Assessing biological aging following systemic administration of bFGF‑supplemented adipose‑derived stem cells with high efficacy in an experimental rat model

Bae,Hahn‑Sol; Son,Hye‑Youn; Son,Youngsook; Kim,Sundong; Hong,Hyun‑Sook; Park,Ji‑Ung

doi:10.3892/etm.2019.7251

Assessing biological aging following systemic administration of bFGF‑supplemented adipose‑derived stem cells with high efficacy in an experimental rat model

Authors:
- Hahn‑Sol Bae
- Hye‑Youn Son
- Youngsook Son
- Sundong Kim
- Hyun‑Sook Hong
- Ji‑Ung Park
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Affiliations: Department of Plastic and Reconstructive Surgery, Seoul National University Boramae Medical Center, Seoul 07061, Republic of Korea, Department of Genetic Engineering, Graduate School of Biotechnology, Kyung Hee University, Yongin, Gyeonggi 16979, Republic of Korea, Senior Science Life Corporation, Seoul 08594, Republic of Korea, Kyung Hee Institute for Regenerative Medicine, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
Published online on: February 7, 2019 https://doi.org/10.3892/etm.2019.7251
Pages: 2407-2416
Copyright: © Bae et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Biological aging (BA) is a tool for comprehensive assessment of individual health status. A rat model was developed for measuring BA by intravenously administering adipose‑derived stem cells (ADSCs) into rats and evaluating several biochemical parameters. In addition, the effect of basic fibroblast growth factor (bFGF) on the differentiation potential of ADSCs was analyzed. A total of 12 male Sprague Dawley rats were divided into autologous ADSC administration (n=6) and saline administration (n=6) groups. The ADSC administration group was further divided into the bFGF supplemented (n=3) and bFGF non‑supplemented (n=3) groups. Biochemical parameters and antioxidant potential were evaluated prior to fat harvest and ADSC administration, as well as 1, 3, and 5 weeks following ADSC administration. ADSC administration regulated inflammation, renal and hepatic functions, and levels of antioxidant enzymes. The cell doubling time of the bFGF‑supplemented group was shorter (P=0.0001) than that of the bFGF non‑supplemented group. Renal and hepatic functions were maintained with bFGF supplementation, which possibly enhanced the effect of ADSCs. The rat model developed in the present study may promote better understanding of BA in the context of bFGF‑supplemented ADSC administration.

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