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Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro

  • Authors:
    • Li Li
    • Lei Zhang
    • Xiongqi Zhao
    • Jun Cao
    • Jingfeng Li
    • Guang Chu
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, First People's Hospital of Kunming City, Kunming, Yunnan 650034, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 425-434
    |
    Published online on: May 20, 2019
       https://doi.org/10.3892/etm.2019.7595
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Abstract

The Gli family is known to be required for the activation of hedgehog signalling, which participates in the pathogenesis of liver fibrosis. The aim of the present study was to identify the association between microRNA (miR)‑152 and GLI family zinc finger 3 (Gli3) and their roles in liver fibrosis. In a carbon tetrachloride (CCl4)‑treated rat model, fibrogenesis‑associated indexes, including hydroxyproline content, collagen deposition, and α‑smooth muscle actin (α‑SMA) and albumin expression, were examined in in vivo and in vitro models. The expression of miR‑152 and Gli3 in cells and tissues was determined by reverse transcription quantitative polymerase chain reaction and western blot analysis. The interaction of Gli3 and miR‑152 was evaluated by bioinformatical analysis and a dual‑luciferase reporter assay. The results demonstrated that miR‑152 was significantly downregulated in serum samples from clinical patients, liver tissues from CCl4‑treated rats and activated LX2 cells. Furthermore, at the cellular level, the mRNA and protein expression levels of α‑SMA and albumin were increased and decreased, respectively, in LX2 cells. Nevertheless, following transfection with an miR‑152 mimic, the expression levels of α‑SMA and albumin were reversed, and Gli3 expression was notably decreased in LX2 cells. Additionally, the target interaction between miR‑152 and Gli3 was demonstrated. Finally, an miR‑152 mimic was introduced into the rat model and additionally demonstrated that the changes in α‑SMA, albumin and Gli3 expression levels were similar to the expression pattern in LX2 cells following miR‑152 mimic transfection. These data provided insight into the potential function of miR‑152 as an anti‑fibrotic therapy through the modulation of Gli3.
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Copy and paste a formatted citation
Spandidos Publications style
Li L, Zhang L, Zhao X, Cao J, Li J and Chu G: Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro. Exp Ther Med 18: 425-434, 2019.
APA
Li, L., Zhang, L., Zhao, X., Cao, J., Li, J., & Chu, G. (2019). Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro. Experimental and Therapeutic Medicine, 18, 425-434. https://doi.org/10.3892/etm.2019.7595
MLA
Li, L., Zhang, L., Zhao, X., Cao, J., Li, J., Chu, G."Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro". Experimental and Therapeutic Medicine 18.1 (2019): 425-434.
Chicago
Li, L., Zhang, L., Zhao, X., Cao, J., Li, J., Chu, G."Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro". Experimental and Therapeutic Medicine 18, no. 1 (2019): 425-434. https://doi.org/10.3892/etm.2019.7595
Copy and paste a formatted citation
x
Spandidos Publications style
Li L, Zhang L, Zhao X, Cao J, Li J and Chu G: Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro. Exp Ther Med 18: 425-434, 2019.
APA
Li, L., Zhang, L., Zhao, X., Cao, J., Li, J., & Chu, G. (2019). Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro. Experimental and Therapeutic Medicine, 18, 425-434. https://doi.org/10.3892/etm.2019.7595
MLA
Li, L., Zhang, L., Zhao, X., Cao, J., Li, J., Chu, G."Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro". Experimental and Therapeutic Medicine 18.1 (2019): 425-434.
Chicago
Li, L., Zhang, L., Zhao, X., Cao, J., Li, J., Chu, G."Downregulation of miR‑152 contributes to the progression of liver fibrosis via targeting Gli3 in vivo and in vitro". Experimental and Therapeutic Medicine 18, no. 1 (2019): 425-434. https://doi.org/10.3892/etm.2019.7595
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