Open Access

Comparison of different cell culture plates for the enrichment of non‑adherent human mononuclear cells

  • Authors:
    • Annett Klinder
    • Jana Markhoff
    • Anika Jonitz‑Heincke
    • Philipp Sterna
    • Achim Salamon
    • Rainer Bader
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  • Published online on: January 25, 2019     https://doi.org/10.3892/etm.2019.7204
  • Pages: 2004-2012
  • Copyright: © Klinder et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

While tissue‑resident monocytes and macrophages are considered to be vital players in the in vivo interaction between biomaterials and surrounding tissue, their isolation is limited. In order to establish in vitro models elucidating implant and tissue interactions, peripheral blood mononuclear cells (PBMCs) represent a viable source for bone marrow‑derived monocytes and an alternative to tissue‑resident cells. The aim of present study was to analyse different adhesion‑preventing tissue culture plates for their potential to facilitate the culture of monocytes without differentiation into macrophages. Freshly isolated PBMCs were seeded into four commercially available tissue culture plates with different adhesive properties and were tested for surface CD14 and CD68 expression using flow cytometry following 7 days in culture. When PBMCs were cultivated in RPMI on Cellstar® Cell culture plates with Cell‑Repellent Surface, a significant increase in CD14‑positive cells was observed compared with cultivation in standard tissue culture‑treated plates. This was accompanied by elevated cytokine production of interleukin‑6 (IL6) and interleukin‑8 (IL8); however, overall cell growth was not affected. When PBMCs were pre‑cultured in cell‑repellent plates, there was a higher yield of adherent cells after subsequent transfer into standard tissue culture‑treated plates. Cultivation of PBMCs on cell‑repellent culture plates favoured a monocytic phenotype and thus, represents an alternative to increase the fraction of monocytes yielded from PBMCs.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Klinder A, Markhoff J, Jonitz‑Heincke A, Sterna P, Salamon A and Bader R: Comparison of different cell culture plates for the enrichment of non‑adherent human mononuclear cells . Exp Ther Med 17: 2004-2012, 2019
APA
Klinder, A., Markhoff, J., Jonitz‑Heincke, A., Sterna, P., Salamon, A., & Bader, R. (2019). Comparison of different cell culture plates for the enrichment of non‑adherent human mononuclear cells . Experimental and Therapeutic Medicine, 17, 2004-2012. https://doi.org/10.3892/etm.2019.7204
MLA
Klinder, A., Markhoff, J., Jonitz‑Heincke, A., Sterna, P., Salamon, A., Bader, R."Comparison of different cell culture plates for the enrichment of non‑adherent human mononuclear cells ". Experimental and Therapeutic Medicine 17.3 (2019): 2004-2012.
Chicago
Klinder, A., Markhoff, J., Jonitz‑Heincke, A., Sterna, P., Salamon, A., Bader, R."Comparison of different cell culture plates for the enrichment of non‑adherent human mononuclear cells ". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2004-2012. https://doi.org/10.3892/etm.2019.7204