (OECs) has potential for treating spinal cord and brain injury. However, they are void of an extracellular matrix to support cell growth and migration. Engineering of tissue to mimic the extracellular matrix is a potential solution for neural repair. Tussah silk fibroin (TSF) has good biocompatibility and an Arg‑Gly‑Asp tripeptide sequence. A small number of studies have assessed the effect of the diameter of TSF nanofibers on cell adhesion, growth and migration. In the present study, TSF nanofibers with a diameter of 400 and 1,200 nm were prepared using electrospinning technology; these were then used as scaffolds for OECs. The structure and morphology of the TSF nanofibers were characterized by scanning electron microscopy (SEM) and Fourier‑transform infrared spectroscopy. An inverted‑phase contrast microscope and SEM were used to observe the morphology of OECs on the TSF nanofibers. The effect on the adhesion of the cells was observed following crystal violet staining. The phenotype of the cells and the maximum axon length on the scaffolds were evaluated by immunostaining for nerve growth factor receptor p75. Cell proliferation and viability were assessed by an MTT assay and a Live/Dead reagent kit. The migration efficiency of OECs was observed using live‑cell microscopy. The results indicated that a 400‑nm TSF fiber scaffold was more conducive to OEC adhesion, growth and migration compared with a 1,200‑nm TSF scaffold. The phenotype of the OECs was normal, and no difference in OEC phenotype was observe when comparing those on TSF nanofibers to those on PLL. The present study may provide guidance regarding the preparation of tissue‑engineered materials for neural repair.
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Copy and paste a formatted citation
Spandidos Publications style
Wu P, Zhang P, Zheng H, Zuo B, Duan X, Chen J, Wang X and Shen Y: Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells. Exp Ther Med 17: 123-130, 2019.
APA
Wu, P., Zhang, P., Zheng, H., Zuo, B., Duan, X., Chen, J. ... Shen, Y. (2019). Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells. Experimental and Therapeutic Medicine, 17, 123-130. https://doi.org/10.3892/etm.2018.6933
MLA
Wu, P., Zhang, P., Zheng, H., Zuo, B., Duan, X., Chen, J., Wang, X., Shen, Y."Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells". Experimental and Therapeutic Medicine 17.1 (2019): 123-130.
Chicago
Wu, P., Zhang, P., Zheng, H., Zuo, B., Duan, X., Chen, J., Wang, X., Shen, Y."Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells". Experimental and Therapeutic Medicine 17, no. 1 (2019): 123-130. https://doi.org/10.3892/etm.2018.6933
Copy and paste a formatted citation
x
Spandidos Publications style
Wu P, Zhang P, Zheng H, Zuo B, Duan X, Chen J, Wang X and Shen Y: Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells. Exp Ther Med 17: 123-130, 2019.
APA
Wu, P., Zhang, P., Zheng, H., Zuo, B., Duan, X., Chen, J. ... Shen, Y. (2019). Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells. Experimental and Therapeutic Medicine, 17, 123-130. https://doi.org/10.3892/etm.2018.6933
MLA
Wu, P., Zhang, P., Zheng, H., Zuo, B., Duan, X., Chen, J., Wang, X., Shen, Y."Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells". Experimental and Therapeutic Medicine 17.1 (2019): 123-130.
Chicago
Wu, P., Zhang, P., Zheng, H., Zuo, B., Duan, X., Chen, J., Wang, X., Shen, Y."Biological effects different diameters of Tussah silk fibroin nanofibers on olfactory ensheathing cells". Experimental and Therapeutic Medicine 17, no. 1 (2019): 123-130. https://doi.org/10.3892/etm.2018.6933
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