Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
May-2019 Volume 17 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2019 Volume 17 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2

  • Authors:
    • Jing Tang
    • Siwei Zhou
    • Fanghua Zhou
    • Xiumei Wen
  • View Affiliations / Copyright

    Affiliations: Orthopaedics Rehabilitation Center, Zhejiang Rehabilitation Medical Center, Hangzhou, Zhejiang 310051, P.R. China, Department of Emergency, The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3299-3306
    |
    Published online on: March 12, 2019
       https://doi.org/10.3892/etm.2019.7371
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The current study aimed to investigate the inhibitory effect of tanshinone IIA (Tan IIA) on the inflammatory response in patients with rheumatoid arthritis (RA) and explore its mechanism. A total of 50 patients with RA were randomly separated into the control group (15 cases) and the research group (35 cases). The tumor necrosis factor (TNF)‑α and interleukin (IL)‑6 levels in serum were determined, and peripheral blood mononuclear cells (PBMCs) were separated from patients with RA and cultured in vitro. The effects of the β‑arrestin 2 small interfering (si)RNA incubation, lipopolysaccharide (LPS) stimulation or Tan IIA incubation on TNF‑α and IL‑6 levels, and the expression levels of β‑arrestin 2, NAD‑dependent protein deacetylase sirtuin‑1 (SIRT1) and transcription factor p65 (p65) proteins were investigated. Prior to treatment, no significant differences in TNF‑α and IL‑6 levels in serum of patients with RA were identified between the research and control groups. Following treatment, the TNF‑α and IL‑6 levels in the serum of patients with RA in the research group were significantly lower compared with those in the research group prior to treatment and those in the control group following treatment (P<0.05). Tan IIA inhibited the LPS‑induced secretion of TNF‑α and IL‑6, upregulated the LPS‑inhibited expression of the β‑arrestin 2 and SIRT1 proteins, and downregulated the LPS‑induced expression of the p65 protein in the PBMCs of patients with RA. The β‑arrestin 2 small interfering (si)RNA significantly upregulated the secretion of TNF‑α and IL‑6, inhibited the expression of the SIRT1 protein and upregulated the expression of the p65 protein in PBMCs of patients with RA. Tan II A effectively increased the weight of rats with rheumatoid arthritis, and reduced the circumference of the left posterior ankle, the posterior plantar metatarsal thickness, and the content of serum TNF‑α and IL‑6. Tan IIA did not significantly reverse these β‑arrestin 2 siRNA‑induced changes. Tan IIA inhibited the inflammatory response in PBMCs of patients with RA by upregulating β‑arrestin 2 expression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Astry B, Harberts E and Moudgil KD: A cytokine-centric view of the pathogenesis and treatment of autoimmune arthritis. J Interferon Cytokine Res Official. 31:927–940. 2011. View Article : Google Scholar

2 

Mcinnes IB and Schett G: Cytokines in the pathogenesis of rheumatoid arthritis. Nat Rev Immunol. 7:429–442. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Kunz M and Ibrahim SM: Cytokines and cytokine profiles in human autoimmune diseases and animal models of autoimmunity. Mediators Inflamm. 2009:9792582009. View Article : Google Scholar : PubMed/NCBI

4 

Simsek I: TNF inhibitors for rheumatoid arthritis-a year in review. Bull NYU Hosp Jt Dis. 69:220–224. 2011.PubMed/NCBI

5 

Woodrick RS and Ruderman EM: Interleukin 6 inhibition-RA and beyond. Bull NYU Hosp Jt Dis. 69:225–229. 2011.PubMed/NCBI

6 

Kremer JM, Westhovens R, Leon M, Di Giorgio E, Alten R, Steinfeld S, Russell A, Dougados M, Emery P, Nuamah IF, et al: Treatment of rheumatoid arthritis by selective inhibition of T-cell activation with fusion protein CTLA4Ig. N Engl J Med. 349:1907–1915. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Rao DA, Gurish MF, Marshall JL, Slowikowski K, Fonseka CY, Liu Y, Donlin LT, Henderson LA, Wei K, Mizoguchi F, et al: Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis. Nature. 542:110–114. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Liu C, Wang D, Lu S, Xu Q, Zhao L, Zhao J, Song Y and Wang H: Increased circulating follicular treg cells are associated with lower levels of autoantibodies in patients with rheumatoid arthritis in stable remission. Arthritis Rheumatol. 70:711–721. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Yu Q, Chen H, Sheng L, Liang Y and Li Q: Sodium tanshinone IIA sulfonate prolongs the survival of skin allografts by inhibiting inflammatory cell infiltration and T cell proliferation. Int immunopharmacol. 22:277–284. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Shang Q, Wang H, Li S and Xu H: The effect of sodium tanshinone IIA sulfate and simvastatin on elevated serum levels of inflammatory markers in patients with coronary heart disease: A study protocol for a randomized controlled trial. Evid Based Complement Alternat Med. 2013:7565192013. View Article : Google Scholar : PubMed/NCBI

