Anti‑inflammatory effect of stromal vascular fraction cells in fat transplantation

Zhu,Ming; Xue,Jian; Lu,Shiliang; Yuan,Yi; Liao,Yunjun; Qiu,Jingjing; Liu,Cheng; Liao,Qingting

doi:10.3892/etm.2018.7082

February-2019 Volume 17 Issue 2

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February-2019 Volume 17 Issue 2

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Article

Anti‑inflammatory effect of stromal vascular fraction cells in fat transplantation

Authors:
- Ming Zhu
- Jian Xue
- Shiliang Lu
- Yi Yuan
- Yunjun Liao
- Jingjing Qiu
- Cheng Liu
- Qingting Liao
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Affiliations: Department of Dermatology, Guangdong Provincial Corps Hospital of Chinese People's Armed Police Forces, Guangzhou Medical University, Guangzhou, Guangdong 510000, P.R. China, Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Pages: 1435-1439
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Published online on: December 11, 2018

https://doi.org/10.3892/etm.2018.7082
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Abstract

In previous studies, it has been demonstrated that the stromal vascular fraction (SVF) promoted the retention rate of grafts following fat transplantation through its angiogenic properties. However, the effect of SVF on inflammatory response subsequent to fat transplantation remains unclear. Fat from C57 mice were mixed with green fluorescent protein (GFP) SVF cells or phosphate‑buffered saline and transplanted into C57BL/6 mice. The graft was harvested and immunofluorescent staining was performed on 1, 7, 14 and 30 days. The numbers of vessels and macrophages, as well as the inflammation level, in the two groups were evaluated. Although the GFP fluorescence indicated that most SVF cells were dead following transplantation, there was a higher graft retention rate in the SVF‑assisted group compared with the control group. The SVF‑assisted group also presented higher expression levels of interleukin (IL)‑6 and tumor necrosis factor‑α, and lower levels of IL‑10, as well as increased M2 macrophages in comparison with the control group. Therefore, the results indicated that SVF promoted the retention rate of grafts following fat transplantation through the well‑known pro‑angiogenic mechanism (paracrine function and involvement in the formation of new vessels), as well as through the anti‑inflammatory property of SVF (expression and suppression of various cytokines and conversion of the macrophage phenotype).

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Anti‑inflammatory effect of stromal vascular fraction cells in fat transplantation

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Abstract

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