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Article

Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening

  • Authors:
    • Lin Li
    • Zhengmei Sha
    • Yanyan Wang
    • Dongli Yang
    • Jinghao Li
    • Zhenzhen Duan
    • Hongbo Wang
    • Yuhong Li
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P.R. China, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P.R. China
  • Pages: 4643-4652
    |
    Published online on: April 2, 2019
       https://doi.org/10.3892/etm.2019.7462
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Abstract

Increasing evidence has indicated that opening of the mitochondrial permeability transition pore (mPTP) has a vital role in myocardial ischemia/reperfusion (I/R) injury. Shenmai injection (SMI) plus Danshen injection (DSI) combination, termed Yiqi Yangyin Huoxue (YYH) therapy is used in the clinic to treat cardiovascular diseases, including myocardial I/R injury. Previous studies by our group have demonstrated the protective effect of pretreatment with YYH against myocardial I/R injury in isolated rat hearts. The present study aimed to examine the protective effect of YYH against hypoxia/reoxygenation (H/R)‑ and H2O2‑induced cardiomyocyte injury, and to determine whether this effect is produced by inhibition of mPTP opening. Primary cardiomyocytes isolated from neonatal rats were cultured and randomly grouped into a control group, injury group and pretreatment group, with six duplicated wells in each group during each assay. Cardiomyocytes in the injury group were subjected to H/R to simulate I/R or exposed to H2O2 for 2 h to induce oxidative injury. Cellular injury was assessed via release of creatine kinase (CK) and lactate dehydrogenase (LDH), and cell viability was measured by an MTT assay. The mitochondrial membrane potential (ΔΨm) and cytosolic reactive oxygen species (ROS) were detected using the fluorescent probes rhodamine123 (Rh123) and chloromethyl‑2,7‑dichlorodihydrofluorescein diacetate (CM‑H2DCFDA), respectively. Intracellular Ca2+, mitochondrial Ca2+ and mPTP opening were measured using fluo‑4 acetoxymethyl (Fluo‑4/AM), rhodamine‑2 acetoxymethyl (Rhod‑2/AM) and calcein acetoxymethyl (Calcein/AM) probes, respectively. The results indicated that pretreatment with YYH enhanced cell viability, increased ΔΨm, reduced CK and LDH release, and decreased intracellular ROS and Ca2+, thus reducing cardiomyocyte injury induced by H/R or H2O2. LY294002, a specific phosphoinositide 3‑kinase (PI3K) inhibitor, and PD98059, a specific inhibitor of the extracellular signal‑regulated kinase 1/2 (Erk1/2) pathway, eliminated the protective effects of the combination therapy on cell viability and the change in the ΔΨm in cardiomyocytes. In conclusion, pre‑treatment with YYH has cardioprotective effects against H/R injury and oxidative stress via activation of the PI3K/Akt and Erk1/2 signaling pathways, which reduces mPTP opening, overproduction of ROS and calcium overload.
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View References

1 

Mathers CD and Loncar D: Projections of gobal mortality and burden of disease from 2002 to 2030. PLoS Med. 3:e4422006. View Article : Google Scholar : PubMed/NCBI

