Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
April-2019 Volume 17 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2019 Volume 17 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway

  • Authors:
    • Danyan Chen
    • Xiaolong Huang
    • Song Lu
    • Huacong Deng
    • Hua Gan
    • Rongxi Huang
    • Binghan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology and Nephrology, The Zhongshan District of Chongqing General Hospital, Chongqing 400231, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2465-2472
    |
    Published online on: February 12, 2019
       https://doi.org/10.3892/etm.2019.7256
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study examined the potential function and underlying mechanisms of microRNA‑125a (miR‑125a) in thyroiditis. Mice were subcutaneously administered with 100 µg porcine thyroglobulin weekly for 2 weeks to establish the thyroiditis model. Results of the in vivo study demonstrated that miR‑125a serum expression was upregulated in thyroiditis mice compared with the control group. In vitro studies were performed on a mouse macrophage cell line in which a model of thyroiditis was established using 10 ng/ml human interferon‑γ. Upregulated miR‑125a expression was achieved via mimic transfection. Increased miR‑125a expression reduced autophagy and cell proliferation, increased the apoptotic rate and the expression of pro‑inflammatory factors tumor necrosis factor‑α, interleukin (IL)‑1β, IL‑6 and IL‑18 via downregulation of the phosphoinositide 3‑kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway. PI3K inhibition enhanced the ability of miR‑125a to increase the inflammatory response in vitro via regulation of the PI3K/Akt/mTOR signaling pathway. These results suggest miR‑125a inhibited autophagy in a model of thyroiditis through the PI3K/Akt/mTOR signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Byun JI, Lee ST, Jung KH, Sunwoo JS, Moon J, Lim JA, Lee DY, Shin YW, Kim TJ, Lee KJ, et al: Effect of immunotherapy on seizure outcome in patients with autoimmune encephalitis: A prospective observational registry study. PLoS One. 11:e01464552016. View Article : Google Scholar : PubMed/NCBI

2 

Winther KH, Watt T, Bjørner JB, Cramon P, Feldt-Rasmussen U, Gluud C, Gram J, Groenvold M, Hegedüs L, Knudsen N, et al: The chronic autoimmune thyroiditis quality of life selenium trial (CATALYST): Study protocol for a randomized controlled trial. Trials. 15:1152014. View Article : Google Scholar : PubMed/NCBI

3 

Nazarpour S, Ramezani Tehrani F, Simbar M, Tohidi M, Alavi Majd H and Azizi F: Effects of levothyroxine treatment on pregnancy outcomes in pregnant women with autoimmune thyroid disease. Eur J Endocrinol. 176:253–265. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Kim HM, Kim ES and Koo JS: Expression of autophagy-related proteins in different types of thyroid cancer. Int J Mol Sci. 18:E5402017. View Article : Google Scholar : PubMed/NCBI

