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Article

Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus

  • Authors:
    • Shaobing Wang
    • Jun Li
    • Chenwen Zhang
    • Guiqing Xu
    • Zonghao Tang
    • Zhenghong Zhang
    • Yiping Liu
    • Zhengchao Wang
  • View Affiliations / Copyright

    Affiliations: Laboratory of Sport Physiology and Biomedicine, School of Physical Education and Sport Sciences, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China, Provincial Key Laboratory for Developmental Biology and Neurosciences, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, P.R. China
  • Pages: 4205-4212
    |
    Published online on: March 26, 2019
       https://doi.org/10.3892/etm.2019.7437
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Abstract

Previous studies by our group have indicated that exercise intervention can ameliorate endothelial dysfunction, which is an early pathophysiological change of prediabetes mellitus. The present study aimed to test the hypothesis that nitric oxide synthases (NOSs), which are expressed in blood vessel endothelium, contribute to the mitigation of vascular endothelium‑dependent dysfunction by aerobic exercise in prediabetes mellitus. A prediabetic rat model was established by feeding the rats an additional high‑energy diet, and was confirmed by testing blood glucose levels, the area‑under‑the‑curve for the blood glucose tests (P<0.05) and the changes to the histological morphology of the thoracic aorta. Further examination identified that NOS expression changed significantly between the control and prediabetes groups, indicating endothelial dysfunction in the prediabetic rats. Following aerobic exercise, a significant increase in NOS, endothelial (eNOS) mRNA and protein expression (P<0.05), and a significant decrease in NOS, inducible (iNOS) mRNA and protein expression (P<0.05) was identified in the prediabetic rats compared with the control group. No significant change in nitric oxide synthase, brain expression was observed in the prediabetic rat group compared with the control group. Notably, there was also a significant increase and decrease in eNOS and iNOS activity, respectively, in the prediabetes group compared with the control group (P<0.05). Furthermore, nitric oxide (NO) concentration in the vascular endothelium was detected, which revealed a significant increase in NO concentration in the prediabetic rats following aerobic exercise compared with the control (P<0.05). The present study provided results that demonstrated that aerobic exercise ameliorated the vascular endothelium‑dependent dysfunction through the NOS/NO signaling pathway, which is primarily regulated by NOS expression and activity, in prediabetes mellitus. The current study provided the theoretical basis for the use of exercise intervention to prevent diabetes mellitus during the early stage.
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1 

Tabák AG, Herder C, Rathmann W, Brunner EJ and Kivimäki M: Prediabetes: A high-risk state for diabetes development. Lancet. 379:2279–2290. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Nathan DM, Davidson MB, DeFronzo RA, Heine RJ, Henry RR, Pratley R, Zinman B and American Diabetes Association: Impaired fasting glucose and impaired glucose tolerance: Implications for care. Diabetes Care. 30:753–759. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Gupta A, Al-Aubaidy HA and Mohammed BI: Glucose dependent insulinotropic polypeptide and dipeptidyl peptidase inhibitors: Their roles in management of type 2 diabetes mellitus. Diabetes Metab Syndr 10 (2 Suppl 1). S170–S175. 2016. View Article : Google Scholar

4 

Liu Y, Li J, Zhang Z, Tang Y, Chen Z and Wang Z: Endocrinological analysis of endothelium-dependent vasodilation in middle-aged patients with impaired glucose tolerance during prediabetes mellitus. Exp Ther Med. 7:697–702. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Liu Y, Li J, Zhang Z, Tang Y, Chen Z and Wang Z: Effects of exercise intervention on vascular endothelium functions of patients with impaired glucose tolerance during prediabetes mellitus. Exp Ther Med. 5:1559–1565. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Fiorentino TV, Prioletta A, Zuo P and Folli F: Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases. Curr Pharm Des. 19:5695–5703. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Sena CM, Pereira AM and Seiça R: Endothelial dysfunction-a major mediator of diabetic vascular disease. Biochim Biophys Acta. 1832:2216–2231. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Fowler MJ and Michael J: Microvascular and macrovascular complications of diabetes. Clin Diabetes. 26:77–82. 2008. View Article : Google Scholar

9 

De Vriese AS, Verbeuren TJ, Van de Voorde J, Lameire NH and Vanhoutte PM: Endothelial dysfunction in diabetes. Br J Pharmacol. 130:963–974. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Chakraphan D, Sridulyakul P, Thipakorn B, Bunnag S, Huxley VH and Patumraj S: Attenuation of endothelial dysfunction by exercise training in STZ-induced diabetic rats. Clin Hemorheol Microcirc. 32:217–226. 2005.PubMed/NCBI

11 

Chis IC, Coseriu A, Simedrea R, Oros A, Nagy AL and Clichici S: In vivo effects of quercetin in association with moderate exercise training in improving streptozotocin-induced aortic tissue injuries. Molecules. 20:21770–21786. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Xie W, Parker JL and Heaps CL: Effect of exercise training on nitric oxide and superoxide/H2O2 signaling pathways in collateral-dependent porcine coronary arterioles. J Appl Physiol (1985). 112:1546–1555. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Zhang W, Wei QW, Wang ZC, Ding W, Wang W and Shi FX: Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats. J Zhejiang Univ Sci B. 12:55–64. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Zheng K, Sulieman FJ, Li J, Wei Q, Xu M and Shi F: Nitric oxide and thyroid hormone receptor alpha 1 contribute to ovarian follicular development in immature hyper- and hypo-thyroid rats. Reprod Biol. 15:27–33. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Xu M, Wei Q, Zheng K, Mao D, Zheng Y, Li Y and Shi F: Protective effects of Big-leaf mulberry and physiological roles of nitric oxide synthases in the testis of mice following water immersion and restraint stress. Acta Histochem. 116:1323–1330. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Laughlin MH, Pollock JS, Amann JF, Hollis ML, Woodman CR and Price EM: Training induces nonuniform increases in eNOS content along the coronary arterial tree. J Appl Physiol (1985). 90:501–510. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Sessa WC, Pritchard K, Seyedi N, Wang J and Hintze TH: Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression. Circ Res. 74:349–353. 1994. View Article : Google Scholar : PubMed/NCBI

