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Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors

  • Authors:
    • Yang Li
    • Lei Cao
    • Jun Li
    • Zhongyi Sun
    • Chao Liu
    • He Liang
    • Deguo Wang
    • Jiwei Tian
  • View Affiliations / Copyright

    Affiliations: Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China, Central Hospital of Shanghai Songjiang District, Shanghai 201600, P.R. China
  • Pages: 1367-1373
    |
    Published online on: December 12, 2018
       https://doi.org/10.3892/etm.2018.7085
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Abstract

Αssociation of expression levels of tumor suppressor proteins p53 and p16 and inflammatory factors in simulated weightlessness with the degree of lumbar disc degeneration of rats was investigated. Magnetic resonance imaging (MRI) examination was performed for rats in control group and experimental group, and the intervertebral disc of rats in both groups was detected and analyzed using hematoxylin and eosin (H&E) histopathological staining. The mRNA expression levels of proinflammatory factors, interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) were detected, and p53 and p16 mRNA and protein expression levels were detected. MRI results showed significant intervertebral disc injury in experimental group. Results of H&E staining revealed that the intervertebral disc injury in experimental group was more serious with obvious signs of degeneration than that in control group. The mRNA expression levels of inflammatory factors (IL-1β, IL-6 and TNF-α) in rats in experimental group were significantly increased compared with those in control group, indicating that the degree of lumbar disc degeneration of rats in simulated weightlessness is closely related to the inflammatory factors. RT-PCR and western blotting proved that both p53 and p16 mRNA and protein expression levels in experimental group were obviously increased. Results of t-test manifested that there were statistically significant differences in p53 and p16 expression levels between control group and experimental group (P<0.01). The abnormal expression levels of p53 and p16 genes have close association with the degree of lumbar disc degeneration of rats in simulated weightlessness, and the lumbar disc degeneration is also closely related to the increased expression levels of inflammatory factors.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Cao L, Li J, Sun Z, Liu C, Liang H, Wang D and Tian J: Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors. Exp Ther Med 17: 1367-1373, 2019.
APA
Li, Y., Cao, L., Li, J., Sun, Z., Liu, C., Liang, H. ... Tian, J. (2019). Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors. Experimental and Therapeutic Medicine, 17, 1367-1373. https://doi.org/10.3892/etm.2018.7085
MLA
Li, Y., Cao, L., Li, J., Sun, Z., Liu, C., Liang, H., Wang, D., Tian, J."Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors". Experimental and Therapeutic Medicine 17.2 (2019): 1367-1373.
Chicago
Li, Y., Cao, L., Li, J., Sun, Z., Liu, C., Liang, H., Wang, D., Tian, J."Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1367-1373. https://doi.org/10.3892/etm.2018.7085
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Cao L, Li J, Sun Z, Liu C, Liang H, Wang D and Tian J: Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors. Exp Ther Med 17: 1367-1373, 2019.
APA
Li, Y., Cao, L., Li, J., Sun, Z., Liu, C., Liang, H. ... Tian, J. (2019). Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors. Experimental and Therapeutic Medicine, 17, 1367-1373. https://doi.org/10.3892/etm.2018.7085
MLA
Li, Y., Cao, L., Li, J., Sun, Z., Liu, C., Liang, H., Wang, D., Tian, J."Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors". Experimental and Therapeutic Medicine 17.2 (2019): 1367-1373.
Chicago
Li, Y., Cao, L., Li, J., Sun, Z., Liu, C., Liang, H., Wang, D., Tian, J."Influence of microgravity-induced intervertebral disc degeneration of rats on expression levels of p53/p16 and proinflammatory factors". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1367-1373. https://doi.org/10.3892/etm.2018.7085
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