Plasma microRNA: A novel non‑invasive biomarker for HBV‑associated liver fibrosis staging

  • Authors:
    • Tie‑Zheng Wang
    • Dong‑Dong Lin
    • Bo‑Xun Jin
    • Xiang‑Ying Sun
    • Ning Li
  • View Affiliations

  • Published online on: December 19, 2018     https://doi.org/10.3892/etm.2018.7117
  • Pages: 1919-1929
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Abstract

The aim of the present study was to evaluate the potential use of 7 plasma miRNAs for liver fibrosis staging in patients with chronic hepatitis B virus (HBV) infection. Relative levels of miRNAs were measured using quantitative polymerase chain reaction and used to develop a diagnostic panel. A receiver operating characteristic (ROC) curve was drawn to evaluate the performance of individual miRNAs and the whole panel. It was identified that hsa‑miR‑122 exhibited significantly different expression levels between F4 and F3, F2, F1, and F0 fibrosis stages (P<0.05), and between F2 and F1 stages (P=0.045); hsa‑miR‑146a‑5p, hsa‑miR‑29c‑3p and hsa‑miR‑223 exhibited significantly different expression levels between F4 and F0 stages. ROC analysis revealed that hsa‑miR‑122‑5p, hsa‑miR‑223 and hsa‑miR‑29c‑3p identified patients with ≥F2 fibrosis with area under the curve (AUC) =0.745, 0.631 and 0.670, respectively. hsa‑miR‑122‑5p identified patients with ≥F3 disease (AUC=0.783). hsa‑miR‑122‑5p, hsa‑miR‑223 and hsa‑miR‑29c‑3p identified patients with cirrhosis with AUC=0.776, 0.617 and 0.619, respectively. The miRNA panel exhibited a higher accuracy compared with individual miRNAs in discriminating between ≥F2, ≥F3 and F4 fibrosis stages with AUC=0.904, 0.889 and 0.835, respectively. hsa‑miR‑122‑5p, hsa‑miR‑146a, hsa‑miR‑29c and hsa‑miR‑223 were positively correlated with fibrosis stage. hsa‑miR‑122‑5p and hsa‑miR‑381‑3p were negatively correlated with alanine aminotransferase, aspartate transaminase and HBV viral DNA load. These 7 miRNAs may serve as potential biomarkers of liver fibrosis in patients with HBV‑associated fibrosis. The miRNA panel may serve as a novel non‑invasive method for liver fibrosis staging.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang TZ, Lin DD, Jin BX, Sun XY and Li N: Plasma microRNA: A novel non‑invasive biomarker for HBV‑associated liver fibrosis staging. Exp Ther Med 17: 1919-1929, 2019
APA
Wang, T., Lin, D., Jin, B., Sun, X., & Li, N. (2019). Plasma microRNA: A novel non‑invasive biomarker for HBV‑associated liver fibrosis staging. Experimental and Therapeutic Medicine, 17, 1919-1929. https://doi.org/10.3892/etm.2018.7117
MLA
Wang, T., Lin, D., Jin, B., Sun, X., Li, N."Plasma microRNA: A novel non‑invasive biomarker for HBV‑associated liver fibrosis staging". Experimental and Therapeutic Medicine 17.3 (2019): 1919-1929.
Chicago
Wang, T., Lin, D., Jin, B., Sun, X., Li, N."Plasma microRNA: A novel non‑invasive biomarker for HBV‑associated liver fibrosis staging". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1919-1929. https://doi.org/10.3892/etm.2018.7117