Biodegradable ciprofloxacin‑incorporated waterborne polyurethane polymers prevent bacterial biofilm formation in vitro

Xu,Yuchen; Wang,Jianzhong; Hao,Zongyao; Wang,Sai; Liang,Chaozhao

doi:10.3892/etm.2018.7113

Biodegradable ciprofloxacin‑incorporated waterborne polyurethane polymers prevent bacterial biofilm formation in vitro

Authors:
- Yuchen Xu
- Jianzhong Wang
- Zongyao Hao
- Sai Wang
- Chaozhao Liang
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Affiliations: Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
Published online on: December 19, 2018 https://doi.org/10.3892/etm.2018.7113
Pages: 1831-1836

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Abstract

The aim of the present study was to explore whether ciprofloxacin‑incorporated waterborne polyurethane (WBPU) polymers have the capacity to inhibit bacterial biofilm formation in vitro. WBPU polymers were incorporated with ciprofloxacin and were cultured with Escherichia coli (E. coli) or Staphylococcus aureus (S. aureus) in media for 2, 4 or 7 days. In another experiment, the WBPU membranes were cultured with Proteus mirabilis (P. mirabilis) in artificial urine for 2, 4 or 7 days. Colony counting, scanning electron microscopy and fluorescence confocal microscopy were utilized to examine bacterial biofilms on the surfaces of membranes. The membranes were further co‑cultured with P. mirabilis in a simple model of an artificial catheterized bladder in order to evaluate their ability to control encrustation. The WBPU films with ciprofloxacin effectively inhibited bacterial biofilm formation in the culture medium and in artificial urine. In addition, in artificial urine, the films with ciprofloxacin reduced catheter obstruction. In conclusion, ciprofloxacin‑incorporated WBPU polymers are able to effectively inhibit bacterial biofilm formation in vitro.

