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MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression

  • Authors:
    • Min Xue
    • Jinhu Shen
    • Jie Cui
    • Jinxia Wu
    • Weili Qiao
    • Ningding Ding
    • Chengjie Song
    • Bin Shan
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Xuzhou Medical University, Xuzhou, Jiangsu 22100, P.R. China, Department of Orthopedics, Xuzhou City Hospital of Traditional Chinese Medicine, Xuzhou, Jiangsu, 221004, P.R. China, College of Medical Sciences, Washington State University, Spokane, WA 99201, USA
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3899-3906
    |
    Published online on: March 22, 2019
       https://doi.org/10.3892/etm.2019.7429
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Abstract

The present study aimed to investigate the function and mechanism of microRNA‑638 (miR‑638) in osteosarcoma. MiR‑638 expression change in patients with osteosarcoma was detected by reverse transcription‑quantitative polymerase chain reaction. Expression of miR‑638 was observed to be downregulated in patients with osteosarcoma compared with the control group. In vitro, overexpression of miR‑638 induced apoptosis, and inhibited cell proliferation and invasion of osteosarcoma cells. Overexpression of miR‑638 induced Bcl‑2 associated X and caspase‑3 protein expression, and suppressed cyclin D1, phospholipase D1 (PLD1) and vascular endothelial growth factor (VEGF) protein expression in osteosarcoma. The promotion of PLD1 decreased the effects of miR‑638 on osteosarcoma cell proliferation. In summary, it was demonstrated that miRNA‑638 expression change in patients with osteosarcoma and an in vitro model via PLD1 and VEGF expression and miRNA‑638 may be potential clinical indicators of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Xue M, Shen J, Cui J, Wu J, Qiao W, Ding N, Song C and Shan B: MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression. Exp Ther Med 17: 3899-3906, 2019.
APA
Xue, M., Shen, J., Cui, J., Wu, J., Qiao, W., Ding, N. ... Shan, B. (2019). MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression. Experimental and Therapeutic Medicine, 17, 3899-3906. https://doi.org/10.3892/etm.2019.7429
MLA
Xue, M., Shen, J., Cui, J., Wu, J., Qiao, W., Ding, N., Song, C., Shan, B."MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression". Experimental and Therapeutic Medicine 17.5 (2019): 3899-3906.
Chicago
Xue, M., Shen, J., Cui, J., Wu, J., Qiao, W., Ding, N., Song, C., Shan, B."MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3899-3906. https://doi.org/10.3892/etm.2019.7429
Copy and paste a formatted citation
x
Spandidos Publications style
Xue M, Shen J, Cui J, Wu J, Qiao W, Ding N, Song C and Shan B: MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression. Exp Ther Med 17: 3899-3906, 2019.
APA
Xue, M., Shen, J., Cui, J., Wu, J., Qiao, W., Ding, N. ... Shan, B. (2019). MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression. Experimental and Therapeutic Medicine, 17, 3899-3906. https://doi.org/10.3892/etm.2019.7429
MLA
Xue, M., Shen, J., Cui, J., Wu, J., Qiao, W., Ding, N., Song, C., Shan, B."MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression". Experimental and Therapeutic Medicine 17.5 (2019): 3899-3906.
Chicago
Xue, M., Shen, J., Cui, J., Wu, J., Qiao, W., Ding, N., Song, C., Shan, B."MicroRNA‑638 expression change in osteosarcoma patients via PLD1 and VEGF expression". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3899-3906. https://doi.org/10.3892/etm.2019.7429
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