2 

Hausenloy DJ and Yellon DM: Myocardial ischemia-reperfusion injury: A neglected therapeutic target. J Clin Investig. 123:92–100. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Piot C, Croisille P, Staat P, Thibault H, Rioufol G, Mewton N, Elbelghiti R, Cung TT, Bonnefoy E, Angoulvant D, et al: Effect of cyclosporine on reperfusion injury in acute myocardial infarction. N Engl J Med. 359:473–481. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Ibanez B, Macaya C, Sánchez-Brunete V, Pizarro G, Fernández-Friera L, Mateos A, Fernández-Ortiz A, García-Ruiz JM, García-Álvarez A, Iñiguez A, et al: Effect of early metoprolol on infarct size in ST-segment-elevation myocardial infarction patients undergoing primary percutaneous coronary intervention: The effect of metoprolol in cardioprotection during an acute myocardial infarction (METOCARD-CNIC) trial. Circulation. 128:1495–1503. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Roolvink V, Rasoul S, Ottervanger JP, Dambrink JH, Lipsic E, van der Horst IC, de Smet B, Kedhi E, Marcel Gosselink AT, Piek JJ, et al: Rationale and design of a double-blind, multicenter, randomized, placebo-controlled clinical trial of early administration of intravenous β-blocker in patients with ST-elevation myocardial infarction before primary percutaneous coronary intervention: EARLY β-blocker administration before primary PCI in patients with ST-elevation myocardial infarction trial. Am Heart J. 168:661–666. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Murphy E and Steenbergen C: Mechanisms underlying acute protection from cardiac ischemia-reperfusion injury. Physiol Rev. 88:581–609. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Halestrap AP and Richardson AP: The mitochondrial permeability transition: A current perspective on its identity and role in ischaemia/reperfusion injury. J Mol Cell Cardiol. 78:129–141. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Ong SB, Samangouei P, Kalkhoran SB and Hausenloy DJ: The mitochondrial permeability transition pore and its role in myocardial ischemia reperfusion injury. J Mol Cell Cardiol. 78:23–34. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Hausenloy DJ, Ong SB and Yellon DM: The mitochondrial permeability transition pore as a target for preconditioning and postconditioning. Basic Res Cardiol. 104:189–202. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Li L, Zhou YF, Li YL, Wang LL, Aria H, Qi Y and Xu Y: Aqueous extract of Cortex Dictamni protects H9c2 cardiomyocytes from hypoxia/reoxygenation-induced oxidative stress and apoptosis by PI3K/Akt signaling pathway. Biomed Pharmacother. 89:233–244. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Yu J, Gu LQ, Xin YF, Gao HY, Xu XZ, Zhuang S, Zhou GL, You ZQ, Huo LR and Xuan YX: Simultaneous determination and pharmacokinetics of eight ginsenosides by LC-MS/MS after intravenously infusion of ‘SHENMAI’ injection in dogs. Pak J Pharm Sci. 30:421–427. 2017.PubMed/NCBI

12 

Chang YX, Ding XP, Qi J, Cao J, Kang LY, Zhu DN, Zhang BL and Yu BY: The antioxidant-activity-integrated fingerprint: An advantageous tool for the evaluation of quality of herbal medicines. J Chromatogr A. 1208:76–82. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Zhang JL, Cui M, He Y, Yu HL and Guo DA: Chemical fingerprint and metabolic fingerprint analysis of Danshen injection by HPLC-UV and HPLC-MS methods. J Pharm Biomed Anal. 36:1029–1035. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Gao Y, Chu S, Shao Q, Zhang M, Xia C, Wang Y, Li Y, Luo Y, Huang H and Chen N: Antioxidant activities of ginsenoside Rg1 against cisplatin-induced hepatic injury through Nrf2 signaling pathway in mice. Free Radic Res. 51:1–13. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Yuan SM: Potential cardioprotective effects of Ginseng preparations. Pak J Pharm Sci. 28:963–968. 2015.PubMed/NCBI

16 

Ye LF, Zheng YR and Wang LH: Effects of Shenmai injection and its bioactive components following ischemia/reperfusion in cardiomyocytes. Exp Ther Med. 10:1348–1354. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Han JY, Horie Y, Miura S, Akiba Y, Guo J, Li D, Fan JY, Liu YY, Hu BH, An LH, et al: Compound Danshen injection improves endotoxin-inducedmicrocirculatory disturbance in rat mesentery. World J Gastroenterol. 13:3581–3591. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Geng QX, Zhu XL and Zhang XH: Effect of combined therapy of Shenmai and compound Danshen injection on myocardial reperfusion injury after percutaneous coronary intervention in patients with acute myocardial infarction. Chin J Integr Med. 24:496–499. 2004.

