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Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms

  • Authors:
    • Juanzi Zhang
    • Wenxian Hu
    • Qixian Diao
    • Zhaowei Wang
    • Jin Miao
    • Xiao Chen
    • Zhijun Xue
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, The Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, Shandong 266033, P.R. China, Department of General Surgery, The Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, Shandong 266033, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Pages: 1643-1648
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    Published online on: December 24, 2018
       https://doi.org/10.3892/etm.2018.7133
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Abstract

The present study aimed to explore the therapeutic effect and underlying mechanism of epidermal growth factor (EGF) on the wound healing of diabetic foot ulcers (DFU). A total of 48 rabbits with DFU were randomly divided into 2 groups, comprising the treatment and control groups. Full‑thickness skin (10x10 mm) was excised from the thigh of each rabbit. The wounds in the treatment group were treated with 100 mg/l EGF once a day for 1 month. The control group received no treatment. At 20 days following treatment, new granulation tissues that formed beyond the edge of the wound were collected for subsequent analysis. Tissues from rabbits in the treatment group produced a greater number of fibroblasts, which exhibited a fibroblastic morphology when compared with those in the control group. In the treatment group, a larger number of these fibroblasts were observed as clusters, and there were numerous blood vessels when compared with the control group. The fibroblasts in the control group exhibited an irregular morphology, contained fewer organelles and the surrounding collagenous fibers were sparse. These fibroblasts also demonstrated a disordered arrangement and it was revealed that the wound healed at a slower rate compared with the treatment group. Endogenous EGF mRNA detection revealed that there was a significant difference (P<0.05) in the relative gray value of EGF mRNA between the treatment (103.27±4.27) and control (63.88±4.36) groups. In conclusion, EGF may accelerate the healing of DFU, and exogenous EGF treatment may upregulate the expression of EGF mRNA in newly generated tissues.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Hu W, Diao Q, Wang Z, Miao J, Chen X and Xue Z: Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms. Exp Ther Med 17: 1643-1648, 2019.
APA
Zhang, J., Hu, W., Diao, Q., Wang, Z., Miao, J., Chen, X., & Xue, Z. (2019). Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms. Experimental and Therapeutic Medicine, 17, 1643-1648. https://doi.org/10.3892/etm.2018.7133
MLA
Zhang, J., Hu, W., Diao, Q., Wang, Z., Miao, J., Chen, X., Xue, Z."Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms". Experimental and Therapeutic Medicine 17.3 (2019): 1643-1648.
Chicago
Zhang, J., Hu, W., Diao, Q., Wang, Z., Miao, J., Chen, X., Xue, Z."Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1643-1648. https://doi.org/10.3892/etm.2018.7133
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Hu W, Diao Q, Wang Z, Miao J, Chen X and Xue Z: Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms. Exp Ther Med 17: 1643-1648, 2019.
APA
Zhang, J., Hu, W., Diao, Q., Wang, Z., Miao, J., Chen, X., & Xue, Z. (2019). Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms. Experimental and Therapeutic Medicine, 17, 1643-1648. https://doi.org/10.3892/etm.2018.7133
MLA
Zhang, J., Hu, W., Diao, Q., Wang, Z., Miao, J., Chen, X., Xue, Z."Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms". Experimental and Therapeutic Medicine 17.3 (2019): 1643-1648.
Chicago
Zhang, J., Hu, W., Diao, Q., Wang, Z., Miao, J., Chen, X., Xue, Z."Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1643-1648. https://doi.org/10.3892/etm.2018.7133
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