miR‑25‑3p promotes glioma cell proliferation and migration by targeting FBXW7 and DKK3

Peng,Gang; Yang,Chenxing; Liu,Yi; Shen,Chenfu

doi:10.3892/etm.2019.7583

miR‑25‑3p promotes glioma cell proliferation and migration by targeting FBXW7 and DKK3

Authors:
- Gang Peng
- Chenxing Yang
- Yi Liu
- Chenfu Shen
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Affiliations: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
Published online on: May 15, 2019 https://doi.org/10.3892/etm.2019.7583
Pages: 769-778

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Abstract

MicroRNAs (miRs) serve important roles in glioma. However, the underlying molecular mechanism of miR‑25 in glioma progression remains largely unknown; therefore, it was investigated in the present study. RT‑qPCR analysis revealed that miR‑25 expression levels were markedly increased in human glioma tissue and glioma cell lines compared with normal brain tissues and normal human astrocytes, respectively. miR‑25 upregulation exhibited an association with glioma progression, and the knockdown of miR‑25 significantly inhibited glioma cell proliferation and migration. F‑box and WD repeat domain containing 7 (FBXW7) and dickkopf WNT signaling pathway inhibitor 3 (DKK3) were identified as target genes of miR‑25. FBXW7 and DKK3 expression levels were significantly downregulated in glioma tissue samples compared with normal brain tissue, and their expression levels were negatively regulated by miR‑25 expression in glioma cells. Furthermore, inhibition of FBXW7 and DKK3 expression suppressed the miR‑25‑induced effects on glioma cell proliferation and migration. The findings of the present study suggest that miR‑25 may promote glioma cell proliferation and migration by inhibiting the expression of FBXW7 and DKK3. Therefore, miR‑25 may serve as a promising molecular target for the treatment of glioma.

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