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Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue

  • Authors:
    • Feride Sinem Akgun
    • Duygu Yasar Sirin
    • Ibrahim Yilmaz
    • Numan Karaarslan
    • Hanefi Ozbek
    • Abdullah Talha Simsek
    • Yasin Emre Kaya
    • Necati Kaplan
    • Yener Akyuva
    • Tezcan Caliskan
    • Ozkan Ates
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, School of Medicine, Istanbul Maltepe University, Istanbul 34843, Turkey, Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag 59100, Turkey , Department of Medical Pharmacology, School of Medicine, Istanbul Medipol University, Istanbul 34810, Turkey, Department of Neurosurgery, School of Medicine, Namik Kemal University, Tekirdag 59100, Turkey, Department of Orthopedics and Traumatology, School of Medicine, Abant Izzet Baysal University, Bolu 14000, Turkey, Department of Neurosurgery, Corlu Reyap Hospital, Istanbul Rumeli University, Tekirdag 59680, Turkey, Department of Neurosurgery, Gaziosmanpasa Taksim Training and Research Hospital, Istanbul 34433, Turkey, Department of Neurosurgery, Istanbul Koc University Hospital, Istanbul Koc University, Istanbul 34010, Turkey
    Copyright: © Akgun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 216-224
    |
    Published online on: May 13, 2019
       https://doi.org/10.3892/etm.2019.7576
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Abstract

The present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti‑pyretic and anti‑spasmodic used in emergency departments, on nucleus pulposus and annulus fibrosus cells in vitro. After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered to the cultures in the experimental groups except for the control group. The data obtained were statistically evaluated. The proliferation was identified to be suppressed via MTT analysis. The gene expression profile of the intervertebral disc cells in the dipyrone‑treated groups was significantly changed. The expression of chondroadherin, cartilage oligo matrix protein, interleukin‑1β and metalloproteinase (MMP)‑19 genes were decreased, but MMP‑13 and MMP‑7 genes expressions were increased, as determined via reverse transcription‑quantitative PCR. AO/PI staining revealed that no apoptotic or other type of cell death was detectable after administration of dipyrone does not mean that the drug is innocuous. The occurrence of cellular senescence and/or the halt of cell proliferation may also be important mechanisms underlying the adverse inhibitory effects of dipyrone. Therefore, prior to administering dipyrone in clinical practice, all possible adverse effects of this drug should be considered.
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Copy and paste a formatted citation
Spandidos Publications style
Akgun FS, Sirin DY, Yilmaz I, Karaarslan N, Ozbek H, Simsek AT, Kaya YE, Kaplan N, Akyuva Y, Caliskan T, Caliskan T, et al: Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue. Exp Ther Med 18: 216-224, 2019.
APA
Akgun, F.S., Sirin, D.Y., Yilmaz, I., Karaarslan, N., Ozbek, H., Simsek, A.T. ... Ates, O. (2019). Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue. Experimental and Therapeutic Medicine, 18, 216-224. https://doi.org/10.3892/etm.2019.7576
MLA
Akgun, F. S., Sirin, D. Y., Yilmaz, I., Karaarslan, N., Ozbek, H., Simsek, A. T., Kaya, Y. E., Kaplan, N., Akyuva, Y., Caliskan, T., Ates, O."Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue". Experimental and Therapeutic Medicine 18.1 (2019): 216-224.
Chicago
Akgun, F. S., Sirin, D. Y., Yilmaz, I., Karaarslan, N., Ozbek, H., Simsek, A. T., Kaya, Y. E., Kaplan, N., Akyuva, Y., Caliskan, T., Ates, O."Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue". Experimental and Therapeutic Medicine 18, no. 1 (2019): 216-224. https://doi.org/10.3892/etm.2019.7576
Copy and paste a formatted citation
x
Spandidos Publications style
Akgun FS, Sirin DY, Yilmaz I, Karaarslan N, Ozbek H, Simsek AT, Kaya YE, Kaplan N, Akyuva Y, Caliskan T, Caliskan T, et al: Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue. Exp Ther Med 18: 216-224, 2019.
APA
Akgun, F.S., Sirin, D.Y., Yilmaz, I., Karaarslan, N., Ozbek, H., Simsek, A.T. ... Ates, O. (2019). Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue. Experimental and Therapeutic Medicine, 18, 216-224. https://doi.org/10.3892/etm.2019.7576
MLA
Akgun, F. S., Sirin, D. Y., Yilmaz, I., Karaarslan, N., Ozbek, H., Simsek, A. T., Kaya, Y. E., Kaplan, N., Akyuva, Y., Caliskan, T., Ates, O."Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue". Experimental and Therapeutic Medicine 18.1 (2019): 216-224.
Chicago
Akgun, F. S., Sirin, D. Y., Yilmaz, I., Karaarslan, N., Ozbek, H., Simsek, A. T., Kaya, Y. E., Kaplan, N., Akyuva, Y., Caliskan, T., Ates, O."Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue". Experimental and Therapeutic Medicine 18, no. 1 (2019): 216-224. https://doi.org/10.3892/etm.2019.7576
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