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Expression levels and significance of miR-184 and miR-126 in burned rats

  • Authors:
    • Sida Xu
    • Youfen Fan
    • Tianbin Wu
    • Pei Xu
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Burn Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 483-488
    |
    Published online on: May 13, 2019
       https://doi.org/10.3892/etm.2019.7578
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Abstract

Expression levels and significance of miR-184 and miR-126 in burned rats were investigated. A total of 30 healthy rats were selected to construct a burn rat model, and another 10 healthy rats as the control group. The modeled rats were divided into groups I, II and III according to burn area, 10 for each group. The reverse transcription‑quantitative polymerase chain reaction (RT-q.PCR) was used to detect the expression of miR-184 and miR-126 in the serum of three groups of burned rats, and ELISA was employed to detect the expression levels in peripheral blood and the correlation. There was no difference in the expression levels of miR-184 and miR-126 among the four groups of rats before modeling (P>0.05). Those of miR-184 and miR-126 at each time point were lower than those at the previous one in groups I, II and III (P<0.05). There was no significant change in the expression levels of miR-184 and miR-126 in the control group (P>0.05). At the same time point, those of miR-184 and miR-126 decreased with the increase of burn degree, and the difference was statistically significant between every two groups (P<0.05). The results of Pearson's correlation analysis revealed that the expression level of miR-184 was positively correlated with that of miR-126 (r=0.832, P=0.002). miR-184 and miR-126 were positively correlated with burn degree (r=0.901, P=0.001, r=0.775, P=0.001) and time after burn (r=0.732, P=0.004, r=0.753, P=0.002). The expression levels of miR-184 and miR-126 decrease in burned rats. The changes of their levels may be used as a reference standard for clinical efficacy evaluation to evaluate burn degree, preventing burn wounds from deepening.
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Copy and paste a formatted citation
Spandidos Publications style
Xu S, Fan Y, Wu T and Xu P: Expression levels and significance of miR-184 and miR-126 in burned rats. Exp Ther Med 18: 483-488, 2019.
APA
Xu, S., Fan, Y., Wu, T., & Xu, P. (2019). Expression levels and significance of miR-184 and miR-126 in burned rats. Experimental and Therapeutic Medicine, 18, 483-488. https://doi.org/10.3892/etm.2019.7578
MLA
Xu, S., Fan, Y., Wu, T., Xu, P."Expression levels and significance of miR-184 and miR-126 in burned rats". Experimental and Therapeutic Medicine 18.1 (2019): 483-488.
Chicago
Xu, S., Fan, Y., Wu, T., Xu, P."Expression levels and significance of miR-184 and miR-126 in burned rats". Experimental and Therapeutic Medicine 18, no. 1 (2019): 483-488. https://doi.org/10.3892/etm.2019.7578
Copy and paste a formatted citation
x
Spandidos Publications style
Xu S, Fan Y, Wu T and Xu P: Expression levels and significance of miR-184 and miR-126 in burned rats. Exp Ther Med 18: 483-488, 2019.
APA
Xu, S., Fan, Y., Wu, T., & Xu, P. (2019). Expression levels and significance of miR-184 and miR-126 in burned rats. Experimental and Therapeutic Medicine, 18, 483-488. https://doi.org/10.3892/etm.2019.7578
MLA
Xu, S., Fan, Y., Wu, T., Xu, P."Expression levels and significance of miR-184 and miR-126 in burned rats". Experimental and Therapeutic Medicine 18.1 (2019): 483-488.
Chicago
Xu, S., Fan, Y., Wu, T., Xu, P."Expression levels and significance of miR-184 and miR-126 in burned rats". Experimental and Therapeutic Medicine 18, no. 1 (2019): 483-488. https://doi.org/10.3892/etm.2019.7578
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