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Article

Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors

  • Authors:
    • Faliang Dai
    • Shizhen Dong
    • Zhenhua Rong
    • Qingxia Xuan
    • Pan Chen
    • Menglu Chen
    • Yonggang Fan
    • Qiang Gao
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Hepatology, Beijing Rehabilitation Hospital, Capital Medical University, Beijing 100144, P.R. China, Department of Clinical Laboratory Diagnosis, The First Affiliated Hospital and Clinical Medical College, Henan University of Science and Technology, Luoyang, Henan 471023, P.R. China, Department of Minimally Invasive Interventional Oncology, Caoxian People's Hospital, Heze, Shandong 274400, P.R. China, Department of Gastroenterology and Hepatology, The First Affiliated Hospital and Clinical Medical College, Henan University of Science and Technology, Luoyang, Henan 471023, P.R. China, Department of Surgery, The First Affiliated Hospital and Clinical Medical College, Henan University of Science and Technology, Luoyang, Henan 471023, P.R. China
  • Pages: 3181-3188
    |
    Published online on: February 26, 2019
       https://doi.org/10.3892/etm.2019.7317
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Abstract

Inflammatory bowel disease (IBD) is a risk factor in colon cancer. Endoplasmic reticulum (ER) stress is associated with IBD and cancer. In the current study an azoxymethane (AOM) and dextran sulfate sodium (DSS)‑induced mouse colonic tumor model was established to analyze the expression of ER stress chaperone molecules. Female C57BL/6 mice were intraperitoneally injected with 12 mg/kg AOM. On the 7th day following AOM injection, mice were treated with 1% DSS supplemented to the drinking water for 7 days, then followed by 14 days of normal drinking water. The cycle of 7 days DSS plus 14 days normal water was repeated twice and colonic tumors were evaluated for their number and size. Mice in the control group were injected with saline and received normal drinking water for the course of the experiment. mRNA levels of cytokines, inositol‑requiring enzyme (IRE)1α and 1β, their downstream targets X‑box binding protein (XBP)1u, XBP1s and mucin (MUC) 2 and interleukin (IL)‑6, IL‑8 and tumor necrosis factor (TNF)‑α were detected by reverse transcription‑quantitative polymerase chain reaction. IRE1α, IRE1β and MUC2 protein expression was evaluated by immunohistochemistry, and IRE1α and IRE1β levels were further assessed by western blot analysis. It was observed that tumors developed in the distal colon of mice treated with AOM/DSS. IL‑6, IL‑8 and TNF‑α mRNA levels were significantly increased in mice of the tumor group compared with mice of the control group. There were no significant differences in IRE1α mRNA and protein expression between the two groups and XBP1s mRNA levels were increased in the tumor compared with the control group. IRE1β and MUC2 mRNA levels were significantly decreased in the tumor compared with the control group (decreased by 42 and 30%, respectively). IRE1β and MUC2 proteins were predominately expressed in colonic epithelial cells and expression was decreased in the tumor compared with the control group. In conclusion, the downregulation of IRE1β and MUC2 may reduce the ability of colon tissues to resist inflammation, thus promoting the occurrence and development of colonic tumors.
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Copy and paste a formatted citation
Spandidos Publications style
Dai F, Dong S, Rong Z, Xuan Q, Chen P, Chen M, Fan Y and Gao Q: Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors. Exp Ther Med 17: 3181-3188, 2019.
APA
Dai, F., Dong, S., Rong, Z., Xuan, Q., Chen, P., Chen, M. ... Gao, Q. (2019). Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors. Experimental and Therapeutic Medicine, 17, 3181-3188. https://doi.org/10.3892/etm.2019.7317
MLA
Dai, F., Dong, S., Rong, Z., Xuan, Q., Chen, P., Chen, M., Fan, Y., Gao, Q."Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors". Experimental and Therapeutic Medicine 17.4 (2019): 3181-3188.
Chicago
Dai, F., Dong, S., Rong, Z., Xuan, Q., Chen, P., Chen, M., Fan, Y., Gao, Q."Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors". Experimental and Therapeutic Medicine 17, no. 4 (2019): 3181-3188. https://doi.org/10.3892/etm.2019.7317
Copy and paste a formatted citation
x
Spandidos Publications style
Dai F, Dong S, Rong Z, Xuan Q, Chen P, Chen M, Fan Y and Gao Q: Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors. Exp Ther Med 17: 3181-3188, 2019.
APA
Dai, F., Dong, S., Rong, Z., Xuan, Q., Chen, P., Chen, M. ... Gao, Q. (2019). Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors. Experimental and Therapeutic Medicine, 17, 3181-3188. https://doi.org/10.3892/etm.2019.7317
MLA
Dai, F., Dong, S., Rong, Z., Xuan, Q., Chen, P., Chen, M., Fan, Y., Gao, Q."Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors". Experimental and Therapeutic Medicine 17.4 (2019): 3181-3188.
Chicago
Dai, F., Dong, S., Rong, Z., Xuan, Q., Chen, P., Chen, M., Fan, Y., Gao, Q."Expression of inositol‑requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium‑induced mouse colonic tumors". Experimental and Therapeutic Medicine 17, no. 4 (2019): 3181-3188. https://doi.org/10.3892/etm.2019.7317
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