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A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads

  • Authors:
    • Yanli Xu
    • Di Xiao
    • Huifang Zhang
    • Lihua He
    • Yixin Gu
    • Xianhui Peng
    • Xiaohuan Gao
    • Zhijun Liu
    • Jianzhong Zhang
  • View Affiliations / Copyright

    Affiliations: Hebei University of Engineering, Affiliated Hospital, College of Medicine, Handan, Hebei 056002, P.R. China, State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China, Beijing Huawei Tongke Medical Research Center, Beijing 100069, P.R. China
  • Pages: 2995-3002
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    Published online on: February 22, 2019
       https://doi.org/10.3892/etm.2019.7293
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Abstract

In order to explore convenient and stable fatigue markers, we studied various high‑molecular‑weight peptide fragments under fatigue state and non‑fatigue state in the saliva using time of flight mass spectrometry. The saliva samples were collected from 10 healthy volunteers that were in the condition of fatigue and non‑fatigue, respectively. Moreover, the time of flight mass spectrometry was conducted using two kinds of sample treatment methods, the magnetic beads enrichment (MB) and direct detection of stock solution. This was followed by modeling via the mass spectra of MB and supernatant (stock solution) directly collected after centrifugation. Both MB and direct sampling produced good spectrograms between 1,000 and 15,000 Da, while some peaks were lost in the enrichment. The spectrograms in the early and late period were different in each individual. Due to the limited sample size, 20 early and 20 late spectrograms were used for modeling analysis. Three different peptides were identified in the stock solution samples that can be detected in both fatigue and non‑fatigue groups. The cross validity of MB model was 92.06%, while that of the stock solution model was 95.49%. The results showed that there were different peaks within the molecular weight of 2,000‑15,000 Da, which provided a scientific basis for further realization of the convenient fatigue detection method based on the biosensor technique, with important theoretical and practical significance.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Xiao D, Zhang H, He L, Gu Y, Peng X, Gao X, Liu Z and Zhang J: A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads. Exp Ther Med 17: 2995-3002, 2019.
APA
Xu, Y., Xiao, D., Zhang, H., He, L., Gu, Y., Peng, X. ... Zhang, J. (2019). A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads. Experimental and Therapeutic Medicine, 17, 2995-3002. https://doi.org/10.3892/etm.2019.7293
MLA
Xu, Y., Xiao, D., Zhang, H., He, L., Gu, Y., Peng, X., Gao, X., Liu, Z., Zhang, J."A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads". Experimental and Therapeutic Medicine 17.4 (2019): 2995-3002.
Chicago
Xu, Y., Xiao, D., Zhang, H., He, L., Gu, Y., Peng, X., Gao, X., Liu, Z., Zhang, J."A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2995-3002. https://doi.org/10.3892/etm.2019.7293
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Xiao D, Zhang H, He L, Gu Y, Peng X, Gao X, Liu Z and Zhang J: A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads. Exp Ther Med 17: 2995-3002, 2019.
APA
Xu, Y., Xiao, D., Zhang, H., He, L., Gu, Y., Peng, X. ... Zhang, J. (2019). A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads. Experimental and Therapeutic Medicine, 17, 2995-3002. https://doi.org/10.3892/etm.2019.7293
MLA
Xu, Y., Xiao, D., Zhang, H., He, L., Gu, Y., Peng, X., Gao, X., Liu, Z., Zhang, J."A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads". Experimental and Therapeutic Medicine 17.4 (2019): 2995-3002.
Chicago
Xu, Y., Xiao, D., Zhang, H., He, L., Gu, Y., Peng, X., Gao, X., Liu, Z., Zhang, J."A prospective study on peptide mapping of human fatigue saliva markers based on magnetic beads". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2995-3002. https://doi.org/10.3892/etm.2019.7293
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