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1	Orthopedic implants-a global market overview. Indus Exp. 2011.
2	Koseoglu H, Aslan G, Esen N, Sen BH and Coban H: Ultrastructural stages of biofilm development of Escherichia coli on urethral catheters and effects of antibiotics on biofilm formation. Urology. 68:942–946. 2006. View Article : Google Scholar : PubMed/NCBI
3	Veerachamy S, Yarlagadda T, Manivasagam G and Yarlagadda PK: Bacterial adherence and biofilm formation on medical implants: A review. Proc Inst Mech Eng H. 228:1083–1099. 2014. View Article : Google Scholar : PubMed/NCBI
4	Cheng G, Zhang Z, Chen S, Bryers JD and Jiang S: Inhibition of bacterial adhesion and biofilm formation on zwitterionic surfaces. Biomaterials. 28:4192–4199. 2007. View Article : Google Scholar : PubMed/NCBI
5	Magana M, Sereti C, Ioannidis A, Mitchell CA, Ball AR, Magiorkinis E, Chatzipanagiotou S, Hamblin MR, Hadjifrangiskou M and Tegos GP: Options and limitations in clinical investigation of bacterial biofilms. Clin Microbiol Rev. 31(pii): e00084–16. 2018.PubMed/NCBI
6	Tai Z, Ma H, Liu B, Yan X and Xue Q: Facile synthesis of Ag/GNS-g-PAA nanohybrids for antimicrobial applications. Colloids Surf B Biointerfaces. 89:147–151. 2012. View Article : Google Scholar : PubMed/NCBI
7	Sivakumar PM, Iyer G, Natesan L and Doble M: 3′-Hydroxy-4-methoxychalcone as a potential antibacterial coating on polymeric biomaterials. Appl Surf Sci. 256:6018–6024. 2010. View Article : Google Scholar
8	Kowalczuk D, Ginalska G and Golus J: Characterization of the developed antimicrobial urological catheters. Int J Pharm. 402:175–183. 2010. View Article : Google Scholar : PubMed/NCBI
9	Kara F, Aksoy EA, Yuksekdag Z, Hasirci N and Aksoy S: Synthesis and surface modification of polyurethanes with chitosan for antibacterial properties. Carbohydr Polym. 112:39–47. 2014. View Article : Google Scholar : PubMed/NCBI
10	Wang Z, Yu L, Ding M, Tan H, Li J and Fu Q: Preparation and rapid degradation of nontoxic biodegradable polyurethanes based on poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) and l -lysine diisocyanate. Polym Chem. 2-3:6012011. View Article : Google Scholar
11	Jiang X, Yu F, Wang Z, Li J, Tan H, Ding M and Fu Q: Fabrication and characterization of waterborne biodegradable polyurethanes 3-dimensional porous scaffolds for vascular tissue engineering. J Biomater Sci Polym Ed. 21:1637–1652. 2010. View Article : Google Scholar : PubMed/NCBI
12	Kang SY, Ji Z, Tseng LF, Turner SA, Villanueva DA, Johnson R, Albano A and Langer R: Design and synthesis of waterborne polyurethanes. Adv Mater. 30:e17062372018. View Article : Google Scholar : PubMed/NCBI
13	Sivak WN: Synthesis and characterization of novel polyurethane drug delivery systems. Dissertations & Theses-Gradworks. 2007.
14	Sivak WN, Zhang J, Petoud S and Beckman EJ: Simultaneous drug release at different rates from biodegradable polyurethane foams. Acta Biomater. 5:2398–2408. 2009. View Article : Google Scholar : PubMed/NCBI
15	Jiang X, Li J, Ding M, Tan H, Ling Q, Zhong Y and Fu Q: Synthesis and degradation of nontoxic biodegradable waterborne polyurethanes elastomer with poly(ε-caprolactone) and poly(ethylene glycol) as soft segment. Eur Polym J. 43:1838–1846. 2007. View Article : Google Scholar
16	Wang J, Liu Q, Tian Y, Jian Z, Li H and Wang K: Biodegradable hydrophilic polyurethane PEGU25 loading antimicrobial peptide Bmap-28: A sustained-release membrane able to inhibit bacterial biofilm formation in vitro. Sci Rep. 5:86342015. View Article : Google Scholar : PubMed/NCBI
17	Ye T, Jian Z, Wang J, He W, Liu Q, Wang K, Li H and Tan H: Antimicrobial activity study of triclosan-loaded WBPU on Proteus mirabilis in vitro. Int Urol Nephrol. 49:563–571. 2017. View Article : Google Scholar : PubMed/NCBI
18	Griffith DP, Musher DM and Itin C: Urease. The primary cause of infection-induced urinary stones. Invest Urol. 13:346–350. 1976.PubMed/NCBI
19	Morris NS, Stickler DJ and Winters C: Which indwelling urethral catheters resist encrustation by Proteus mirabilis biofilms? Br J Urol. 80:58–63. 1997. View Article : Google Scholar : PubMed/NCBI
20	Römling U and Balsalobre C: Biofilm infections, their resilience to therapy and innovative treatment strategies. J Intern Med. 272:541–561. 2012. View Article : Google Scholar : PubMed/NCBI
21	Costerton JW, Stewart PS and Greenberg EP: Bacterial biofilms: A common cause of persistent infections. Science. 284:1318–1322. 1999. View Article : Google Scholar : PubMed/NCBI
22	Lebeaux D, Ghigo JM and Beloin C: Biofilm-related infections: Bridging the gap between clinical management and fundamental aspects of recalcitrance toward antibiotics. Microbiol Mol Biol Rev. 78:510–543. 2014. View Article : Google Scholar : PubMed/NCBI
23	Darouiche RO, Raad II, Heard SO, Thornby JI, Wenker OC, Gabrielli A, Berg J, Khardori N, Hanna H, Hachem R, et al: A comparison of two antimicrobial-impregnated central venous catheters. Catheter Study Group. N Engl J Med. 340:1–8. 1999. View Article : Google Scholar : PubMed/NCBI
24	Ruggeri V, Francolini I, Donelli G and Piozzi A: Synthesis, characterization, and in vitro activity of antibiotic releasing polyurethanes to prevent bacterial resistance. J Biomed Mater Res A. 81:287–298. 2007. View Article : Google Scholar : PubMed/NCBI
25	Zhou L, Yu L, Ding M, Li J, Tan H, Wang Z and Fu Q: Synthesis and characterization of pH-sensitive biodegradable polyurethane for potential drug delivery applications. Macromolecules. 44:857–864. 2011. View Article : Google Scholar
26	Cakić SM, Ristić IS, Krakovský I, Stojiljković DT, Bělský P and Kollová L: Crystallization and thermal properties in waterborne polyurethane elastomers: Influence of mixed soft segment block. Mater Chem Phys. 144:31–40. 2014. View Article : Google Scholar
27	Williams GJ and Stickler DJ: Some observations on the diffusion of antimicrobial agents through the retention balloons of foley catheters. J Urol. 178:697–701. 2007. View Article : Google Scholar : PubMed/NCBI
28	Stickler DJ, Lear JC, Morris NS, Macleod SM, Downer A, Cadd DH and Feast WJ: Observations on the adherence of Proteus mirabilis onto polymer surfaces. J Appl Microbiol. 100:1028–1033. 2006. View Article : Google Scholar : PubMed/NCBI
29	Morris NS, Stickler DJ and McLean RJ: The development of bacterial biofilms on indwelling urethral catheters. World J Urol. 17:345–350. 1999. View Article : Google Scholar : PubMed/NCBI
30	Stickler D, Ganderton L, King J, Nettleton J and Winters C: Proteus mirabilis biofilms and the encrustation of urethral catheters. Urol Res. 21:407–411. 1993. View Article : Google Scholar : PubMed/NCBI
31	Suller MT, Anthony VJ, Mathur S, Feneley RC, Greenman J and Stickler DJ: Factors modulating the pH at which calcium and magnesium phosphates precipitate from human urine. Urol Res. 33:254–260. 2005. View Article : Google Scholar : PubMed/NCBI