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Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression

  • Authors:
    • Liping Zhou
    • Zhe Che
    • Xiaowei Zhang
    • Panpan Zhou
    • Xue Li
    • Xuejing Xu
    • Qing Shi
    • Dong Li
    • Xiuli Ju
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The Sixth People's Hospital of Ji'nan, Jinan, Shandong 250200, P.R. China, Health and Family Planning Inspection Agency of Zhangqiu District, Jinan, Shandong 250200, P.R. China, Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 105-118
    |
    Published online on: May 7, 2019
       https://doi.org/10.3892/etm.2019.7549
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Abstract

The present study attempted to evaluate whether neonatal gender affects the hematopoietic potential of cord blood (CB) transplants and, if so, to determine the underlying molecular mechanisms. CD34+ cells from CB were isolated and divided into male and female groups. CD34+CD38‑ cell populations were then compared using fluorescence‑assisted cell sorting (FACS) and a colony formation assay was performed. Next, a Genechip microarray analysis was used to identify differentially expressed genes (DEGs). Finally, the Genechip results were validated by FACS analysis. It was revealed that the male group had higher amplification efficiency. Gene ontology analysis indicated differences in the biological function of the DEGs between the two groups. Kyoto Encyclopedia of Genes and Genomes analysis suggested that the hematopoietic cell lineage signaling pathway was upregulated in the male group along with high expression levels of genes including interleukin (IL) 6 signal transducer (glycoprotein 130), IL‑7 and IL‑7 receptor. It was speculated that this may be partially due to numerous upregulated DEGs being involved in chromosomal segregation and hematopoietic cell lineage signaling pathways in CD34+ cells from the male group.
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1 

Seita J and Weissman IL: Hematopoietic stem cell: Self-renewal versus differentiation. Wiley Interdiscip Rev SystBiol Med. 2:640–653. 2010. View Article : Google Scholar

2 

Möbest D, Mertelsmann R and Henschler R: Serum-free ex vivo expansion of CD34(+) hematopoietic progenitor cells. Biotechnol Bioeng. 60:341–347. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Hao QL, Shah AJ, Thiemann FT, Smogorzewska EM and Crooks GM: A functional comparison of CD34+CD38− cells in cord blood and bone marrow. Blood. 86:3745–3753. 1995.PubMed/NCBI

4 

Bhatia M, Wang JC, Kapp U, Bonnet D and Dick JE: Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice. Proc Natl Acad Sci USA. 94:5320–5325. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Rocha V, Wagner JE Jr, Sobocinski KA, Klein JP, Zhang MJ, Horowitz MM and Gluckman E: Graft-versus-host disease in children who have received a cord-blood or bone marrow transplant from an HLA-identical sibling. Eurocord and international bone marrow transplant registry working committee on alternative donor and stem cell sources. N Engl J Med. 342:1846–1854. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Chou S, Chu P, Hwang W and Lodish H: Expansion of human cord blood hematopoietic stem cells for transplantation. Cell Stem Cell. 7:427–428. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Horwitz ME and Frassoni F: Improving the outcome of umbilical cord blood transplantation through ex vivo expansion or graft manipulation. Cytotherapy. 17:730–738. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Aroviita P, Teramo K, Hiilesmaa V and Kekomäki R: Cord blood hematopoietic progenitor cell concentration and infant sex. Transfusion. 45:613–621. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Bijou F, Ivanovic Z, Fizet D, Dazey B, Boiron JM and Lafarge X: Neonatal sex and weight influence CD34(+) cell concentration in umbilical cord blood but not stromal cell-ederived factor 1–3′ A polymorphism. Cytotherapy. 17:68–72. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Robinson S, Niu T, de Lima M, Ng J, Yang H, McMannis J, Karandish S, Sadeghi T, Fu P, del Angel M, et al: Ex vivo expansion of umbilical cord blood. Cytotherapy. 7:243–250. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Sauvageau G, Iscove NN and Humphries RK: In vitro and in vivo expansion of hematopoietic stem cells. Oncogene. 23:7223–7232. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Ramalho-Santos M, Yoon S, Matsuzaki Y, Mulligan RC and Melton DA: ‘Stem ness’: Transcriptional profiling of embryonic and adult stem cells. Science. 298:597–600. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Fortunel NO, Out HH, Ng HH, Chen J, Mu X, Chevassut T, Li X, Joseph M, Bailey C, Hatzfeld JA, et al: Comment on “‘Stemness’: Transcriptional profiling of embryonic and adult stem cells” and ‘a stem cell molecular signature’. Science. 302:3932003. View Article : Google Scholar : PubMed/NCBI

