Open Access

miRNA-101 promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells

  • Authors:
    • Feng Gao
    • Chuangang Peng
    • Changjun Zheng
    • Shanyong Zhang
    • Minfei Wu
  • View Affiliations

  • Published online on: November 12, 2018     https://doi.org/10.3892/etm.2018.6959
  • Pages: 175-180
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effect and related mechanisms of miR-101 on the chondrogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs) were investigated. The expression level of miR-101 was detected during chondrogenic differentiation. Three groups were established to study the potential function between miR-101 and chondrogenic differentiation: miR-NC group (negative control), miR-101 mimics (BMSCs transfected by miR-101 mimics) and mimics + inhibitor (BMSCs transfected by miR-101 mimics and inhibitor), after the induction of chondrogenic differentiation, the cell viability of MSCs and chondrogenic markers were determined, further, the expression level of Sox9 and Runx2 were detected. In our present research, miR-101 was found upregulated during chondrogenic differentiation of MSCs. Compared with the miR-NC group, the cell viability of MSCs was enhanced and the expression level of chondrogenic markers were respectively gained. The expression level of Sox9 was increased but the expression level of Runx2 was decreased by treatment of miR-101 mimics after induction of chondrogenic differentiation. However, these variations of the indicators were reversed by the intervention using the miR-101 inhibitor. Collectively, our research revealed promotion function of miR-101 on chondrogenic differentiation of MSCs, indicating that miR-101 could be a potential therapeutic strategy for the treatment of osteoarthritis (OA).
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao F, Peng C, Zheng C, Zhang S and Wu M: miRNA-101 promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells. Exp Ther Med 17: 175-180, 2019
APA
Gao, F., Peng, C., Zheng, C., Zhang, S., & Wu, M. (2019). miRNA-101 promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells. Experimental and Therapeutic Medicine, 17, 175-180. https://doi.org/10.3892/etm.2018.6959
MLA
Gao, F., Peng, C., Zheng, C., Zhang, S., Wu, M."miRNA-101 promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells". Experimental and Therapeutic Medicine 17.1 (2019): 175-180.
Chicago
Gao, F., Peng, C., Zheng, C., Zhang, S., Wu, M."miRNA-101 promotes chondrogenic differentiation in rat bone marrow mesenchymal stem cells". Experimental and Therapeutic Medicine 17, no. 1 (2019): 175-180. https://doi.org/10.3892/etm.2018.6959