Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2019 Volume 18 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2019 Volume 18 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair

  • Authors:
    • Shumei Qi
    • Changxi Yang
    • Mingzhen Zhu
    • Hui Chen
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Jinan Maternity and Child Care Hospital, Jinan, Shandong 250001, P.R. China, Department of Stomatology, Zibo Mining Group Central Hospital, Zibo, Shandong 255120, P.R. China, Department of Stomatology, Jinan Stomatological Hospital, Jinan, Shandong 250001, P.R. China, Department of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong 250001, P.R. China
  • Pages: 320-325
    |
    Published online on: May 6, 2019
       https://doi.org/10.3892/etm.2019.7546
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

This study evaluated the effects of oral mucosal transplantation on epidermal growth factor (EGF) and vascular endothelial growth factor C (VEGF‑C) in skin wound repair. Sixty-four rats were randomly separated into group A, B, C and D (16 rats in each group). The right abdomen skin was excised 1, 3, 5 and 7 days after injury, respectively. Oral mucosa of the rat tongue was transplanted to the right abdomen skin. Fourteen days after the healing of the oral mucosa graft, the rat skin full‑thickness model was prepared at the transplant site (the study group) and the contralateral site (the control group). Rats in each group were anesthetized and sacrificed at 1, 3, 5 and 7 days after injury. Expression of EGF and VEGF‑C in skin tissue was detected by RT‑qPCR and ELISA. At 3 days, expression levels of EGF and VEGF‑C mRNA and protein in skin tissue were significantly higher than those at 1 day (P<0.05). At 5 days, expression levels of EGF and VEGF‑C mRNA and protein in skin tissue were significantly higher than those at 3 days (P<0.05). At 7 days, expression levels of EGF mRNA and protein in skin tissue were significantly lower than those at 5 days (P<0.05), while VEGF‑C levels were significantly increased (P<0.05). Expression levels of EGF and VEGF‑C mRNA and protein in the skin tissue of the study group were significantly lower than those in the control group at all days (P<0.05). EGF and VEGF‑C may be involved in scar formation, and play an important role in the process of skin wound repair.
View Figures

Figure 1

Figure 2

View References

1 

Yu J, Wang MY, Tai HC and Cheng NC: Cell sheet composed of adipose-derived stem cells demonstrates enhanced skin wound healing with reduced scar formation. Acta Biomater. 77:191–200. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Asatiani E, Huang WX, Wang A, Rodriguez Ortner E, Cavalli LR, Haddad BR and Gelmann EP: Deletion, methylation, and expression of the NKX3.1 suppressor gene in primary human prostate cancer. Cancer Res. 65:1164–1173. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Tracy LE, Minasian RA and Caterson EJ: Extracellular matrix and dermal fibroblast function in the healing wound. Adv Wound Care (New Rochelle). 5:119–136. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Purba ER, Saita EI and Maruyama IN: Activation of the EGF receptor by ligand binding and oncogenic mutations: The ‘Rotation Model’. Cells. 6:62017. View Article : Google Scholar

5 

Basso FG, Soares DG, Pansani TN, Cardoso LM, Scheffel DL, de Souza Costa CA and Hebling J: Proliferation, migration, and expression of oral-mucosal-healing-related genes by oral fibroblasts receiving low-level laser therapy after inflammatory cytokines challenge. Lasers Surg Med. 48:1006–1014. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Hominick D, Silva A, Khurana N, Liu Y, Dechow PC, Feng JQ, Pytowski B, Rutkowski JM, Alitalo K and Dellinger MT: VEGF-C promotes the development of lymphatics in bone and bone loss. eLife. 7:e343232018. View Article : Google Scholar : PubMed/NCBI

7 

Chilov D, Kukk E, Taira S, Jeltsch M, Kaukonen J, Palotie A, Joukov V and Alitalo K: Genomic organization of human and mouse genes for vascular endothelial growth factor C. J Biol Chem. 272:25176–25183. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Kosten IJ, van de Ven R, Thon M, Gibbs S and de Gruijl TD: Comparative phenotypic and functional analysis of migratory dendritic cell subsets from human oral mucosa and skin. PLoS One. 12:e01803332017. View Article : Google Scholar : PubMed/NCBI

9 

Song IB, Gu H, Han HJ, Lee NY, Cha JY, Son YK and Kwon J: Effects of 7-MEGATM 500 on oxidative stress, inflammation, and skin regeneration in H2O2-treated skin cells. Toxicol Res. 34:103–110. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Sikes RS; Animal Care and Use Committee of the American Society of Mammalogists, : 2016 Guidelines of the American Society of Mammalogists for the use of wild mammals in research and education. J Mammal. 97:663–688. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 [-Delta Delta C(T)] method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Ramos-Lewis W, LaFever KS and Page-McCaw A: A scar-like lesion is apparent in basement membrane after wound repair in vivo. Matrix Biol. 74:101–120. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Occleston NL, Metcalfe AD, Boanas A, Burgoyne NJ, Nield K, O'Kane S and Ferguson MW: Therapeutic improvement of scarring: mechanisms of scarless and scar-forming healing and approaches to the discovery of new treatments. Dermatol Res Pract. 2010:4052622010. View Article : Google Scholar : PubMed/NCBI