19 

Li YH, Li YY, Fan GW, Zhou K, Duan ZZ, Yu JH and Gao XM: Cardioprotective effects of YiqiYangyinHuoxue injection against ischemia-reperfusion injury in isolated hearts of rats. Chinese Traditional and Herbal Drugs. 47:281–289. 2016.

20 

Ren J, Poon BY, Tang Y, Funk GD and Greer JJ: Ampakines alleviate respiratory depression in rats. Am J Respir Crit Care Med. 174:1384–1391. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Pagliardini S, Ren J and Greer JJ: Ontogeny of the pre-botzinger complex in perinatal rats. J Neurosci. 23:9575–9584. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Wang KK, Ding RR, Ha YP, Jia YN, Liao XM, Wang SS, Li RJ, Shen ZH, Xiong H, Guo JL and Jie W: Hypoxia-stressed cardiomyocytes promote early cardiac differentiation of cardiac stem cells through HIF-1α/Jagged1/Notch1 signaling. Acta Pharm Sin B. 8:795–804. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Duan ZZ, Li YH, Li YY, Fan GW, Chang YX, Yu B and Gao XM: Danhong injection protects cardiomyocytes against hypoxia/reoxygenation- and H2O2-induced injury by inhibiting mitochondrial permeability transition pore opening. J Ethnopharmacol. 175:617–625. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Gao SX: Study on the therapeutic effect of Shenmai injection combined with compound Danshen injection on acute myocardial infarction. Chin J Med Guide. 19:728–730. 2017.

25 

Baracca A, Sgarbi G, Solaini G and Lenaz G: Rhodamine 123 as a probe of mitochondrial membrane potential: Evaluation of proton flux through F(0) during ATP synthesis. Biochim Biophys Acta. 1606:137–146. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Fukumori R, Takarada T, Kambe Y, Nakazato R, Fujikawa K and Yoneda Y: Possible involvement of mitochondrial uncoupling protein-2 in cytotoxicity mediated by acquired N-methyl-D-aspartate receptor channels. Neurochem Int. 61:498–505. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Carrasco-Pozo C, Pastene E, Vergara C, Zapata M, Sandoval C and Gotteland M: Stimulation of cytosolic and mitochondrial calcium mobilization by indomethacin in Caco-2 cells: Modulation by the polyphenols quercetin, resveratrol and rutin. Biochim Biophys Acta. 1820:2052–2061. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Petronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P and Di Lisa F: Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence. Biophys J. 76:725–734. 1999. View Article : Google Scholar : PubMed/NCBI

29 

Sharov VG, Todor A, Khanal S, Imai M and Sabbah HN: Cyclosporine A attenuates mitochondrial permeability transitionand improves mitochondrial respiratory function in cardiomyocytes isolated from dogs with heart failure. J Mol Cell Cardiol. 42:150–158. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Turer AT and Hill JA: Pathogenesis of myocardial ischemia-reperfusion injury and rationale for therapy. Am J Cardiol. 106:360–368. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Yellon DM and Hausenloy DJ: Myocardial reperfusion injury. N Engl J Med. 357:1121–1135. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Wu SD, Xia F, Lin XM, Duan KL, Wang F, Lu QL, Cao H, Qian YH and Shi M: Ginsenoside-Rd promotes neurite outgrowth of PC12 cells through MAPK/ERK- and PI3K/AKT-dependent pathways. Int J Mol Sci. 17:E1772016. View Article : Google Scholar : PubMed/NCBI

33 

Zhou Q, Sun Y, Tan W, Liu X, Qian Y, Ma X, Wang T, Wang X and Gao X: Effect of Shenmai injection on preventing the development of nitroglycerin-induced tolerance in rats. PLoS One. 12:e01767772017. View Article : Google Scholar : PubMed/NCBI

34 

Li YH, Li YY, Fan GW, Yu JH, Duan ZZ, Wang LY and Yu B: Cardioprotection of Ginsenoside Rb1 against ischemia/reperfusion injury is associated with mitochondrial permeability transition pore opening inhibition. Chin J Integr Med. 2016.(Epub ahead of print). View Article : Google Scholar