11 

Jie L, Du H, Huang Q, Wei S, Huang R and Sun W: Tanshinone IIA induces apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis via blockade of the cell cycle in the G2/M phase and a mitochondrial pathway. Biol Pharm Bull. 37:1366–1372. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Kay J and Upchurch KS: ACR/EULAR 2010 rheumatoid arthritis classification criteria. Rheumatology (Oxford):. 51 (Suppl 6):vi5–vi9. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Nizamutdinova IT, Kim YM, Jin H, Son KH, Lee JH, Chang KC and Kim HJ: Tanshinone IIA inhibits TNF-α-mediated induction of VCAM-1 but not ICAM-1 through the regulation of GATA-6 and IRF-1. Int Immunopharmacol. 14:650–657. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Lin C, Wang L, Wang H, Yang L, Guo H and Wang X: Tanshinone IIA inhibits breast cancer stem cells growth in vitro and in vivo through attenuation of IL-6/STAT3/NF-κB signaling pathways. J Cell Biochem. 114:2061–2070. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Li P, Cook JA, Gilkeson GS, Luttrell LM, Wang L, Borg KT, Halushka PV and Fan H: Increased expression of beta-arrestin 1 and 2 in murine models of rheumatoid arthritis: Isoform specific regulation of inflammation. Mol Immunol. 49:64–74. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Lan W, Petznick A, Heryati S, Rifada M and Tong L: Nuclear factor-κB: Central regulator in ocular surface inflammation and diseases. Ocul Surf. 10:137–148. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Zheng C, Yin Q and Wu H: Structural studies of NF-κB signaling. Cell Res. 21:183–195. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Thakur BK, Chandra A, Dittrich T, Welte K and Chandra P: Inhibition of SIRT1 by HIV-1 viral protein Tat results in activation of p53 pathway. Biochem Biophys Res Commun. 424:245–250. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Zhao W, Kruse JP, Tang Y, Jung SY, Qin J and Gu W: Negative regulation of the deacetylase SIRT1 by DBC1. Nature. 451:587–590. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Kuno A, Hori YS, Hosoda R, Tanno M, Miura T, Shimamoto K and Horio Y: Resveratrol improves cardiomyopathy in dystrophin-deficient mice through SIRT1 protein-mediated modulation of p300 protein. J Biol Chem. 288:5963–5972. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Zelová H and Hošek J: TNF-α signalling and inflammation: Interactions between old acquaintances. Inflamm Res. 62:641–651. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Neurath MF and Finotto S: IL-6 signaling in autoimmunity, chronic inflammation and inflammation-associated cancer. Cytokine Growth Factor Rev. 22:83–89. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Seriolo B, Paolino S, Sulli A, Fasciolo D and Cutolo M: Effects of anti-TNF-alpha treatment on lipid profile in patients with active rheumatoid arthritis. Ann NY Acad Sci. 1069:414–419. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Nishimoto N, Hashimoto J, Miyasaka N, Yamamoto K, Kawai S, Takeuchi T, Murata N, van der Heijde D and Kishimoto T: Study of active controlled monotherapy used for rheumatoid arthritis, an IL-6 inhibitor (SAMURAI): Evidence of clinical and radiographic benefit from an × ray reader-blinded randomised controlled trial of tocilizumab. Ann Rheum Dis. 66:1162–1167. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tang J, Zhou S, Zhou F and Wen X: Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2. Exp Ther Med 17: 3299-3306, 2019.
APA
Tang, J., Zhou, S., Zhou, F., & Wen, X. (2019). Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2. Experimental and Therapeutic Medicine, 17, 3299-3306. https://doi.org/10.3892/etm.2019.7371
MLA
Tang, J., Zhou, S., Zhou, F., Wen, X."Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2". Experimental and Therapeutic Medicine 17.5 (2019): 3299-3306.
Chicago
Tang, J., Zhou, S., Zhou, F., Wen, X."Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3299-3306. https://doi.org/10.3892/etm.2019.7371
Copy and paste a formatted citation
x
Spandidos Publications style
Tang J, Zhou S, Zhou F and Wen X: Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2. Exp Ther Med 17: 3299-3306, 2019.
APA
Tang, J., Zhou, S., Zhou, F., & Wen, X. (2019). Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2. Experimental and Therapeutic Medicine, 17, 3299-3306. https://doi.org/10.3892/etm.2019.7371
MLA
Tang, J., Zhou, S., Zhou, F., Wen, X."Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2". Experimental and Therapeutic Medicine 17.5 (2019): 3299-3306.
Chicago
Tang, J., Zhou, S., Zhou, F., Wen, X."Inhibitory effect of tanshinone IIA on inflammatory response in rheumatoid arthritis through regulating β‑arrestin 2". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3299-3306. https://doi.org/10.3892/etm.2019.7371
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team