Effects of etanercept, a tumor necrosis factor receptor fusion protein, on primary cell cultures prepared from intact human intervertebral disc tissue

Caliskan,Tezcan; Yasar Sirin,Duygu; Karaarslan,Numan; Yilmaz,Ibrahim; Ozbek,Hanefi; Akyuva,Yener; Kaplan,Necati; Kaya,Yasin  Emre; Simsek,Abdullah  Talha; Yildirim Guzelant,Aliye; Ates,Ozkan

doi:10.3892/etm.2019.7559

Effects of etanercept, a tumor necrosis factor receptor fusion protein, on primary cell cultures prepared from intact human intervertebral disc tissue

Authors:
- Tezcan Caliskan
- Duygu Yasar Sirin
- Numan Karaarslan
- Ibrahim Yilmaz
- Hanefi Ozbek
- Yener Akyuva
- Necati Kaplan
- Yasin Emre Kaya
- Abdullah Talha Simsek
- Aliye Yildirim Guzelant
- Ozkan Ates
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Affiliations: Department of Neurosurgery, Namik Kemal University School of Medicine, Tekirdag 59100, Turkey, Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag 59100, Turkey, Department of Medical Pharmacology, Istanbul Medipol University School of Medicine, Istanbul 34810, Turkey, Department of Neurosurgery, Gaziosmanpasa Taksim Training and Research Hospital, Istanbul 34433, Turkey, Department of Neurosurgery, Corlu Reyap Hospital, Istanbul Rumeli University, Tekirdag 59680, Turkey, Department of Orthopedic and Traumatology, Abant Izzet Basal University School of Medicine, Bolu 14000, Turkey, Department of Physical Medicine and Rehabilitation, Corlu Reyap Hospital, Istanbul Rumeli University, Tekirdag 59680, Turkey, Department of Neurosurgery, Esencan Hospital, Istanbul Esenyurt University, Istanbul 34570, Turkey
Published online on: May 8, 2019 https://doi.org/10.3892/etm.2019.7559
Pages: 69-76
Copyright: © Caliskan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effects of etanercept (ETA), a tumor necrosis factor (TNF) inhibitor, on human cell cultures prepared from intact intervertebral disc tissue. ETA is used as a treatment for cases of rheumatoid arthritis, psoriatic arthritis, axial spondyloarthritis and ankylosing spondylitis accompanied by moderate or severe joint pain. ETA was applied to primary cell cultures [annulus fibrosus and nucleus pulposus (NP) from intact intervertebral disc tissue]. Cell cultures without ETA treatment served as the control group. Morphological and quantitative molecular analyses of the two groups were performed. The number of viable cells and cell proliferation decreased in the ETA‑treated cultures as compared with those in the control group. Furthermore, in the treatment group, the chondroadherin gene, an NP‑specific marker, was not expressed after 24 h. By contrast, the cartilage oligo matrix protein was expressed 24, 48 and 72 h post‑ETA treatment, while its expression was significantly lower than that in the control group. In addition, the expression of interleukin‑1β, as well as matrix metallopeptidase‑7 and ‑19, was markedly decreased. Overall, the cell proliferation and gene expression in the ETA‑treated cells were significantly different from those in the control group (P<0.05). These results suggest that the treatment duration and dosage of TNF inhibitors, which are used to suppress active inflammation, should be considered in the clinical setting. These biological agents may delay the healing of intervertebral disc tissue damage by slowing cell proliferation and altering gene expression via anabolic and catabolic pathways.

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