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Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury

  • Authors:
    • Hu Batulu
    • Guo‑Jia Du
    • Da‑Zhi Li
    • Duishanbai Sailike
    • Yu‑Hua Fan
    • Dangmurenjiafu Geng
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China, Department of Neurosurgery, Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China, Department of Nutrition Section, The People's Hospital of Xinjiang Bortala Autonomous Prefecture of Monglia, Bortala, Xinjiang 833400, P.R. China
    Copyright: © Batulu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4109-4115
    |
    Published online on: March 20, 2019
       https://doi.org/10.3892/etm.2019.7423
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Abstract

The present study assessed the effects of poly‑arginine R18 and its promotion of neurocyte cell growth via autophagy in traumatic brain injury (TBI), and aimed to determine the possible mechanism by which this occurs. Brain water content was measured to analyze the effects of poly‑arginine R18 in TBI. MTT and lactate dehydrogenase activity assays were performed to measure N2A cell growth. Western blotting and immunofluorescence staining were also performed to determine the protein expression of Bcl‑2 associated X, LC3, Beclin‑1 and p62. The results demonstrated that poly‑arginine R18 treatment reduced neurocyte apoptosis and promoted neurocyte cell growth via the activation of autophagy in a rat model of TBI. Furthermore, poly‑arginine R18 treatment promoted neurocyte cell growth, reduced apoptosis, induced the protein expression of LC3 and Beclin‑1, and suppressed p62 expression by promoting autophagy in vitro. In addition, the inhibition of autophagy attenuated the effects of poly‑arginine R18 on cell growth in vitro. Collectively, the results demonstrate the effects of poly‑arginine R18 on neurocyte cell growth via autophagy activation in a model of TBI, and poly‑arginine R18 is therefore a potential therapeutic target in TBI.
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Copy and paste a formatted citation
Spandidos Publications style
Batulu H, Du GJ, Li DZ, Sailike D, Fan YH and Geng D: Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury. Exp Ther Med 17: 4109-4115, 2019.
APA
Batulu, H., Du, G., Li, D., Sailike, D., Fan, Y., & Geng, D. (2019). Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury. Experimental and Therapeutic Medicine, 17, 4109-4115. https://doi.org/10.3892/etm.2019.7423
MLA
Batulu, H., Du, G., Li, D., Sailike, D., Fan, Y., Geng, D."Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury". Experimental and Therapeutic Medicine 17.5 (2019): 4109-4115.
Chicago
Batulu, H., Du, G., Li, D., Sailike, D., Fan, Y., Geng, D."Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4109-4115. https://doi.org/10.3892/etm.2019.7423
Copy and paste a formatted citation
x
Spandidos Publications style
Batulu H, Du GJ, Li DZ, Sailike D, Fan YH and Geng D: Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury. Exp Ther Med 17: 4109-4115, 2019.
APA
Batulu, H., Du, G., Li, D., Sailike, D., Fan, Y., & Geng, D. (2019). Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury. Experimental and Therapeutic Medicine, 17, 4109-4115. https://doi.org/10.3892/etm.2019.7423
MLA
Batulu, H., Du, G., Li, D., Sailike, D., Fan, Y., Geng, D."Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury". Experimental and Therapeutic Medicine 17.5 (2019): 4109-4115.
Chicago
Batulu, H., Du, G., Li, D., Sailike, D., Fan, Y., Geng, D."Effect of poly‑arginine R18 on neurocyte cell growth via autophagy in traumatic brain injury". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4109-4115. https://doi.org/10.3892/etm.2019.7423
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