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Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1

  • Authors:
    • Ruoxi Yin
    • Xiaoya Chen
  • View Affiliations / Copyright

    Affiliations: Postgraduate School, Xuzhou Medical University, Xuzhou Eye Hospital, Xuzhou, Jiangsu 221000, P.R. China, Department of Ophthalmology, Xuzhou No. 1 People's Hospital, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou Eye Hospital, Xuzhou, Jiangsu 221000, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 647-653
    |
    Published online on: May 15, 2019
       https://doi.org/10.3892/etm.2019.7584
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Abstract

Role of microRNA (miR)‑144‑3p in regulating the function of human trabecular meshwork cells (HTMCs) and fibronectin‑1 (FN‑1) was investigated. HTM cell lines were divided into five groups, of which four groups established oxidative stress HTMC models and one served as a control group. The four model groups were transfected with miR‑144‑3p independent sequence, inhibitory sequence, over‑expression sequence, and the blank group received no transfection. In addition, 40 primary open angle glaucoma patients treated in Xuzhou No. 1 People's Hospital were included in the observation group, and 40 healthy individuals were enrolled as a normal group. RT‑qPCR was used for the detection of miR‑144‑3p expression in serum and cells of patients and healthy people in each group, western blot analysis for FN‑1 expression in cells, CCK‑8 kit for cell proliferation, and Transwell for cell invasion. The expression of serum miR‑144‑3p in the observation group was significantly lower than that in the normal group (P<0.05). The cell optical density value in the over‑expression group was significantly higher than that in the other groups (P<0.05), and in the inhibition group was significantly lower than that in the other groups (P<0.05). The number of cell‑penetration in the over‑expression group was significantly higher than that in the other groups (P<0.05). The expression of FN‑1 protein in the over‑expression group was significantly lower than that in the other four groups (P<0.05), and the expression in the inhibition group was significantly higher than that in the other four groups (P<0.05). The over‑expression of miR‑144‑3p promotes proliferation and invasion of HTMCs by inhibiting the expression of FN‑1 in inoxidative stress HTMCs, and is a potential target for glaucoma treatment.
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1 

Zetterberg M: Age-related eye disease and gender. Maturitas. 83:19–26. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Quigley HA and Broman AT: The number of people with glaucoma worldwide in 2010 and 2020. Br J Ophthalmol. 90:262–267. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Kapetanakis VV, Chan MP, Foster PJ, Cook DG, Owen CG and Rudnicka AR: Global variations and time trends in the prevalence of primary open angle glaucoma (POAG): A systematic review and meta-analysis. Br J Ophthalmol. 100:86–93. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Tamm ER, Braunger BM and Fuchshofer R: Intraocular pressure and the mechanisms involved in resistance of the aqueous humor flow in the trabecular meshwork outflow pathways. Prog Mol Biol Transl Sci. 134:301–314. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Braunger BM, Fuchshofer R and Tamm ER: The aqueous humor outflow pathways in glaucoma: A unifying concept of disease mechanisms and causative treatment. Eur J Pharm Biopharm 95 (Pt B). 173–181. 2015. View Article : Google Scholar

6 

Ha M and Kim VN: Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol. 15:509–524. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Lin S and Gregory RI: MicroRNA biogenesis pathways in cancer. Nat Rev Cancer. 15:321–333. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Condorelli G, Latronico MV and Cavarretta E: microRNAs in cardiovascular diseases: Current knowledge and the road ahead. J Am Coll Cardiol. 63:2177–2187. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Zhang QL, Wang W, Li J, Tian SY and Zhang TZ: Decreased miR-187 induces retinal ganglion cell apoptosis through upregulating SMAD7 in glaucoma. Biomed Pharmacother. 75:19–25. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Zhang SY, Lu ZM, Lin YF, Chen LS, Luo XN, Song XH, Chen SH and Wu YL: miR-144-3p, a tumor suppressive microRNA targeting ETS-1 in laryngeal squamous cell carcinoma. Oncotarget. 7:11637–11650. 2016.PubMed/NCBI

11 

Chen B, Luo L, Wei X, Gong D and Jin L: Altered plasma miR-144 as a novel biomarker for coronary artery disease. Ann Clin Lab Sci. 48:440–445. 2018.PubMed/NCBI