5 

Singh BK, Sinha RA, Ohba K and Yen PM: Role of thyroid hormone in hepatic gene regulation, chromatin remodeling, and autophagy. Mol Cell Endocrinol. 458:160–168. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Lin CI, Whang EE, Donner DB, Du J, Lorch J, He F, Jiang X, Price BD, Moore FD Jr and Ruan DT: Autophagy induction with RAD001 enhances chemosensitivity and radiosensitivity through Met inhibition in papillary thyroid cancer. Mol Cancer Res. 8:1217–1226. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Kim SH, Kang JG, Kim CS, Ihm SH, Choi MG, Yoo HJ and Lee SJ: Synergistic cytotoxicity of BIIB021 with triptolide through suppression of PI3K/Akt/mTOR and NF-κB signal pathways in thyroid carcinoma cells. Biomed Pharmacother. 83:22–32. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Pringle DR, Vasko VV, Yu L, Manchanda PK, Lee AA, Zhang X, Kirschner JM, Parlow AF, Saji M, Jarjoura D, et al: Follicular thyroid cancers demonstrate dual activation of PKA and mTOR as modeled by thyroid-specific deletion of Prkarla and Pten in mice. J Clin Endocrinol Metab. 99:E804–E812. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Souza EC, Ferreira AC and Carvalho DP: The mTOR protein as a target in thyroid cancer. Expert Opin Ther Targets. 15:1099–1112. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Petrulea MS, Plantinga TS, Smit JW, Georgescu CE and Netea-Maier RT: PI3K/Akt/mTOR: A promising therapeutic target for non-medullary thyroid carcinoma. Cancer Treat Rev. 41:707–713. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Prabahar A and Natarajan J: MicroRNA mediated network motifs in autoimmune diseases and its crosstalk between genes, functions and pathways. J Immunol Methods. 440:19–26. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Otsu H, Watanabe M, Inoue N, Masutani R and Iwatani Y: Intraindividual variation of microRNA expression levels in plasma and peripheral blood mononuclear cells and the associations of these levels with the pathogenesis of autoimmune thyroid diseases. Clin Chem Lab Med. 55:626–635. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Li H and Ding G: Elevated serum inflammatory cytokines in lupus nephritis patients, in association with promoted hsa-miR-125a. Clin Lab. 62:631–638. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Hu H, Hu S, Xu S, Gao Y, Zeng F and Shui H: miR-29b regulates Ang II-induced EMT of rat renal tubular epithelial cells via targeting PI3K/AKT signaling pathway. Int J Mol Med. 42:453–460. 2018.PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Xia N, Chen G, Liu M, Ye X, Pan Y, Ge J, Mao Y, Wang H, Wang J and Xie S: Anti-inflammatory effects of luteolin on experimental autoimmune thyroiditis in mice. Exp Ther Med. 12:4049–4054. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Buschur E, Sarma AV, Pietropaolo M, Dunn RL, Braffett BH, Cleary PA, Cowie C, Larkin ME, Wessells H, Nathan DM, et al: Self-reported autoimmune disease by sex in the diabetes control and complications trial/epidemiology of diabetes interventions and complications (DCCT/EDIC) study. Diabetes Care. 37:e28–29. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Höfling DB, Chavantes MC, Juliano AG, Cerri GG, Knobel M, Yoshimura EM and Chammas MC: Low-level laser in the treatment of patients with hypothyroidism induced by chronic autoimmune thyroiditis: A randomized, placebo-controlled clinical trial. Lasers Med Sci. 28:743–753. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Lee J, Yi S, Kang YE, Chang JY, Kim JT, Sul HJ, Kim JO, Kim JM, Kim J, Porcelli AM, et al: Defective ciliogenesis in thyroid hürthle cell tumors is associated with increased autophagy. Oncotarget. 7:79117–79130. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Xu C, Wu F, Mao C, Wang X, Zheng T, Bu L, Mou X, Zhou Y, Yuan G, Wang S and Xiao Y: Excess iodine promotes apoptosis of thyroid follicular epithelial cells by inducing autophagy suppression and is associated with Hashimoto thyroiditis disease. J Autoimmun. 75:50–57. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Kim JK, Yuk JM, Kim SY, Kim TS, Jin HS, Yang CS and Jo EK: MicroRNA-125a inhibits autophagy activation and antimicrobial responses during mycobacterial infection. J Immunol. 194:5355–5365. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Raad H, Eskalli Z, Corvilain B, Miot F and De Deken X: Thyroid hydrogen peroxide production is enhanced by the Th2 cytokines, IL-4 and IL-13, through increased expression of the dual oxidase 2 and its maturation factor DUOXA2. Free Radic Biol Med. 56:216–225. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Koc A, Batar B, Celik O, Onaran I, Tasan E and Sultuybek GK: Polymorphism of the NFKB1 affects the serum inflammatory levels of IL-6 in Hashimoto thyroiditis in a Turkish population. Immunobiology. 219:531–536. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Xian H, Wang F, Teng W, Yang D and Zhang M: Thyroid hormone induce a p53-dependent DNA damage through PI3K/Akt activation in sperm. Gene. 615:1–7. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Zhu P, Liao LY, Zhao TT, Mo XM, Chen GG and Liu ZM: GPER/ERK&AKT/NF-κB pathway is involved in cadmium-induced proliferation, invasion and migration of GPER-positive thyroid cancer cells. Mol Cell Endocrinol. 442:68–80. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Tang H, Li RP, Liang P, Zhou YL and Wang GW: miR-125a inhibits the migration and invasion of liver cancer cells via suppression of the PI3K/AKT/mTOR signaling pathway. Oncol Lett. 10:681–686. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen D, Huang X, Lu S, Deng H, Gan H, Huang R and Zhang B: miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway. Exp Ther Med 17: 2465-2472, 2019.
APA
Chen, D., Huang, X., Lu, S., Deng, H., Gan, H., Huang, R., & Zhang, B. (2019). miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway. Experimental and Therapeutic Medicine, 17, 2465-2472. https://doi.org/10.3892/etm.2019.7256
MLA
Chen, D., Huang, X., Lu, S., Deng, H., Gan, H., Huang, R., Zhang, B."miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway". Experimental and Therapeutic Medicine 17.4 (2019): 2465-2472.
Chicago
Chen, D., Huang, X., Lu, S., Deng, H., Gan, H., Huang, R., Zhang, B."miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2465-2472. https://doi.org/10.3892/etm.2019.7256
Copy and paste a formatted citation
x
Spandidos Publications style
Chen D, Huang X, Lu S, Deng H, Gan H, Huang R and Zhang B: miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway. Exp Ther Med 17: 2465-2472, 2019.
APA
Chen, D., Huang, X., Lu, S., Deng, H., Gan, H., Huang, R., & Zhang, B. (2019). miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway. Experimental and Therapeutic Medicine, 17, 2465-2472. https://doi.org/10.3892/etm.2019.7256
MLA
Chen, D., Huang, X., Lu, S., Deng, H., Gan, H., Huang, R., Zhang, B."miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway". Experimental and Therapeutic Medicine 17.4 (2019): 2465-2472.
Chicago
Chen, D., Huang, X., Lu, S., Deng, H., Gan, H., Huang, R., Zhang, B."miRNA‑125a modulates autophagy of thyroiditis through PI3K/Akt/mTOR signaling pathway". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2465-2472. https://doi.org/10.3892/etm.2019.7256
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team