14 

DiPietro LA: Angiogenesis and wound repair: When enough is enough. J Leukoc Biol. 100:979–984. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Iglesias-Bartolome R, Uchiyama A, Molinolo AA, Abusleme L, Brooks SR, Callejas-Valera JL, Edwards D, Doci C, Asselin-Labat ML, Onaitis MW, et al: Transcriptional signature primes human oral mucosa for rapid wound healing. Sci Transl Med. 10:eaap87982018. View Article : Google Scholar : PubMed/NCBI

16 

Han J, Jin W, Ho NA, Hong J, Kim YJ, Shin Y, Lee H and Suh JW: Decursin and decursinol angelate improve wound healing by upregulating transcription of genes encoding extracellular matrix remodeling proteins, inflammatory cytokines, and growth factors in human keratinocytes. Biochem Biophys Res Commun. 499:979–984. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Velasquez LS, Sutherland LB, Liu Z, Grinnell F, Kamm KE, Schneider JW, Olson EN and Small EM: Activation of MRTF-A- dependent gene expression with a small molecule promotes myofibroblast differentiation and wound healing. Proc Natl Acad Sci USA. 110:16850–16855. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Kris MG, Natale RB, Herbst RS, Lynch TJ Jr, Prager D, Belani CP, Schiller JH, Kelly K, Spiridonidis H, Sandler A, et al: Efficacy of gefitinib, an inhibitor of the epidermal growth factor receptor tyrosine kinase, in symptomatic patients with non-small cell lung cancer: A randomized trial. JAMA. 290:2149–2158. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Stoddard MA, Herrmann J, Moy L and Moy R: Improvement of atrophic acne scars in skin of color using topical synthetic epidermal growth factor (EGF) serum: A pilot study. J Drugs Dermatol. 16:322–326. 2017.PubMed/NCBI

20 

Shiraha H, Glading A, Gupta K and Wells A: IP-10 inhibits epidermal growth factor-induced motility by decreasing epidermal growth factor receptor-mediated calpain activity. J Cell Biol. 146:243–254. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Schultz G, Rotatori DS and Clark W: EGF and TGF-alpha in wound healing and repair. J Cell Biochem. 45:346–352. 1991. View Article : Google Scholar : PubMed/NCBI

22 

Haase I, Evans R, Pofahl R and Watt FM: Regulation of keratinocyte shape, migration and wound epithelialization by IGF-1- and EGF-dependent signalling pathways. J Cell Sci. 116:3227–3238. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Kim YS, Lew DH, Tark KC, Rah DK and Hong JP: Effect of recombinant human epidermal growth factor against cutaneous scar formation in murine full-thickness wound healing. J Korean Med Sci. 25:589–596. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Leak LV and Jones M: Lymphangiogenesis in vitro: Formation of lymphatic capillary-like channels from confluent monolayers of lymphatic endothelial cells. In Vitro Cell Dev Biol Anim. 30:512–518. 1994. View Article : Google Scholar : PubMed/NCBI

25 

Oh SJ, Jeltsch MM, Birkenhäger R, McCarthy JE, Weich HA, Christ B, Alitalo K and Wilting J: VEGF and VEGF-C: Specific induction of angiogenesis and lymphangiogenesis in the differentiated avian chorioallantoic membrane. Dev Biol. 188:96–109. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Jeltsch M, Kaipainen A, Joukov V, Meng X, Lakso M, Rauvala H, Swartz M, Fukumura D, Jain RK and Alitalo K: Hyperplasia of lymphatic vessels in VEGF-C transgenic mice. Science. 276:1423–1425. 1997. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Qi S, Yang C, Zhu M and Chen H: Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair. Exp Ther Med 18: 320-325, 2019.
APA
Qi, S., Yang, C., Zhu, M., & Chen, H. (2019). Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair. Experimental and Therapeutic Medicine, 18, 320-325. https://doi.org/10.3892/etm.2019.7546
MLA
Qi, S., Yang, C., Zhu, M., Chen, H."Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair". Experimental and Therapeutic Medicine 18.1 (2019): 320-325.
Chicago
Qi, S., Yang, C., Zhu, M., Chen, H."Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair". Experimental and Therapeutic Medicine 18, no. 1 (2019): 320-325. https://doi.org/10.3892/etm.2019.7546
Copy and paste a formatted citation
x
Spandidos Publications style
Qi S, Yang C, Zhu M and Chen H: Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair. Exp Ther Med 18: 320-325, 2019.
APA
Qi, S., Yang, C., Zhu, M., & Chen, H. (2019). Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair. Experimental and Therapeutic Medicine, 18, 320-325. https://doi.org/10.3892/etm.2019.7546
MLA
Qi, S., Yang, C., Zhu, M., Chen, H."Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair". Experimental and Therapeutic Medicine 18.1 (2019): 320-325.
Chicago
Qi, S., Yang, C., Zhu, M., Chen, H."Effect of oral mucosal transplantation on the expression of EGF and VEGF‑C during skin wound repair". Experimental and Therapeutic Medicine 18, no. 1 (2019): 320-325. https://doi.org/10.3892/etm.2019.7546
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team