Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
March-2019 Volume 17 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2019 Volume 17 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing

  • Authors:
    • Liusheng Lai
    • Xianqing Zhou
    • Huaizhou Chen
    • Yadan Luo
    • Weiguo Sui
    • Jiaxing Zhang
    • Donge Tang
    • Qiang Yan
    • Yong Dai
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Guilin No. 924 Hospital, Guangxi Key Laboratory of Metabolic Disease Research, Guilin Key Laboratory of Kidney Disease Research, Guilin, Guangxi 541002, P.R. China, Clinical Medical Research Center of The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China
    Copyright: © Lai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2206-2220
    |
    Published online on: January 17, 2019
       https://doi.org/10.3892/etm.2019.7183
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to assess the genetic diversity of the B‑cell receptor (BCR) in kidney transplant recipients with acute rejection. A total of three patients with acute rejection after kidney transplantation were examined by performing a composition and diversity analysis of the BCR immunoglobulin heavy chain (IGH) complementarity‑determining region 3 (H‑CDR3) repertoire. The peripheral blood mononuclear cells of patients were collected at 1 day prior to (Pre1), as well as 1 day (Post1) and 7 days (Post7) after the transplantation, and DNA was extracted. High‑throughput sequencing technology was applied to determine the BCR repertoire. Raw sequences in FASTQ format were analyzed with the Basic Local Alignment Search Tool. The diversity of the BCR repertoire was assessed by calculating Shannon entropy, Simpson's diversity index, the Gini coefficient and highly expanded clone distributions. The diversity of the BCR repertoire at Pre1 was greater than that at Post1 or Post7. The diversity of the BCR repertoire was the lowest at Post1 and increased at Post7 but failed to reach the pre‑transplantation levels. Patients exhibited the loss of seven IGH variable (IGHV)3 family genes, while five new genes were expressed at a low frequency. Furthermore, five IGHV‑IGH joining (IGHJ) gene pairings, including IGHJ6‑IGHV3‑11, were detected in the patients. Up‑ and downregulated genes were assessed by calculating the expression frequencies of the IGH diversity and IGHV gene families at Post1 and Post7. The results of the H‑CDR3 length distribution and H‑CDR3 amino acid (AA) usage analyses indicated that in Case 1 and 2, the AA length was similar at mostly 14‑18 AA, while that in Case 3 was relatively stable at 12‑16 AA. In conclusion, the present results illustrate the diversity of H‑CDR3 in patients with acute rejection after kidney transplantation may provide novel ideas, methods and means of monitoring and analyzing the immune status of patients under physiological and pathological conditions.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Figure 12

View References

1 

Liyanage T, Ninomiya T, Perkovic V, Woodward M, Stirnadel-Farrant H, Matsushita K, Iseki K, Seong HL, Monaghan H and Jha V: Chronic kidney disease in Asia: Protocol for a collaborative overview. Nephrology (Carlton). 22:456–462. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Roodnat JI, Hilbrands LB, Hené RJ, de Sévaux RG, Smak Gregoor PJ, Kal-van Gestel JA, Konijn C, van Zuilen A, van Gelder T, Hoitsma AJ and Weimar W: 15-year follow-up of a multicenter, randomized, calcineurin inhibitor withdrawal study in kidney transplantation. Transplantation. 98:47–53. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Ota K: Organ transplantation-approching the 21st century. Nihon Geka Gakkai Zasshi. 99:110–112. 1998.(In Japanese). PubMed/NCBI

4 

Yilmaz S: Chronic allograft nephropathy (chronic allograft damage): Can it be avoided? Curr Transpl Rep. 1:91–99. 2014. View Article : Google Scholar

5 

Shahbazi F, Ranjbaran M, Karamifar S, Soori H and Manesh HJ: Graft survival rate of renal transplantation during a period of 10 years in Iran. J Res Med Sci. 20:1046–1052. 2015. View Article : Google Scholar : PubMed/NCBI

