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Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis

  • Authors:
    • Shuang Pang
    • Jing Lv
    • Shengzhi Wang
    • Guanqi Yang
    • Xiaohuan Ding
    • Jun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, P.R. China, Department of Pediatrics, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, P.R. China
    Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 621-632
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    Published online on: November 30, 2018
       https://doi.org/10.3892/etm.2018.7038
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Abstract

Long non‑coding RNAs (lncRNAs) serve an essential role in regulating immunological functions. However, their impact on Henoch‑Schönlein purpura nephritis (HSPN), has remained elusive. The present study determined the expression of lncRNAs and mRNAs in the peripheral blood of 6 children with HSPN and recruited 4 healthy children for comparative study. High‑throughput sequencing revealed outstanding differences in lncRNA and mRNA expression, which were verified through reverse transcription‑quantitative polymerase chain reaction. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to investigate the associated biological functions and possible mechanisms of lncRNAs and mRNAs in HSPN. A total of 820 differentially expressed lncRNAs between the two groups were identified, of which 34 were upregulated and 786 were downregulated. Simultaneously, a total of 3,557 mRNAs were also identified to be differentially expressed, of which 1,232 were upregulated and 2,325 were downregulated. The results revealed that the expression of lncRNAs including ENST00000378432, ENST00000571370, uc001kfc.1 and uc010qna.2 was decreased in HSPN patients compared with that in healthy controls. These lncRNAs were associated with the p53 signaling pathway and apoptosis‑associated genes (AKT2, tumor protein 53, phosphatase and tensin homolog and FAS). Further studies of those lncRNAs will be performed to elucidate their functions in apoptosis. Complete raw data files were deposited in the Gene Expression Omnibus (GEO) at National Center for Biotechnology information under the GEO accession no. GSE102114 (www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102114).
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Copy and paste a formatted citation
Spandidos Publications style
Pang S, Lv J, Wang S, Yang G, Ding X and Zhang J: Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis. Exp Ther Med 17: 621-632, 2019.
APA
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., & Zhang, J. (2019). Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis. Experimental and Therapeutic Medicine, 17, 621-632. https://doi.org/10.3892/etm.2018.7038
MLA
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., Zhang, J."Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis". Experimental and Therapeutic Medicine 17.1 (2019): 621-632.
Chicago
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., Zhang, J."Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis". Experimental and Therapeutic Medicine 17, no. 1 (2019): 621-632. https://doi.org/10.3892/etm.2018.7038
Copy and paste a formatted citation
x
Spandidos Publications style
Pang S, Lv J, Wang S, Yang G, Ding X and Zhang J: Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis. Exp Ther Med 17: 621-632, 2019.
APA
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., & Zhang, J. (2019). Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis. Experimental and Therapeutic Medicine, 17, 621-632. https://doi.org/10.3892/etm.2018.7038
MLA
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., Zhang, J."Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis". Experimental and Therapeutic Medicine 17.1 (2019): 621-632.
Chicago
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., Zhang, J."Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis". Experimental and Therapeutic Medicine 17, no. 1 (2019): 621-632. https://doi.org/10.3892/etm.2018.7038
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