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Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension

  • Authors:
    • Shuying Zheng
    • Tianlun Gu
    • Xingjie Bao
    • Jihan Sun
    • Jinshun Zhao
    • Tao Zhang
    • Lina Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Preventive Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Medical School of Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Clinical Medicine, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang 311121, P.R. China, Department of Chronic Disease Control and Prevention, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315000, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1728-1736
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    Published online on: December 17, 2018
       https://doi.org/10.3892/etm.2018.7107
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Abstract

Circular RNAs (circRNAs) have a great potential as clinical biomarkers; however, specific circRNAs with a diagnostic value for essential hypertension (EH) largely remain to be identified. In the present study, the potential application of Homo sapiens (hsa)_circ_0014243, which was identified to be significantly upregulated in whole blood samples of EH patients in a previous microarray profiling study by our group, in the diagnosis of EH was evaluated. Reverse transcription‑quantitative polymerase chain reaction analysis was performed to determine the expression levels of hsa_circ_0014243 and hsa‑microRNA (miR)‑10a‑5p in a total of 178 blood samples collected from 89 healthy controls and 89 patients diagnosed with EH. Divergent primers were designed for circRNAs, while conventional primers were used for miRs. Independent‑samples t‑tests and bivariate correlation analyses were performed to analyze the association between clinical factors influencing EH and hsa_circ_0014243 expression levels. A receiver operating characteristics (ROC) curve was generated to estimate the diagnostic value of hsa_circ_0014243 for EH. Finally, the expression levels of circRNAs and miRNAs were combined to propose a possible prediction model for EH. The results indicated that hsa_circ_0014243 was upregulated in whole blood samples of EH patients compared with that in the controls (P<0.001). Furthermore, the relative expression levels of hsa_circ_0014243 (Δ quantification cycle) were identified to be significantly correlated with age (r=‑0.259, P<0.001), high‑density lipoprotein levels (r=0.196, P=0.009) and glucose levels (r=‑0.204, P=0.006). The area under the ROC curve (AUC) of the model using hsa_circ_0014243 as a predictor was 0.732. Of note, the AUC increased to 0.781 when hsa_circ_0014243 levels were combined with hsa‑miR‑10a‑5p levels as predictors. The present results suggest that hsa_circ_0014243 has a crucial role in the genesis and development of EH, and presents a certain diagnostic capability for EH.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng S, Gu T, Bao X, Sun J, Zhao J, Zhang T and Zhang L: Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension. Exp Ther Med 17: 1728-1736, 2019.
APA
Zheng, S., Gu, T., Bao, X., Sun, J., Zhao, J., Zhang, T., & Zhang, L. (2019). Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension. Experimental and Therapeutic Medicine, 17, 1728-1736. https://doi.org/10.3892/etm.2018.7107
MLA
Zheng, S., Gu, T., Bao, X., Sun, J., Zhao, J., Zhang, T., Zhang, L."Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension". Experimental and Therapeutic Medicine 17.3 (2019): 1728-1736.
Chicago
Zheng, S., Gu, T., Bao, X., Sun, J., Zhao, J., Zhang, T., Zhang, L."Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1728-1736. https://doi.org/10.3892/etm.2018.7107
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng S, Gu T, Bao X, Sun J, Zhao J, Zhang T and Zhang L: Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension. Exp Ther Med 17: 1728-1736, 2019.
APA
Zheng, S., Gu, T., Bao, X., Sun, J., Zhao, J., Zhang, T., & Zhang, L. (2019). Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension. Experimental and Therapeutic Medicine, 17, 1728-1736. https://doi.org/10.3892/etm.2018.7107
MLA
Zheng, S., Gu, T., Bao, X., Sun, J., Zhao, J., Zhang, T., Zhang, L."Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension". Experimental and Therapeutic Medicine 17.3 (2019): 1728-1736.
Chicago
Zheng, S., Gu, T., Bao, X., Sun, J., Zhao, J., Zhang, T., Zhang, L."Circular RNA hsa_circ_0014243 may serve as a diagnostic biomarker for essential hypertension". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1728-1736. https://doi.org/10.3892/etm.2018.7107
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