Injured pancreatic β cells enhance the release of miR‑375‑3p into the extracellular space

Chiba,Mitsuru; Niiyama,Ikumi; Uehara,Haruka; Kuwata,Haruka

doi:10.3892/etm.2019.7276

Injured pancreatic β cells enhance the release of miR‑375‑3p into the extracellular space

Authors:
- Mitsuru Chiba
- Ikumi Niiyama
- Haruka Uehara
- Haruka Kuwata
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Affiliations: Department of Bioscience and Laboratory Medicine, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan, Department of Medical Technology, School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan
Published online on: February 13, 2019 https://doi.org/10.3892/etm.2019.7276
Pages: 2815-2820

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Abstract

miR-375-3p is a highly expressed microRNA in pancreatic β cells. We have previously reported that when mice were exposed to 7 Gy X‑ray irradiation, miR‑375‑3p was increased in the serum and there was cytotoxicity in pancreatic β cells. However, it was unknown whether miR‑375‑3p is then released from injured pancreatic β cells to the extracellular space. The present study investigated the effect of ionizing radiation and streptozotocin (STZ) treatment on the expression of extracellular miR‑375‑3p into culture supernatants using the rat pancreatic β cell line RIN‑5F. Cell growth was reduced, and cell death was increased at 24 h following exposure to 7 Gy irradiation as well as 24 h following treatment with 30 mM STZ compared with the control. Expression levels of miR‑375‑3p were significantly increased 24 h after 30 mM STZ treatment, yet this was only observed at 48 h following exposure to 7 Gy compared with the control. This suggests that the mechanism of cell death in RIN‑5F is different between 7 Gy irradiation and 30 mM STZ treatment. The results of the present study suggest that injured pancreatic β cells enhance the release of miR‑375‑3p from cells into extracellular space.

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