12 

Pankov R and Yamada KM: Fibronectin at a glance. J Cell Sci. 115:3861–3863. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Medina-Ortiz WE, Belmares R, Neubauer S, Wordinger RJ and Clark AF: Cellular fibronectin expression in human trabecular meshwork and induction by transforming growth factor-β2. Invest Ophthalmol Vis Sci. 54:6779–6788. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Liang W, Xie Z, Cui W, Guo Y, Xu L, Wu J and Guan H: Comprehensive gene and microRNA expression profiling reveals a role for miRNAs in the oncogenic roles of SphK1 in papillary thyroid cancer. J Cancer Res Clin Oncol. 143:601–611. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Fry LE, Fahy E, Chrysostomou V, Hui F, Tang J, van Wijngaarden P, Petrou S and Crowston JG: The coma in glaucoma: Retinal ganglion cell dysfunction and recovery. Prog Retin Eye Res. 65:77–92. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Vranka JA, Kelley MJ, Acott TS and Keller KE: Extracellular matrix in the trabecular meshwork: Intraocular pressure regulation and dysregulation in glaucoma. Exp Eye Res. 133:112–125. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Li G, Luna C and Gonzalez P: miR-183 inhibits UV-induced DNA damage repair in human trabecular meshwork cells by targeting of KIAA0101. Invest Ophthalmol Vis Sci. 57:2178–2186. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Li R, Jin Y, Li Q, Sun X, Zhu H and Cui H: MiR-93-5p targeting PTEN regulates the NMDA-induced autophagy of retinal ganglion cells via AKT/mTOR pathway in glaucoma. Biomed Pharmacother. 100:1–7. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Rasmussen KD, Simmini S, Abreu-Goodger C, Bartonicek N, Di Giacomo M, Bilbao-Cortes D, Horos R, Von Lindern M, Enright AJ and O'Carroll D: The miR-144/451 locus is required for erythroid homeostasis. J Exp Med. 207:1351–1358. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Zhang X, Wang X, Zhu H, Zhu C, Wang Y, Pu WT, Jegga AG and Fan GC: Synergistic effects of the GATA-4-mediated miR-144/451 cluster in protection against simulated ischemia/reperfusion-induced cardiomyocyte death. J Mol Cell Cardiol. 49:841–850. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Liu Z, Yi J, Ye R, Liu J, Duan Q, Xiao J and Liu F: miR-144 regulates transforming growth factor-β1 iduced hepatic stellate cell activation in human fibrotic liver. Int J Clin Exp Pathol. 8:3994–4000. 2015.PubMed/NCBI

23 

Ruibin W, Zheng X, Chen J, Zhang X, Yang X and Lin Y: Micro RNA-1298 opposes the effects of chronic oxidative stress on human trabecular meshwork cells via targeting on EIF4E3. Biomed Pharmacother. 100:349–357. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Singh P, Carraher C and Schwarzbauer JE: Assembly of fibronectin extracellular matrix. Annu Rev Cell Dev Biol. 26:397–419. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Babizhayev MA and Brodskaya MW: Fibronectin detection in drainage outflow system of human eyes in ageing and progression of open-angle glaucoma. Mech Ageing Dev. 47:145–157. 1989. View Article : Google Scholar : PubMed/NCBI

26 

Filla MS, Dimeo KD, Tong T and Peters DM: Disruption of fibronectin matrix affects type IV collagen, fibrillin and laminin deposition into extracellular matrix of human trabecular meshwork (HTM) cells. Exp Eye Res. 165:7–19. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yin R and Chen X: Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1. Exp Ther Med 18: 647-653, 2019.
APA
Yin, R., & Chen, X. (2019). Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1. Experimental and Therapeutic Medicine, 18, 647-653. https://doi.org/10.3892/etm.2019.7584
MLA
Yin, R., Chen, X."Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1". Experimental and Therapeutic Medicine 18.1 (2019): 647-653.
Chicago
Yin, R., Chen, X."Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1". Experimental and Therapeutic Medicine 18, no. 1 (2019): 647-653. https://doi.org/10.3892/etm.2019.7584
Copy and paste a formatted citation
x
Spandidos Publications style
Yin R and Chen X: Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1. Exp Ther Med 18: 647-653, 2019.
APA
Yin, R., & Chen, X. (2019). Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1. Experimental and Therapeutic Medicine, 18, 647-653. https://doi.org/10.3892/etm.2019.7584
MLA
Yin, R., Chen, X."Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1". Experimental and Therapeutic Medicine 18.1 (2019): 647-653.
Chicago
Yin, R., Chen, X."Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1". Experimental and Therapeutic Medicine 18, no. 1 (2019): 647-653. https://doi.org/10.3892/etm.2019.7584
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