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Effect of fecal microbiota transplantation on experimental colitis in mice

  • Authors:
    • Julan Zhou
    • Zhongyin Zhou
    • Panpan Ji
    • Min Ma
    • Jinkun Guo
    • Shujuan Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Renmin Hospital of Wuhan University, Hubei Key Laboratory of Digestive System Disease, Wuhan, Hubei 430060, P.R. China, Department of Gastroenterology, Zhengzhou Third People's Hospital, Zhengzhou, Henan 450000, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2581-2586
    |
    Published online on: February 13, 2019
       https://doi.org/10.3892/etm.2019.7263
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Abstract

The aim of the present study was to investigate the effect of fecal microbiota transplantation (FMT) on the acute inflammatory response in a murine model of dextran sulfate sodium (DSS)‑induced colitis, and to delineate the putative underlying mechanism(s). Mice were divided into four groups, namely the normal control, DSS, 5‑aminosalicylic acid (5‑ASA) and FMT group. Mice in the DSS, 5‑ASA and FMT groups were orally administered 3% DSS (w/v) solution for 7 days to induce colitis. On days 1, 3, 5 and 7, mice in the DSS, 5‑ASA and FMT groups were respectively administered 0.5% carboxymethylcellulose sodium, 5‑ASA suspension and fecal suspension by enema. The disease activity index of each mouse was calculated on a daily basis. All mice were sacrificed on day 8, and the length of their colons was measured. Myeloperoxidase (MPO) activity, and the levels of tumor necrosis factor α (TNF‑α), interleukin (IL)‑1β and IL‑10 in the colon tissues of each group were also measured. Compared with that in the DSS group, FMT ameliorated the severity of inflammation due to ulcerative colitis in mice, which was accompanied by a significantly decreased MPO activity, reduced levels of TNF‑α and IL‑1β, and an increased level of IL‑10 in colon tissue (all P<0.05). Taken together, these results demonstrated that FMT exerted a therapeutic effect on experimental colitis in mice, and the associated mechanism is likely to involve the remodeling of the intestinal flora and regulation of intestinal T‑cell immunity homeostasis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Zhou Z, Ji P, Ma M, Guo J and Jiang S: Effect of fecal microbiota transplantation on experimental colitis in mice. Exp Ther Med 17: 2581-2586, 2019.
APA
Zhou, J., Zhou, Z., Ji, P., Ma, M., Guo, J., & Jiang, S. (2019). Effect of fecal microbiota transplantation on experimental colitis in mice. Experimental and Therapeutic Medicine, 17, 2581-2586. https://doi.org/10.3892/etm.2019.7263
MLA
Zhou, J., Zhou, Z., Ji, P., Ma, M., Guo, J., Jiang, S."Effect of fecal microbiota transplantation on experimental colitis in mice". Experimental and Therapeutic Medicine 17.4 (2019): 2581-2586.
Chicago
Zhou, J., Zhou, Z., Ji, P., Ma, M., Guo, J., Jiang, S."Effect of fecal microbiota transplantation on experimental colitis in mice". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2581-2586. https://doi.org/10.3892/etm.2019.7263
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Zhou Z, Ji P, Ma M, Guo J and Jiang S: Effect of fecal microbiota transplantation on experimental colitis in mice. Exp Ther Med 17: 2581-2586, 2019.
APA
Zhou, J., Zhou, Z., Ji, P., Ma, M., Guo, J., & Jiang, S. (2019). Effect of fecal microbiota transplantation on experimental colitis in mice. Experimental and Therapeutic Medicine, 17, 2581-2586. https://doi.org/10.3892/etm.2019.7263
MLA
Zhou, J., Zhou, Z., Ji, P., Ma, M., Guo, J., Jiang, S."Effect of fecal microbiota transplantation on experimental colitis in mice". Experimental and Therapeutic Medicine 17.4 (2019): 2581-2586.
Chicago
Zhou, J., Zhou, Z., Ji, P., Ma, M., Guo, J., Jiang, S."Effect of fecal microbiota transplantation on experimental colitis in mice". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2581-2586. https://doi.org/10.3892/etm.2019.7263
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