18 

Miyauchi T, Maeda S, Iemitsu M, Kobayashi T, Kumagai Y, Yamaguchi I and Matsuda M: Exercise causes a tissue-specific change of NO production in the kidney and lung. J Appl Physiol (1985). 94:60–68. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Tatchum-Talom R, Schulz R, McNeill JR and Khadour FH: Upregulation of neuronal nitric oxide synthase in skeletal muscle by swim training. Am J Physiol Heart Circ Physiol. 279:H1757–H1766. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Pellegrin M, Berthelot A, Houdayer C, Gaume V, Deckert V and Laurant P: New insights into the vascular mechanisms underlying the beneficial effect of swimming training on the endothelial vasodilator function in apolipoprotein E-deficient mice. Atherosclerosis. 190:35–42. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Pellegrin M, Miguet-Alfonsi C, Berthelot A, Mazzolai L and Laurant P: Long-term swimming exercise does not modulate the Akt-dependent endothelial nitric oxide synthase phosphorylation in healthy mice. Can J Physiol Pharmacol. 89:72–76. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Rato L, Duarte AI, Tomás GD, Santos MS, Moreira PI, Socorro S, Cavaco JE, Alves MG and Oliveira PF: Pre-diabetes alters testicular PGC1-α/SIRT3 axis modulating mitochondrial bioenergetics and oxidative stress. Biochim Biophys Acta. 1837:335–344. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Braga VA, Couto GK, Lazzarin MC, Rossoni LV and Medeiros A: Aerobic exercise training prevents the onset of endothelial dysfunction via increased nitric oxide bioavailability and reduced reactive oxygen species in an experimental model of menopause. PLoS One. 10:e01253882015. View Article : Google Scholar : PubMed/NCBI

24 

Wu Y, Xue L, Du W, Huang B, Tang C, Liu C, Qiu H and Jiang Q: Polydatin restores endothelium-dependent relaxation in rat aorta rings impaired by high glucose: A novel insight into the PPARβ-NO signaling pathway. PLoS One. 10:e01262492015. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Wang SB, Xing BS, Yi L, Wang W and Xu YX: Expression of Frizzled 2 in the mouse ovary during oestrous cycle. J Anim Physiol Anim Nutr (Berl). 94:437–445. 2010.PubMed/NCBI

27 

Westerterp KR: Perception, passive overfeeding and energy metabolism. Physiol Behav. 89:62–65. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Ignarro LJ: Nitric oxide as a unique signaling molecule in the vascular system: A historical overview. J Physiol Pharmacol. 53:503–514. 2002.PubMed/NCBI

29 

Gielen S, Adams V, Linke A, Erbs S, Möbius-Winkler S, Schubert A, Schuler G and Hambrecht R: Exercise training in chronic heart failure: Correlation between reduced local inflammation and improved oxidative capacity in the skeletal muscle. Eur J Cardiovasc Prev Rehabil. 12:393–400. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Li J, Zhang C, Xu G, Tang Z, Zhang Z, Liu Y and Wang Z: Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus. Exp Ther Med 17: 4205-4212, 2019.
APA
Wang, S., Li, J., Zhang, C., Xu, G., Tang, Z., Zhang, Z. ... Wang, Z. (2019). Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus. Experimental and Therapeutic Medicine, 17, 4205-4212. https://doi.org/10.3892/etm.2019.7437
MLA
Wang, S., Li, J., Zhang, C., Xu, G., Tang, Z., Zhang, Z., Liu, Y., Wang, Z."Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus". Experimental and Therapeutic Medicine 17.5 (2019): 4205-4212.
Chicago
Wang, S., Li, J., Zhang, C., Xu, G., Tang, Z., Zhang, Z., Liu, Y., Wang, Z."Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4205-4212. https://doi.org/10.3892/etm.2019.7437
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Li J, Zhang C, Xu G, Tang Z, Zhang Z, Liu Y and Wang Z: Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus. Exp Ther Med 17: 4205-4212, 2019.
APA
Wang, S., Li, J., Zhang, C., Xu, G., Tang, Z., Zhang, Z. ... Wang, Z. (2019). Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus. Experimental and Therapeutic Medicine, 17, 4205-4212. https://doi.org/10.3892/etm.2019.7437
MLA
Wang, S., Li, J., Zhang, C., Xu, G., Tang, Z., Zhang, Z., Liu, Y., Wang, Z."Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus". Experimental and Therapeutic Medicine 17.5 (2019): 4205-4212.
Chicago
Wang, S., Li, J., Zhang, C., Xu, G., Tang, Z., Zhang, Z., Liu, Y., Wang, Z."Effects of aerobic exercise on the expressions and activities of nitric oxide synthases in the blood vessel endothelium in prediabetes mellitus". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4205-4212. https://doi.org/10.3892/etm.2019.7437
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