6 

San Segundo D, Rodrigo E, Kislikova M, Ruiz JC, Fernandez-Fresnedo G, Asensio E, Arias M and Lopez-Hoyos M: Frequencies of circulating B-cell subpopulations before kidney transplantation identify patients at risk of acute rejection. Transplant Proc. 47:54–56. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Ming M, Zhao B, Qiang L and He YY: Effect of immunosuppressants tacrolimus and mycophenolate mofetil on the keratinocyte UVB response. Photochem Photobiol. 91:242–247. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Velásquez SY, Arias LF, García LF and Alvarez CM: T cell receptor beta chain (TCR-Vbeta) repertoire of circulating CD4(+) CD25(−), CD4(+) CD25(low) and CD4(+) CD25(high) T cells in patients with long-term renal allograft survival. Transpl Int. 23:54–63. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Matsutani T, Ohashi Y, Yoshioka T, Tsuruta Y, Doi H, Satomi S and Suzuki R: Skew in T-cell receptor usage and clonal T-cell expansion in patients with chronic rejection of transplanted kidneys. Transplantation. 75:398–407. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Lai L, Wang L, Chen H, Zhang J, Yan Q, Ou M, Lin H, Hou X, Chen S, Dai Y and Sui W: T cell repertoire following kidney transplantation revealed by high-throughput sequencing. Transpl Immunol. 39:34–45. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Zhang W, Du Y, Su Z, Wang C, Zeng X, Zhang R, Hong X, Nie C, Wu J, Cao H, et al: IMonitor: A robust pipeline for TCR and BCR repertoire analysis. Genetics. 201:459–472. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Zan H and Casali P: Editorial: Epigenetics of B cells and antibody responses. Front ImmunoL. 6:6562015. View Article : Google Scholar : PubMed/NCBI

13 

Bican Demir A, Erer Özbek S, Bora I, Hakyemez B, Tırnova I and Kaya E: Two cases with developing neurologic complications after liver transplant. Exp Clin Transplant. 14:685–687. 2016.PubMed/NCBI

14 

Racusen LC, Solez K, Colvin RB, Bonsib SM, Castro MC, Cavallo T, Croker BP, Demetris AJ, Drachenberg CB, Fogo AB, et al: The Banff 97 working classification of renal allograft pathology. Kidney Int. 55:713–723. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Galson JD, Pollard AJ, Trück J and Kelly DF: Studying the antibody repertoire after vaccination: Practical applications. Trends Immunol. 35:319–331. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Pickman Y, Dunn-walters D and Mehr R: BCR CDR3 length distributions differ between blood and spleen and between old and young patients, and TCR distributions can be used to detect myelodysplastic syndrome. Phys Biol. 10:0560012013. View Article : Google Scholar : PubMed/NCBI

17 

Wang Z, Yang H, Liu X, Zhang J, Han Z, Tao J, Zhao C, Ju X, Tan R and Gu M: Role of B and T lymphocyte attenuator in renal transplant recipients with biopsy-proven acute rejection. Med Sci Monit. 24:387–396. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Gibson KL, Wu YC, Barnett Y, Duggan O, Vaughan R, Kondeatis E, Nilsson BO, Wikby A, Kipling D and Dunn-Walters DK: B-cell diversity decreases in old age and is correlated with poor health status. Aging Cell. 8:18–25. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Lee YN, Frugoni F, Dobbs K, Tirosh I, Du L, Ververs FA, Ru H, Ott de Bruin L, Adeli M, Bleesing JH, et al: Characterization of T and B cell repertoire diversity in patients with RAG deficiency. Sci Immunol. 1:eaah61092016. View Article : Google Scholar : PubMed/NCBI

20 

Berthelot JM, Jamin C, Amrouche K, Le Goff B, Maugars Y and Youinou P: Regulatory B cells play a key role in immune system balance. Joint Bone Spine. 80:18–22. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Newell KA, Asare A, Kirk AD, Gisler TD, Bourcier K, Suthanthiran M, Burlingham WJ, Marks WH, Sanz I, Lechler RI, et al: Identification of a B cell signature associated with renal transplant tolerance in humans. J Clin Invest. 120:1836–1847. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Ma L, Wang X, Bi X, Yang J, Shi B, He X, Ma R, Ma Q and Yao X: Characteristics peripheral blood IgG and IgM heavy chain complementarity determining region 3 repertoire before and after immunization with recombinant HBV vaccine. PLoS One. 12:e1704792017.

