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PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells

  • Authors:
    • Haiyan Jia
    • Zhen Guo
    • Yanfen Yao
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care Medicine, Shandong Provincial Third Hospital, Jinan, Shandong 250031, P.R. China, Department of Urology Surgery, Qianfoshan Hospital of Shandong Province, Jinan, Shandong 250014, P.R. China
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 199-204
    |
    Published online on: November 9, 2018
       https://doi.org/10.3892/etm.2018.6951
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Abstract

Protective effect of phosphodiesterase 5 (PDE5) inhibitor sildenafil on hypoxic injury of isolated myocardial cells and its mechanism of action were investigated. Myocardial cells of neonatal mice were isolated, cultured and divided into blank, control, and PDE5 inhibitor group. Cells in the control and the PDE5 inhibitor group were treated with hypoxia and serum deprivation for 6 h to simulate the myocardial ischemia process in vivo, while those in the PDE5 inhibitor group were treated with 1 µmol/l sildenafil. The cell viability was detected via Cell Counting kit-8 (CCK-8), the cytotoxicity was detected via lactate dehydrogenase release assay, and the apoptosis level was detected via flow cytometry, Hoechst staining and caspase-3 activity assay. Moreover, changes in mitochondrial membrane potential of myocardial cells were detected via JC-1 staining and flow cytometry, fluorescein adenosine triphosphate (ATP) assay kit was used to detect the production of ATP in myocardial cells, and reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the level of Sirt3 messenger ribonucleic acid (mRNA) in myocardial cells. Finally, the expression and changes of Sirt3, peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) and acetylated PGC-1α were detected via western blot analysis. After hypoxia treatment, the cell viability was decreased, the cytotoxic effect was enhanced, the percentage of apoptosis was increased, the activity of apoptosis-related protein was increased, the mitochondrial membrane potential was decreased, the production of ATP was reduced, the expression of Sirt3 was decreased, and the acetylation level of PGC-1α was increased. However, after pretreatment with sildenafil, the damage to membrane potential of myocardial cells was significantly alleviated, and the production of ATP was increased. At the same time, myocardial cell apoptosis was decreased, Sirt3 expression was increased and PGC-1α acetylation was decreased. PDE5 inhibitor inhibits apoptosis of hypoxic myocardial cells through protecting mitochondrial function.
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1 

Nanchen D, Leening MJ, Locatelli I, Cornuz J, Kors JA, Heeringa J, Deckers JW, Hofman A, Franco OH, Stricker BH, et al: Resting heart rate and the risk of heart failure in healthy adults: The Rotterdam Study. Circ Heart Fail. 6:403–410. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Cowie MR, Wood DA, Coats AJ, Thompson SG, Suresh V, Poole-Wilson PA and Sutton GC: Survival of patients with a new diagnosis of heart failure: A population based study. Heart. 83:505–510. 2000. View Article : Google Scholar : PubMed/NCBI

3 

van Bilsen M, Smeets PJ, Gilde AJ and van der Vusse GJ: Metabolic remodelling of the failing heart: The cardiac burn-out syndrome? Cardiovasc Res. 61:218–226. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Liu YF, Chu YY, Zhang XZ, Zhang M, Xie FG, Zhou M, Wen HH and Shu AH: TGFβ1 protects myocardium from apoptosis and oxidative damage after ischemia reperfusion. Eur Rev Med Pharmacol Sci. 21:1551–1558. 2017.PubMed/NCBI

5 

Lin J, Handschin C and Spiegelman BM: Metabolic control through the PGC-1 family of transcription coactivators. Cell Metab. 1:361–370. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Huss JM, Torra IP, Staels B, Giguère V and Kelly DP: Estrogen-related receptor alpha directs peroxisome proliferator-activated receptor alpha signaling in the transcriptional control of energy metabolism in cardiac and skeletal muscle. Mol Cell Biol. 24:9079–9091. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Li X, Zhang S, Blander G, Tse JG, Krieger M and Guarente L: SIRT1 deacetylates and positively regulates the nuclear receptor LXR. Mol Cell. 28:91–106. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Sundaresan NR, Gupta M, Kim G, Rajamohan SB, Isbatan A and Gupta MP: Sirt3 blocks the cardiac hypertrophic response by augmenting Foxo3a-dependent antioxidant defense mechanisms in mice. J Clin Invest. 119:2758–2771. 2009.PubMed/NCBI

9 

Kukreja RC, Salloum FN, Das A, Koka S, Ockaili RA and Xi L: Emerging new uses of phosphodiesterase-5 inhibitors in cardiovascular diseases. Exp Clin Cardiol. 16:e30–e35. 2011.PubMed/NCBI

10 

Das A, Xi L and Kukreja RC: Protein kinase G-dependent cardioprotective mechanism of phosphodiesterase-5 inhibition involves phosphorylation of ERK and GSK3beta. J Biol Chem. 283:29572–29585. 2008. View Article : Google Scholar : PubMed/NCBI

11 

De Toni L, Strapazzon G, Gianesello L, Caretta N, Pilon C, Bruttocao A and Foresta C: Effects of type 5-phosphodiesterase inhibition on energy metabolism and mitochondrial biogenesis in human adipose tissue ex vivo. J Endocrinol Invest. 34:738–741. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Ventura-Clapier R, Garnier A and Veksler V: Transcriptional control of mitochondrial biogenesis: The central role of PGC-1alpha. Cardiovasc Res. 79:208–217. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Bellizzi D, Rose G, Cavalcante P, Covello G, Dato S, De Rango F, Greco V, Maggiolini M, Feraco E, Mari V, et al: A novel VNTR enhancer within the SIRT3 gene, a human homologue of SIR2, is associated with survival at oldest ages. Genomics. 85:258–263. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Lu Z, Xu X, Hu X, Lee S, Traverse JH, Zhu G, Fassett J, Tao Y, Zhang P, dos Remedios C, et al: Oxidative stress regulates left ventricular PDE5 expression in the failing heart. Circulation. 121:1474–1483. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Das A, Durrant D, Salloum FN, Xi L and Kukreja RC: PDE5 inhibitors as therapeutics for heart disease, diabetes and cancer. Pharmacol Ther. 147:12–21. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jia H, Guo Z and Yao Y: PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells. Exp Ther Med 17: 199-204, 2019.
APA
Jia, H., Guo, Z., & Yao, Y. (2019). PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells. Experimental and Therapeutic Medicine, 17, 199-204. https://doi.org/10.3892/etm.2018.6951
MLA
Jia, H., Guo, Z., Yao, Y."PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells". Experimental and Therapeutic Medicine 17.1 (2019): 199-204.
Chicago
Jia, H., Guo, Z., Yao, Y."PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells". Experimental and Therapeutic Medicine 17, no. 1 (2019): 199-204. https://doi.org/10.3892/etm.2018.6951
Copy and paste a formatted citation
x
Spandidos Publications style
Jia H, Guo Z and Yao Y: PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells. Exp Ther Med 17: 199-204, 2019.
APA
Jia, H., Guo, Z., & Yao, Y. (2019). PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells. Experimental and Therapeutic Medicine, 17, 199-204. https://doi.org/10.3892/etm.2018.6951
MLA
Jia, H., Guo, Z., Yao, Y."PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells". Experimental and Therapeutic Medicine 17.1 (2019): 199-204.
Chicago
Jia, H., Guo, Z., Yao, Y."PDE5 inhibitor protects the mitochondrial function of hypoxic myocardial cells". Experimental and Therapeutic Medicine 17, no. 1 (2019): 199-204. https://doi.org/10.3892/etm.2018.6951
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