Open Access

Effect of testosterone on the expression of PPARγ mRNA in PCOS patients

  • Authors:
    • Jinghe Cao
    • Gulijianati Maowulieti
    • Tao Yu
  • View Affiliations

  • Published online on: December 14, 2018     https://doi.org/10.3892/etm.2018.7101
  • Pages: 1761-1765
  • Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expression of peroxisome proliferator-activated receptor γ (PPARγ) mRNA in ovarian granulosa cells of patients with polycystic ovary syndrome (PCOS) were explored. Ovarian granulosa cells were extracted from 5 patients with PCOS and 30 normal controls. Expression of PPARγ mRNA in granulosa cells of the 5 PCOS patients (observation group) and 5 normal controls (control group) was detected by RT-qPCR. The remaining 25 cases of normal human ovarian granulosa cells were cultured in vitro for 48 h, followed by cell culture for another 24 h with different concentrations of testosterone, insulin (INS), and rosiglitazone (RGZ). After that, expression of PPARγ mRNA was detected by RT-qPCR. Relative expression level of PPARγ mRNA in the observation group was significantly lower than that in the control group. Compared with testosterone concentration at 10-2 mol/ml, testosterone concentration at 10-3 mol/ml significantly reduced the expression level of PPARγ mRNA. When the INS concentration was 10-5 mol/ml, relative expression level of PPARγ mRNA was significantly higher than that of the control group (P<0.01). Relative expression level of PPARγ mRNA was significantly higher than that of the control group when INS concentration was 10-4 mol/ml. When the concentration of RGZ was 10-4 mol/ml, the relative expression level of PPARγ mRNA was significantly higher than that of the control group (P<0.01). When concentration of RGZ was 10-3 mol/ml, expression level of PPARγ mRNA was significantly increased comparing to that under a RGZ concentration of 10-4 mol/ml or that of the control group (P<0.01). Appropriate concentrations of testosterone can inhibit the expression of PPARγ mRNA in ovarian granulosa cells, and certain concentrations of INS and RGZ can induce the expression of PPARγ mRNA in ovarian granulosa cells. Abnormal expression of PPARγ mRNA in ovarian granulosa cells may be related to the mechanism of PCOS.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Cao J, Maowulieti G and Yu T: Effect of testosterone on the expression of PPARγ mRNA in PCOS patients. Exp Ther Med 17: 1761-1765, 2019.
APA
Cao, J., Maowulieti, G., & Yu, T. (2019). Effect of testosterone on the expression of PPARγ mRNA in PCOS patients. Experimental and Therapeutic Medicine, 17, 1761-1765. https://doi.org/10.3892/etm.2018.7101
MLA
Cao, J., Maowulieti, G., Yu, T."Effect of testosterone on the expression of PPARγ mRNA in PCOS patients". Experimental and Therapeutic Medicine 17.3 (2019): 1761-1765.
Chicago
Cao, J., Maowulieti, G., Yu, T."Effect of testosterone on the expression of PPARγ mRNA in PCOS patients". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1761-1765. https://doi.org/10.3892/etm.2018.7101