23 

Roy B, Neumann RS, Snir O, Iversen R, Sandve GK, Lundin KEA and Sollid LM: High-throughput single-cell analysis of B cell receptor usage among autoantigen-specific plasma cells in celiac disease. J Immunol. 199:782–791. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Jeon HJ, Fang T, Lee JG, Jang JY, Kim K, Choi S, Yan JJ, Ryu JH, Koo TY, Ahn C and Yang J: VDJ gene usage of B cell receptors in peripheral blood of ABO-incompatible kidney transplantation patients. Transplant Proc. 50:1056–1062. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Shi B, Yu J, Ma L, Ma Q, Liu C, Sun S, Ma R and Yao X: Short-term assessment of BCR repertoires of SLE patients after high dose glucocorticoid therapy with high-throughput sequencing. Springerplus. 5:752016. View Article : Google Scholar : PubMed/NCBI

26 

Dohmen SE, Muit J, Ligthart PC, Verhagen OJ and van der Schoot CE: Anti-e found in a case of hemolytic disease of the fetus and newborn make use of the IGHV3 superspecies genes. Transfusion. 48:194–195. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Beausang JF, Fan HC, Sit R, Hutchins MU, Jirage K, Curtis R, Hutchins E, Quake SR and Yabu JM: B cell repertoires in HLA-sensitized kidney transplant candidates undergoing desensitization therapy. J Transl Med. 15:92017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lai L, Zhou X, Chen H, Luo Y, Sui W, Zhang J, Tang D, Yan Q and Dai Y: Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing. Exp Ther Med 17: 2206-2220, 2019.
APA
Lai, L., Zhou, X., Chen, H., Luo, Y., Sui, W., Zhang, J. ... Dai, Y. (2019). Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing. Experimental and Therapeutic Medicine, 17, 2206-2220. https://doi.org/10.3892/etm.2019.7183
MLA
Lai, L., Zhou, X., Chen, H., Luo, Y., Sui, W., Zhang, J., Tang, D., Yan, Q., Dai, Y."Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing". Experimental and Therapeutic Medicine 17.3 (2019): 2206-2220.
Chicago
Lai, L., Zhou, X., Chen, H., Luo, Y., Sui, W., Zhang, J., Tang, D., Yan, Q., Dai, Y."Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2206-2220. https://doi.org/10.3892/etm.2019.7183
Copy and paste a formatted citation
x
Spandidos Publications style
Lai L, Zhou X, Chen H, Luo Y, Sui W, Zhang J, Tang D, Yan Q and Dai Y: Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing. Exp Ther Med 17: 2206-2220, 2019.
APA
Lai, L., Zhou, X., Chen, H., Luo, Y., Sui, W., Zhang, J. ... Dai, Y. (2019). Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing. Experimental and Therapeutic Medicine, 17, 2206-2220. https://doi.org/10.3892/etm.2019.7183
MLA
Lai, L., Zhou, X., Chen, H., Luo, Y., Sui, W., Zhang, J., Tang, D., Yan, Q., Dai, Y."Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing". Experimental and Therapeutic Medicine 17.3 (2019): 2206-2220.
Chicago
Lai, L., Zhou, X., Chen, H., Luo, Y., Sui, W., Zhang, J., Tang, D., Yan, Q., Dai, Y."Composition and diversity analysis of the B‑cell receptor immunoglobulin heavy chain complementarity‑determining region 3 repertoire in patients with acute rejection after kidney transplantation using high‑throughput sequencing". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2206-2220. https://doi.org/10.3892/etm.2019.7183
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team