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Article

Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis

  • Authors:
    • Yugang Yin
    • Lei Lv
    • Wenni Wang
  • View Affiliations / Copyright

    Affiliations: Department of Geriatric Cardiology, Jinling Hospital Affiliated to Nanjing University, Nanjing, Jiangsu 210093, P.R. China
  • Pages: 4657-4662
    |
    Published online on: April 3, 2019
       https://doi.org/10.3892/etm.2019.7464
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Abstract

The aim of the present study was to investigate the expression of microRNA (miRNA/miR)‑214 in the sera of elderly patients with acute myocardial infarction (AMI) and the mechanism of how its expression affects cardiomyocyte apoptosis in these patients. The expression levels of miRNA‑214 in elderly patients with AMI, unstable angina (UA) and healthy elderly subjects were detected by reverse transcription‑quantitative polymerase chain reaction, and the correlation between the relative expressions of sera miRNA‑214 and myocardial enzymes in elderly patients with AMI was examined by Pearson's analysis. Human cardiomyocyte (HCM) cell lines with a high expression miRNA‑214 were established. The apoptotic rates of the different groups of cells were detected by flow cytometry and TUNEL assay. The expression of miRNA‑214 target genes in the different groups of cells was detected by western blot assay. The relative expression levels of sera miR‑214 in elderly AMI patients, UA patients and healthy subjects (as determined by physical examination) were 15.79±4.66, 4.60±2.51 and 2.07±0.99, respectively. The differences between each group were statistically significant (P<0.05). The relative expression of sera miRNA‑214 in elderly AMI patients was positively correlated with sera aspartate aminotransferase (r=0.361, P=0.0174), lactate dehydrogenase (r=0.425, P=0.0045), creatine kinase‑MB (r=0.835, P<0.001) and cardiac troponin (r=0.770, P<0.001). When compared with normal HCMs, the expressions of p53‑upregulated modulator of apoptosis (PUMA), phosphatase and tensin homolog (PTEN), B‑cell lymphoma‑2‑associated X protein (Bax) and caspase 7 proteins was decreased in HCMs overexpressing miRNA‑214 following H2O2 induction, and the rate of apoptosis decreased by 63.64, 21.95, 46.67 and 50.05%, respectively. miRNA‑214 was highly expressed in the sera of elderly patients with AMI, which may inhibit myocardial cell apoptosis by inhibiting the expression of miR‑214 target genes including PUMA, PTEN, Bax and caspase 7.
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1 

Nichols M, Townsend N, Scarborough P and Rayner M: Cardiovascular disease in Europe 2014: Epidemiological update. Eur Heart J. 35:2950–2959. 2015. View Article : Google Scholar

2 

Yeh RW, Sidney S, Chandra M, Sorel M, Selby JV and Go AS: Population trends in the incidence and outcomes of acute myocardial infarction. N Engl J Med. 362:2155–2165. 2010. View Article : Google Scholar : PubMed/NCBI

3 

White HD and Chew DP: Acute myocardial infarction. Lancet. 372:570–584. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Jiang X, Guo CX, Zeng XJ, Li HH, Chen BX and Du FH: A soluble receptor for advanced glycation end-products inhibits myocardial apoptosis induced by ischemia/reperfusion via the JAK2/STAT3 pathway. Apoptosis. 20:1033–1047. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Yang T, Gu H, Chen X, Fu S, Wang C, Xu H, Feng Q and Ni Y: Cardiac hypertrophy and dysfunction induced by overexpression of miR-214 in vivo. J Surg Res. 192:3172014. View Article : Google Scholar : PubMed/NCBI

6 

Yang T, Zhang GF, Chen XF, Gu HH, Fu SZ, Xu HF, Feng Q and Ni YM: MicroRNA-214 provokes cardiac hypertrophy via repression of EZH2. Biochem Biophys Res Commun. 436:578–584. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Chen Q, Huang J, Wang H, Zhao J, Chen G, Qi J, Lin X, Chen L and Lin Q: Effects of chronic intermittent hypoxia on regulation of miRNA-214 in myocardial apoptosis in rats. Zhonghua Yi Xue Za Zhi. 95:1214–1217. 2015.(In Chinese). PubMed/NCBI

