Open Access

Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis

  • Authors:
    • Shuang Pang
    • Jing Lv
    • Shengzhi Wang
    • Guanqi Yang
    • Xiaohuan Ding
    • Jun Zhang
  • View Affiliations

  • Published online on: November 30, 2018     https://doi.org/10.3892/etm.2018.7038
  • Pages: 621-632
  • Copyright: © Pang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) serve an essential role in regulating immunological functions. However, their impact on Henoch‑Schönlein purpura nephritis (HSPN), has remained elusive. The present study determined the expression of lncRNAs and mRNAs in the peripheral blood of 6 children with HSPN and recruited 4 healthy children for comparative study. High‑throughput sequencing revealed outstanding differences in lncRNA and mRNA expression, which were verified through reverse transcription‑quantitative polymerase chain reaction. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to investigate the associated biological functions and possible mechanisms of lncRNAs and mRNAs in HSPN. A total of 820 differentially expressed lncRNAs between the two groups were identified, of which 34 were upregulated and 786 were downregulated. Simultaneously, a total of 3,557 mRNAs were also identified to be differentially expressed, of which 1,232 were upregulated and 2,325 were downregulated. The results revealed that the expression of lncRNAs including ENST00000378432, ENST00000571370, uc001kfc.1 and uc010qna.2 was decreased in HSPN patients compared with that in healthy controls. These lncRNAs were associated with the p53 signaling pathway and apoptosis‑associated genes (AKT2, tumor protein 53, phosphatase and tensin homolog and FAS). Further studies of those lncRNAs will be performed to elucidate their functions in apoptosis. Complete raw data files were deposited in the Gene Expression Omnibus (GEO) at National Center for Biotechnology information under the GEO accession no. GSE102114 (www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102114).
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pang S, Lv J, Wang S, Yang G, Ding X and Zhang J: Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis. Exp Ther Med 17: 621-632, 2019
APA
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., & Zhang, J. (2019). Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis. Experimental and Therapeutic Medicine, 17, 621-632. https://doi.org/10.3892/etm.2018.7038
MLA
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., Zhang, J."Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis". Experimental and Therapeutic Medicine 17.1 (2019): 621-632.
Chicago
Pang, S., Lv, J., Wang, S., Yang, G., Ding, X., Zhang, J."Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Schönlein purpura nephritis". Experimental and Therapeutic Medicine 17, no. 1 (2019): 621-632. https://doi.org/10.3892/etm.2018.7038