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Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration

  • Authors:
    • Mihai Bucur
    • Carolina Constantin
    • Monica Neagu
    • Sabina Zurac
    • Octavian Dinca
    • Cristian Vladan
    • Mirela Cioplea
    • Cristiana Popp
    • Luciana Nichita
    • Ecaterina Ionescu
  • View Affiliations / Copyright

    Affiliations: Faculty of Dental Medicine, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania, Department of Pathology, Colentina University Hospital, 020125 Bucharest, Romania
    Copyright: © Bucur et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 982-989
    |
    Published online on: December 6, 2018
       https://doi.org/10.3892/etm.2018.7063
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Abstract

Wound healing process comprises a complex network of cells and molecules that are regulated in order to pursue tissue regeneration. Our study focused on the capacity of alveolar blood clots (ABCs), platelet-rich fibrin (PRF) and plasma rich in growth factors (PRGF) to induce in vitro fibroblasts proliferation and migration as a measure of alveolar regeneration. Using cellular impedance with xCELLigence technology we quantified the proliferation and the migration capacity of L929 fibroblast standard cell line in the presence of 4 different ABCs and 3 different PRFs harvested from healthy individuals during standard tooth extraction. We obtained a clear cellular proliferation induced by the compounds mainly after 24 h of cultivation, in a dose-dependent manner. After 48 h of cultivation we registered activated proliferation, but slightly decreased compared to the 24 h profile. Our data confirm that the presence of the blood clot is involved in the regenerative processes. The migratory capacity of fibroblasts was statistically activated by the PL compounds while not affected by the tested PRFs. The chemical mediators present within the blood clot, either produced by inflammatory cells captive within, or by endothelial or mesenchymal cells induced fibroblastic proliferation and subsequent collagen deposition.
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Copy and paste a formatted citation
Spandidos Publications style
Bucur M, Constantin C, Neagu M, Zurac S, Dinca O, Vladan C, Cioplea M, Popp C, Nichita L, Ionescu E, Ionescu E, et al: Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration. Exp Ther Med 17: 982-989, 2019.
APA
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C. ... Ionescu, E. (2019). Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration. Experimental and Therapeutic Medicine, 17, 982-989. https://doi.org/10.3892/etm.2018.7063
MLA
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C., Cioplea, M., Popp, C., Nichita, L., Ionescu, E."Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration". Experimental and Therapeutic Medicine 17.2 (2019): 982-989.
Chicago
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C., Cioplea, M., Popp, C., Nichita, L., Ionescu, E."Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration". Experimental and Therapeutic Medicine 17, no. 2 (2019): 982-989. https://doi.org/10.3892/etm.2018.7063
Copy and paste a formatted citation
x
Spandidos Publications style
Bucur M, Constantin C, Neagu M, Zurac S, Dinca O, Vladan C, Cioplea M, Popp C, Nichita L, Ionescu E, Ionescu E, et al: Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration. Exp Ther Med 17: 982-989, 2019.
APA
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C. ... Ionescu, E. (2019). Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration. Experimental and Therapeutic Medicine, 17, 982-989. https://doi.org/10.3892/etm.2018.7063
MLA
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C., Cioplea, M., Popp, C., Nichita, L., Ionescu, E."Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration". Experimental and Therapeutic Medicine 17.2 (2019): 982-989.
Chicago
Bucur, M., Constantin, C., Neagu, M., Zurac, S., Dinca, O., Vladan, C., Cioplea, M., Popp, C., Nichita, L., Ionescu, E."Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration". Experimental and Therapeutic Medicine 17, no. 2 (2019): 982-989. https://doi.org/10.3892/etm.2018.7063
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