35 

Yang B, Yan P, Gong H, Zuo L, Shi Y, Guo J, Guo R, Xie J and Li B: TWEAK protects cardiomyocyte against apoptosis in a PI3K/AKT pathway dependent manner. Am J Transl Res. 8:3848–3860. 2016.PubMed/NCBI

36 

Cheng XY, Gu XY, Gao Q, Zong QF, Li XH and Zhang Y: Effects of dexmedetomidine postconditioning on myocardial ischemia and the role of the PI3K/Akt-dependent signaling pathway in reperfusion injury. Mol Med Rep. 14:797–803. 2016. View Article : Google Scholar : PubMed/NCBI

37 

Manning BD and Cantley LC: AKT/PKB signaling: Navigating downstream. Cell. 129:1261–1274. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Crompton M: The mitochondrial permeability transition pore and its role in cell death. Biochem J. 341:233–249. 1999. View Article : Google Scholar : PubMed/NCBI

39 

Li JZ, Yu SY, Wu JH, Shao QR and Dong XM: Paeoniflorin protects myocardial cell from doxorubicin-induced apoptosis through inhibition of NADPH oxidase. Can J Physiol Pharmacol. 90:1569–1575. 2012. View Article : Google Scholar : PubMed/NCBI

40 

Li L, Zhou YF, Li YL, Wang LL, Arai H and Xu Y: In vitro and in vivo antioxidative and hepatoprotective activity of aqueous extract of Cortex Dictamni. World J Gastroenterol. 23:2819–3010. 2017.PubMed/NCBI

41 

Feng Y, Madungwe NB, da Cruz Junho CV and Bopassa JC: Activation of G protein-coupled estrogen receptor 1 at the onset of reperfusion protects the myocardium against ischemia/reperfusion injury by reducing mitochondrial dysfunction and mitophagy. Br J Pharmacol. 174:4329–4344. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li L, Sha Z, Wang Y, Yang D, Li J, Duan Z, Wang H and Li Y: Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening. Exp Ther Med 17: 4643-4652, 2019.
APA
Li, L., Sha, Z., Wang, Y., Yang, D., Li, J., Duan, Z. ... Li, Y. (2019). Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening. Experimental and Therapeutic Medicine, 17, 4643-4652. https://doi.org/10.3892/etm.2019.7462
MLA
Li, L., Sha, Z., Wang, Y., Yang, D., Li, J., Duan, Z., Wang, H., Li, Y."Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening". Experimental and Therapeutic Medicine 17.6 (2019): 4643-4652.
Chicago
Li, L., Sha, Z., Wang, Y., Yang, D., Li, J., Duan, Z., Wang, H., Li, Y."Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4643-4652. https://doi.org/10.3892/etm.2019.7462
Copy and paste a formatted citation
x
Spandidos Publications style
Li L, Sha Z, Wang Y, Yang D, Li J, Duan Z, Wang H and Li Y: Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening. Exp Ther Med 17: 4643-4652, 2019.
APA
Li, L., Sha, Z., Wang, Y., Yang, D., Li, J., Duan, Z. ... Li, Y. (2019). Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening. Experimental and Therapeutic Medicine, 17, 4643-4652. https://doi.org/10.3892/etm.2019.7462
MLA
Li, L., Sha, Z., Wang, Y., Yang, D., Li, J., Duan, Z., Wang, H., Li, Y."Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening". Experimental and Therapeutic Medicine 17.6 (2019): 4643-4652.
Chicago
Li, L., Sha, Z., Wang, Y., Yang, D., Li, J., Duan, Z., Wang, H., Li, Y."Pre‑treatment with a combination of Shenmai and Danshen injection protects cardiomyocytes against hypoxia/reoxygenation‑ and H2O2‑induced injury by inhibiting mitochondrial permeability transition pore opening". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4643-4652. https://doi.org/10.3892/etm.2019.7462
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