Effect of β‑patchoulene on cerebral ischemia‑reperfusion injury and the TLR4/NF‑κB signaling pathway

Zhang,Fu‑Bo; Wang,Jian‑Ping; Zhang,Hong‑Xia; Fan,Gui‑Mei; Cui,Xin

doi:10.3892/etm.2019.7374

Effect of β‑patchoulene on cerebral ischemia‑reperfusion injury and the TLR4/NF‑κB signaling pathway

Authors:
- Fu‑Bo Zhang
- Jian‑Ping Wang
- Hong‑Xia Zhang
- Gui‑Mei Fan
- Xin Cui
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Affiliations: Department of Neurology, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China, Department of Rheumatology, Cangzhou Central Hospital, Cangzhou, Hebei 061001, P.R. China
Published online on: March 12, 2019 https://doi.org/10.3892/etm.2019.7374
Pages: 3335-3342
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

β‑patchoulene (β‑PAE), an active constituent of the Pogostemon cablin, is well known for its anti‑inﬂammatory and antioxidative functions in various diseases. However, little is known about the impact of β‑PAE on the cerebral ischemia‑reperfusion (I/R) injury. The current study aimed to determine the neuroprotective effect of β‑PAE and the underlying mechanisms on cerebral I/R injury. Following pretreatment with β‑PAE (10 mg/kg body weight) by tail intravenous injection for 1 h, Sprague‑Dawley rats were subjected to middle cerebral artery occlusion for 2 h and reperfusion for 24 h. The results indicated that pretreatment with β‑PAE could diminish the infarct volume, decrease the brain water content, reduce the neurological deficit score and restore the mitochondrial membrane potential, compared with the untreated I/R injury group. Furthermore, cell apoptosis was markedly suppressed by β‑PAE, and this effect was associated with the decreased apoptosis regulator BAX/apoptosis regulator Bcl‑2 expression ratio and caspase‑3 activity. In addition, β‑PAE significantly inhibited the release of proinflammatory factors, including tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6. Superoxide generation and malondialdehyde levels were reduced while the levels of glutathione peroxidase and superoxide dismutase were elevated following treatment with β‑PAE, indicating the antioxidative role of β‑PAE in cerebral I/R injury. Furthermore, the Toll‑like receptor 4 (TLR4)/nuclear factor‑κB (NF‑κB) signaling pathway was inhibited by β‑PAE, as demonstrated by the decreased TLR4 expression and nuclear translocation of p65, and increased IκBα level. Taken together, the results suggested that β‑PAE may exhibit a neuroprotective effect on cerebral I/R injury in rats through inactivating the TLR4/NF‑κB signaling pathway.

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