14 

Ivanova NB, Dimos JT, Schaniel C, Hackney JA, Moore KA and Lemischka IR: A stem cell molecular signature. Science. 298:601–604. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Alves NL, Richard-Le Goff O, Huntington ND, Sousa AP, Ribeiro VS, Bordack A, Vives FL, Peduto L, Chidgey A, Cumano A, et al: Characterization of the thymic IL-7 niche in vivo. Proc Natl Acad Sci USA. 106:1512–1517. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Kimura T, Sakabe H, Tanimukai S, Abe T, Urata Y, Yasukawa K, Okano A, Taga T, Sugiyama H, Kishimoto T and Sonoda Y: Simultaneous activation of signals through gp130, c-kit, and interleukin-3 receptor promotes a trilineage blood cell production in the absence of terminally acting lineage-specific factors. Blood. 90:4767–4778. 1997.PubMed/NCBI

17 

Sonoda Y, Sakabe H, Ohmisono Y, Tanimukai S, Yokota S, Nakagawa S, Clark SC and Abe T: Synergistic actions of stem cell factor and other burst-promoting activities on proliferation of CD34+ highly purified blood progenitors expressing HLA-DR or different levels of c-kit protein. Blood. 84:4099–4106. 1994.PubMed/NCBI

18 

Minamiguchi H, Yahata N, Kimura T, Fujiki H, Harada S, Wang J, Okuda K, Kaneko H, Hodohara K, Banba T, et al: Interleukin-6 receptor expression by human cord blood- or peripheral blood-derived primitive haematopoietic progenitors implies acqusition of different functional properties. Br J Haematol. 110:327–328. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Kimura T, Wang J, Minamiguchi H, Fujiki H, Harada S, Okuda K, Kaneko H, Yokota S, Yasukawa K, Abe T and Sonoda Y: Signal through gp130 activated by soluble interleukin (IL)-6 receptor (R) and IL-6 or IL-6R/IL-6 fusion protein enhances ex vivo expansion of human peripheral blood-derived hematopoietic progenitors. Stem Cells. 18:444–452. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Wolf J, Rose-John S and Garbers C: Interleukin-6 and its receptors: A highly regulated and dynamic system. Cytokine. 70:11–20. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Mihara M, Hashizume M, Yoshida H, Suzuki M and Shiina M: IL-6/IL-6 receptor system and its role in physiological and pathological conditions. Clin Sci (Lond). 122:143–159. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Lapidot T and Petit I: Current understanding of stem cell mobilization: The roles of chemokines, proteolytic enzymes, adhesion molecules, cytokines, and stromal cells. Exp Hematol. 30:973–981. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Nagasawa T, Hirota S, Tachibana K, Takakura N, Nishikawa S, Kitamura Y, Yoshida N, Kikutani H and Kishimoto T: Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature. 382:635–638. 1996. View Article : Google Scholar : PubMed/NCBI

24 

Zou YR, Kottmann AH, Kuroda M, Taniuchi I and Littman DR: Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar development. Nature. 393:595–599. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Wilson A and Trumpp A: Bone-marrow haematopoietic-stem-cell niches. Nat Rev Immunol. 6:93–106. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Lane TA: Umbilical cord blood grafts for hematopoietic transplantation in adults: A cup half empty or half full? Transfusion. 45:1027–1034. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou L, Che Z, Zhang X, Zhou P, Li X, Xu X, Shi Q, Li D and Ju X: Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression. Exp Ther Med 18: 105-118, 2019.
APA
Zhou, L., Che, Z., Zhang, X., Zhou, P., Li, X., Xu, X. ... Ju, X. (2019). Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression. Experimental and Therapeutic Medicine, 18, 105-118. https://doi.org/10.3892/etm.2019.7549
MLA
Zhou, L., Che, Z., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression". Experimental and Therapeutic Medicine 18.1 (2019): 105-118.
Chicago
Zhou, L., Che, Z., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression". Experimental and Therapeutic Medicine 18, no. 1 (2019): 105-118. https://doi.org/10.3892/etm.2019.7549
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou L, Che Z, Zhang X, Zhou P, Li X, Xu X, Shi Q, Li D and Ju X: Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression. Exp Ther Med 18: 105-118, 2019.
APA
Zhou, L., Che, Z., Zhang, X., Zhou, P., Li, X., Xu, X. ... Ju, X. (2019). Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression. Experimental and Therapeutic Medicine, 18, 105-118. https://doi.org/10.3892/etm.2019.7549
MLA
Zhou, L., Che, Z., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression". Experimental and Therapeutic Medicine 18.1 (2019): 105-118.
Chicago
Zhou, L., Che, Z., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Influence of neonatal gender on cord blood CD34+ cell amplification and gene expression". Experimental and Therapeutic Medicine 18, no. 1 (2019): 105-118. https://doi.org/10.3892/etm.2019.7549
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