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In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review)

  • Authors:
    • Mihaela Adriana Ilie
    • Constantin Caruntu
    • Daniela Lixandru
    • Mircea Tampa
    • Simona‑Roxana Georgescu
    • Maria‑Magdalena Constantin
    • Carolina Constantin
    • Monica Neagu
    • Sabina Andrada Zurac
    • Daniel Boda
  • View Affiliations / Copyright

    Affiliations: Dermatology Research Laboratory, ‘Carol Davila’ University of Medicine and Pharmacy, 050474 Bucharest, Romania, Department of Physiology, ‘Carol Davila’ University of Medicine and Pharmacy, 050474 Bucharest, Romania, Department of Biochemistry, ‘Carol Davila’ University of Medicine and Pharmacy, 050474 Bucharest, Romania, Department of Dermatology, ‘Victor Babes’ Hospital, ‘Carol Davila’ University of Medicine and Pharmacy, 030303 Bucharest, Romania, The 2nd Department of Dermatology, Colentina Clinical Hospital, 020125 Bucharest, Romania, Department of Immunology, ‘Victor Babes’ National Institute of Pathology, 050096 Bucharest, Romania, Department of Pathology, Colentina Clinical Hospital, 020125 Bucharest, Romania
    Copyright: © Ilie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1004-1011
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    Published online on: November 16, 2018
       https://doi.org/10.3892/etm.2018.6981
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Abstract

In vivo confocal laser scanning microscopy (CLSM) is a novel imaging technique that provides noninvasive, morphological characterization of skin structures with a resolution that is very close to that of light microscopy. Moreover, as it allows repeated imaging of the same skin area at different time-points, it is an excellent method for monitoring disease course, response to treatment or specific stimuli and a path to study dynamic phenomena in real‑time. To date, two different variants of in vivo CLSM have been authorized in dermatological field, namely the reflectance confocal microscopy predominantly for clinical diagnosis and the fluorescence confocal microscopy mainly for research purposes. This study describes the principles of in vivo CLSM technique, its role in the diagnosis and monitoring of inflammatory skin diseases, as well as some promising research directions to study the dynamics of skin inflammation using this method. In vivo CLSM evaluation of inflammatory dermatoses and of the skin inflammatory component in various diseases has an undoubted potential with broad applications ranging from clinical, morphological to experimental, functional studies involving the skin.
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Copy and paste a formatted citation
Spandidos Publications style
Ilie MA, Caruntu C, Lixandru D, Tampa M, Georgescu SR, Constantin MM, Constantin C, Neagu M, Zurac SA, Boda D, Boda D, et al: In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review). Exp Ther Med 17: 1004-1011, 2019.
APA
Ilie, M.A., Caruntu, C., Lixandru, D., Tampa, M., Georgescu, S., Constantin, M. ... Boda, D. (2019). In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review). Experimental and Therapeutic Medicine, 17, 1004-1011. https://doi.org/10.3892/etm.2018.6981
MLA
Ilie, M. A., Caruntu, C., Lixandru, D., Tampa, M., Georgescu, S., Constantin, M., Constantin, C., Neagu, M., Zurac, S. A., Boda, D."In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review)". Experimental and Therapeutic Medicine 17.2 (2019): 1004-1011.
Chicago
Ilie, M. A., Caruntu, C., Lixandru, D., Tampa, M., Georgescu, S., Constantin, M., Constantin, C., Neagu, M., Zurac, S. A., Boda, D."In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review)". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1004-1011. https://doi.org/10.3892/etm.2018.6981
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Spandidos Publications style
Ilie MA, Caruntu C, Lixandru D, Tampa M, Georgescu SR, Constantin MM, Constantin C, Neagu M, Zurac SA, Boda D, Boda D, et al: In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review). Exp Ther Med 17: 1004-1011, 2019.
APA
Ilie, M.A., Caruntu, C., Lixandru, D., Tampa, M., Georgescu, S., Constantin, M. ... Boda, D. (2019). In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review). Experimental and Therapeutic Medicine, 17, 1004-1011. https://doi.org/10.3892/etm.2018.6981
MLA
Ilie, M. A., Caruntu, C., Lixandru, D., Tampa, M., Georgescu, S., Constantin, M., Constantin, C., Neagu, M., Zurac, S. A., Boda, D."In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review)". Experimental and Therapeutic Medicine 17.2 (2019): 1004-1011.
Chicago
Ilie, M. A., Caruntu, C., Lixandru, D., Tampa, M., Georgescu, S., Constantin, M., Constantin, C., Neagu, M., Zurac, S. A., Boda, D."In vivo confocal laser scanning microscopy imaging of skin inflammation: Clinical applications and research directions (Review)". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1004-1011. https://doi.org/10.3892/etm.2018.6981
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