8 

Mendis S, Thygesen K, Kuulasmaa K, Giampaoli S, Mähönen M, Ngu Blackett K and Lisheng L; Writing group on behalf of the participating experts of the WHO consultation for revision of WHO definition of myocardial infarction, : World Health Organization definition of myocardial infarction: 2008–09 revision. Int J Epidemiol. 40:139–146. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Chinese Society of Cardiology of Chinese Medical Association and Editorial Board of Chinese Journal of Cardiology: Guideline for diagnosis and treatment of patients with unstable angina and non-ST-segment elevation myocardial infarction. Zhonghua Xin Xue Guan Bing Za Zhi. 35:295–304. 2007.(In Chinese). PubMed/NCBI

10 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Libby P: Mechanisms of acute coronary syndromes and their implications for therapy. N Engl J Med. 368:20042013. View Article : Google Scholar : PubMed/NCBI

12 

Cao W, Guo Q, Zhang T, Zhong D and Yu Q: Prognostic value of microRNAs in acute myocardial infarction: A systematic review and meta-analysis. Int J Cardiol. 189:79–84. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Rayner K, Dimmeler S, Calin GA, Thum T, Raizman JE and Diamandis EP: Novel biomarkers for acute myocardial infarction: Is microRNA the new kid on the block? Clin Chem. 60:812–817. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Stone NJ, Robinson JG, Lichtenstein AH, Goff DC Jr, Lloyd-Jones DM, Smith SC Jr, Blum C and Schwartz JS; 2013 ACC/AHA Cholesterol Guideline Panel, : Treatment of blood cholesterol to reduce atherosclerotic cardiovascular disease risk in adults: Synopsis of the 2013 american college of cardiology/american heart association cholesterol guideline. Ann Intern Med. 160:339–343. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Lv G, Shao S, Dong H, Bian X, Yang X and Dong S: MicroRNA-214 protects cardiac myocytes against H2O2-induced injury. J Cell Biochem. 115:93–101. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Aurora AB, Mahmoud AI, Luo X, Johnson BA, van Rooij E, Matsuzaki S, Humphries KM, Hill JA, Bassel-Duby R, Sadek HA and Olson EN: MicroRNA-214 protects the mouse heart from ischemic injury by controlling Ca2+ overload and cell death. J Clin Invest. 122:1222–1232. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Choy EY, Siu KL, Kok KH, Lung RW, Tsang CM, To KF, Kwong DL, Tsao SW and Jin DY: An Epstein-Barr virus-encoded microRNA targets PUMA to promote host cell survival. J Exp Med. 205:2551–2560. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Wang F, Li L, Chen Z, Zhu M and Gu Y: MicroRNA-214 acts as a potential oncogene in breast cancer by targeting the PTEN-PI3K/Akt signaling pathway. Int J Mol Med. 37:1421–1428. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Zheng X, Li J, Peng C, Zhao J, Chi J, Meng X, Yun X, Li D, Yu Y, Gao M and Li Y: MicroRNA-24 induces cisplatin resistance by targeting PTEN in human tongue squamous cell carcinoma. Oral Oncol. 51:998–1003. 2015. View Article : Google Scholar : PubMed/NCBI

20 

He J, Tang Y and Tian Y: MicroRNA-214 promotes proliferation and inhibits apoptosis via targeting Bax in nasopharyngeal carcinoma cells. Mol Med Rep. 12:6286–6292. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Liu J, Li Y, Luo M and Yuan Z: MicroRNA-214 inhibits the osteogenic differentiation of human osteoblasts through the direct regulation of baculoviral IAP repeat-containing 7. Exp Cell Res. 351:157–162. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yin Y, Lv L and Wang W: Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis. Exp Ther Med 17: 4657-4662, 2019.
APA
Yin, Y., Lv, L., & Wang, W. (2019). Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis. Experimental and Therapeutic Medicine, 17, 4657-4662. https://doi.org/10.3892/etm.2019.7464
MLA
Yin, Y., Lv, L., Wang, W."Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis". Experimental and Therapeutic Medicine 17.6 (2019): 4657-4662.
Chicago
Yin, Y., Lv, L., Wang, W."Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4657-4662. https://doi.org/10.3892/etm.2019.7464
Copy and paste a formatted citation
x
Spandidos Publications style
Yin Y, Lv L and Wang W: Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis. Exp Ther Med 17: 4657-4662, 2019.
APA
Yin, Y., Lv, L., & Wang, W. (2019). Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis. Experimental and Therapeutic Medicine, 17, 4657-4662. https://doi.org/10.3892/etm.2019.7464
MLA
Yin, Y., Lv, L., Wang, W."Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis". Experimental and Therapeutic Medicine 17.6 (2019): 4657-4662.
Chicago
Yin, Y., Lv, L., Wang, W."Expression of miRNA‑214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4657-4662. https://doi.org/10.3892/etm.2019.7464
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