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Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway

  • Authors:
    • Huanhuan Jiang
    • Junyan Duan
    • Kaihong Xu
    • Wenbo Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 459-466
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    Published online on: May 20, 2019
       https://doi.org/10.3892/etm.2019.7594
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Abstract

The aim of the present study was to explore the protective role of resveratrol (RES) in asthma‑induced airway inflammation and remodeling, as well as its underlying mechanism. An asthma rat model was induced by ovalbumin (OVA) treatment. Rats were randomly assigned into sham, asthma, 10 µmol/l RES and 50 µmol/l RES groups. The amount of inflammatory cells in rat bronchoalveolar lavage fluid (BALF) was detected. Pathological lesions in lung tissues were accessed by hematoxylin and eosin (H&E), and Masson's trichrome staining. Levels of inflammatory factors in lung homogenate were detected via ELISA. The blood serum of asthmatic and healthy children was also collected for analysis. Reverse transcription‑quantitative polymerase chain reaction was performed to detect high mobility group box 1 (HMGB1), Τoll‑like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88) and NF‑κB expression in asthmatic and healthy children, as well as rats of the different groups. H&E staining demonstrated that multiple inflammatory cell infiltration into the rat airway epithelium of the asthma group occurred whilst the 50 µmol/l RES group displayed alleviated pathological lesions. Masson staining indicated that there was an increased airway collagen deposition area in the asthma and 10 µmol/l RES groups compared with the 50 µmol/l RES group. The number of inflammatory cells in BALF extracted from rats of the asthma and 10 µmol/l RES groups was higher compared with the 50 µmol/l RES group. Treatment with 50 µmol/l RES significantly decreased the thicknesses of the airway wall and smooth muscle. ELISA results illustrated that interleukin (IL)‑1, IL‑10 and tumor necrosis factor‑α (TNF‑α) levels were elevated, whereas IL‑12 level was reduced in lung tissues of the asthma and 10 µmol/l RES groups whilst the 50 µmol/l RES group demonstrated the opposite trend. HMGB1, TLR4, MyD88 and NF‑κB mRNA levels were remarkably elevated in rats of the asthma and 10 µmol/l RES groups compared with the 50 µmol/l RES group. Serum levels of IL‑1, IL‑10 and TNF‑α were elevated, whereas IL‑12 was reduced in asthmatic children compared with healthy children. The present results demonstrated that a large dose of RES alleviated asthma‑induced airway inflammation and airway remodeling by inhibiting the release of inflammatory cytokines via the HMGB1/TLR4/NF‑κB pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Duan J, Xu K and Zhang W: Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway. Exp Ther Med 18: 459-466, 2019.
APA
Jiang, H., Duan, J., Xu, K., & Zhang, W. (2019). Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway. Experimental and Therapeutic Medicine, 18, 459-466. https://doi.org/10.3892/etm.2019.7594
MLA
Jiang, H., Duan, J., Xu, K., Zhang, W."Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway". Experimental and Therapeutic Medicine 18.1 (2019): 459-466.
Chicago
Jiang, H., Duan, J., Xu, K., Zhang, W."Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway". Experimental and Therapeutic Medicine 18, no. 1 (2019): 459-466. https://doi.org/10.3892/etm.2019.7594
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Duan J, Xu K and Zhang W: Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway. Exp Ther Med 18: 459-466, 2019.
APA
Jiang, H., Duan, J., Xu, K., & Zhang, W. (2019). Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway. Experimental and Therapeutic Medicine, 18, 459-466. https://doi.org/10.3892/etm.2019.7594
MLA
Jiang, H., Duan, J., Xu, K., Zhang, W."Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway". Experimental and Therapeutic Medicine 18.1 (2019): 459-466.
Chicago
Jiang, H., Duan, J., Xu, K., Zhang, W."Resveratrol protects against asthma‑induced airway inflammation and remodeling by inhibiting the HMGB1/TLR4/NF‑κB pathway". Experimental and Therapeutic Medicine 18, no. 1 (2019): 459-466. https://doi.org/10.3892/etm.2019.7594
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