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Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling

  • Authors:
    • Yang Zou
    • Weifeng Hu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China
    Copyright: © Zou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3999-4006
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    Published online on: March 20, 2019
       https://doi.org/10.3892/etm.2019.7425
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Abstract

Rheumatoid arthritis (RA) is a common systemic autoimmune disease mainly involving the formation of a synovial pannus, for which no effective treatment is available. In order to study the molecular biological mechanisms underlying the inhibition of RA synovial pannus by triptolide, differentially expressed genes in synovial tissues from an adjuvant arthritis (AA) rat model with and without triptolide treatment were detected in an mRNA microarray profile produced by Agilent Technologies and verified by reverse transcription‑quantitative polymerase chain reaction analysis (RT‑qPCR). An AA model was established by subcutaneously injecting 0.1 ml Freund's complete adjuvant daily for 18 days and scored by arthritis index assessment. Subsequently, triptolide (0.4 mg/kg) or an equivalent amount of saline was administered daily for 14 days. At the end of the experiment, synovial tissues were obtained from the ankle joints of the rats' hind legs. Total RNA was extracted and purified, and microarray hybridization was used to obtain the gene expression profile for RA with and without triptolide treatment. A total of 48 genes were identified to be differentially expressed between the treatment and model groups, including 32 upregulated and 16 downregulated genes. The possible signaling pathways associated with the effect of triptolide were investigated by Gene Ontology and pathway analysis, revealing that the phosphoinositide‑3 kinase (PI3K)/AKT signaling pathway has a key role in the proliferation and apoptosis of synovial cells in RA joints. Reverse transcription‑quantitative polymerase chain reaction analysis was applied to confirm the aberrant expression of key mRNAs and revealed that vascular endothelial growth factor (VEGF) A and C1q and tumor necrosis factor related protein 3 (C1QTNF3) were downregulated in the treatment group compared with the model group (P<0.05). In conclusion, triptolide may exert its effects against RA via the PI3K/AKT pathway and has an inhibitory effect on the expression of VEGFA and C1QTNF3, thus are potentially associated with the occurrence and development of RA.
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Copy and paste a formatted citation
Spandidos Publications style
Zou Y and Hu W: Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling. Exp Ther Med 17: 3999-4006, 2019.
APA
Zou, Y., & Hu, W. (2019). Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling. Experimental and Therapeutic Medicine, 17, 3999-4006. https://doi.org/10.3892/etm.2019.7425
MLA
Zou, Y., Hu, W."Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling". Experimental and Therapeutic Medicine 17.5 (2019): 3999-4006.
Chicago
Zou, Y., Hu, W."Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3999-4006. https://doi.org/10.3892/etm.2019.7425
Copy and paste a formatted citation
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Spandidos Publications style
Zou Y and Hu W: Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling. Exp Ther Med 17: 3999-4006, 2019.
APA
Zou, Y., & Hu, W. (2019). Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling. Experimental and Therapeutic Medicine, 17, 3999-4006. https://doi.org/10.3892/etm.2019.7425
MLA
Zou, Y., Hu, W."Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling". Experimental and Therapeutic Medicine 17.5 (2019): 3999-4006.
Chicago
Zou, Y., Hu, W."Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K‑AKT signaling". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3999-4006. https://doi.org/10.3892/etm.2019.7425
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