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Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135

  • Authors:
    • Ye Zhang
    • Rui Sui
    • Yi Chen
    • Haiyang Liang
    • Ji Shi
    • Haozhe Piao
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 475-482
    |
    Published online on: May 23, 2019
       https://doi.org/10.3892/etm.2019.7600
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Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs that serve pivotal roles in human diseases. Several miRNAs, such as miR‑485‑3p, have been identified as potential biomarkers for predicting overall survival of patients with glioblastoma (GBM). However, the underlying mechanism of miRNAs in promoting GBM progression remains unknown. In the present study, decreased miR‑485‑3p expression was detected in tumor tissues from patients with GBM. Using western blot analysis, reverse transcription‑quantitative PCR and dual luciferase reporter assay, ring finger protein 135 (RNF135) was confirmed as a target gene of miR‑485‑3p in GBM cells. Through silencing of RNF135, miR‑485‑3p inactivated the mitogen‑activated protein kinase/ERK1/2 pathway in GBM cells. Moreover, functional assays demonstrated that miR‑485‑3p inhibited GBM cell proliferation and migration whilst overexpression of RNF135 reversed this effect. Additionally, a negative correlation between miR‑485‑3p and RNF135 mRNA expression was observed in tissues from patients with glioblastoma. In conclusion, the present results demonstrated that miR‑485‑3p functioned as a tumor suppressor which suggested that miR‑485‑3p might have a role in GBM progression.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2018. CA Cancer J Clin. 68:7–30. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Wick W and Platten M: Understanding and treating glioblastoma. Neurol Clin. 36:485–499. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Buckner JC: Factors influencing survival in high-grade gliomas. Semin Oncol. 30:10–14. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Das S and Marsden PA: Angiogenesis in glioblastoma. N Engl J Med. 369:1561–1563. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Fine HA: The basis for current treatment recommendations for malignant gliomas. J Neurooncol. 20:111–120. 1994. View Article : Google Scholar : PubMed/NCBI

6 

Wick W, Osswald M, Wick A and Winkler F: Treatment of glioblastoma in adults. Ther Adv Neurol Disord. 11:17562864187904522018. View Article : Google Scholar : PubMed/NCBI

7 

Nguyen HS, Shabani S, Awad AJ, Kaushal M and Doan N: Molecular markers of therapy-resistant glioblastoma and potential strategy to combat resistance. Int J Mol Sci. 19(pii): E17652018. View Article : Google Scholar : PubMed/NCBI

8 

Osswald M, Jung E, Sahm F, Solecki G, Venkataramani V, Blaes J, Weil S, Horstmann H, Wiestler B, Syed M, et al: Brain tumour cells interconnect to a functional and resistant network. Nature. 528:93–98. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Lefranc F, Le Rhun E, Kiss R and Weller M: Glioblastoma quo vadis: Will migration and invasiveness reemerge as therapeutic targets? Cancer Treat Rev. 68:145–154. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Onken J, Torka R, Korsing S, Radke J, Krementeskaia I, Nieminen M, Bai X, Ullrich A, Heppner F and Vajkoczy P: Inhibiting receptor tyrosine kinase AXL with small molecule inhibitor BMS-777607 reduces glioblastoma growth, migration and invasion in vitro and in vivo. Oncotarget. 9:9876–9889. 2016.

11 

Lee RC, Feinbaum RL and Ambros V: The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 75:843–854. 1993. View Article : Google Scholar : PubMed/NCBI

12 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Sumazin P, Yang X, Chiu HS, Chung WJ, Iyer A, Llobet-Navas D, Rajbhandari P, Bansal M, Guarnieri P, Silva J and Califano A: An extensive microRNA-mediated network of RNA-RNA interactions regulates established oncogenic pathways in glioblastoma. Cell. 147:370–381. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Yue S, Wang L, Zhang H, Min Y, Lou Y, Sun H, Jiang Y, Zhang W, Liang A, Guo Y, et al: miR-139-5p suppresses cancer cell migration and invasion through targeting ZEB1 and ZEB2 in GBM. Tumour Biol. 36:6741–6749. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Zhou X, Ren Y, Moore L, Mei M, You Y, Xu P, Wang B, Wang G, Jia Z, Pu P, et al: Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status. Lab Invest. 90:144–155. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Li W, Guo F, Wang P, Hong S and Zhang C: miR-221/222 confers radioresistance in glioblastoma cells through activating Akt independent of PTEN status. Curr Mol Med. 14:185–195. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Xiong X, Deng J, Zeng C, Jiang Y, Tang S and Sun X: MicroRNA-141 is a tumor regulator and prognostic biomarker in human glioblastoma. Oncol Lett. 14:4455–4460. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Avet-Loiseau H, Facon T, Grosbois B, Magrangeas F, Rapp MJ, Harousseau JL, Minvielle S and Bataille R; Intergroupe Francophone du Myélome, : Oncogenesis of multiple myeloma: 14q32 and 13q chromosomal abnormalities are not randomly distributed, but correlate with natural history, immunological features, and clinical presentation. Blood. 99:2185–2191. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Wang ZQ, Zhang MY, Deng ML, Weng NQ, Wang HY and Wu SX: Low serum level of miR-485-3p predicts poor survival in patients with glioblastoma. PLoS One. 12:e01849692017. View Article : Google Scholar : PubMed/NCBI

20 

Tastet J, Decalonne L, Marouillat S, Malvy J, Thépault RA, Toutain A, Paubel A, Tabagh R, Bénédetti H, Laumonnier F, et al: Mutation screening of the ubiquitin ligase gene RNF135 in French patients with autism. Psychiatr Genet. 25:263–267. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Oshiumi H, Matsumoto M, Hatakeyama S and Seya T: Riplet/RNF135, a RING finger protein, ubiquitinates RIG-I to promote interferon-beta induction during the early phase of viral infection. J Biol Chem. 284:807–817. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Jin J, Zhao L and Li Z: The E3 ubiquitin ligase RNF135 regulates the tumorigenesis activity of tongue cancer SCC25 cells. Cancer Med. 5:3140–3146. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Tastet J, Decalonne L, Marouillat S, Malvy J, Thépault RA, Toutain A, Paubel A, Tabagh R, Bénédetti H, Laumonnier F, et al: Mutation screening of the ubiquitin ligase gene RNF135 in French patients with autism. Psychiat Genet. 6:263–267. 2015. View Article : Google Scholar

24 

Liu Y, Wang F, Liu Y, Yao Y, Lv X, Dong B, Li J, Ren S, Yao Y and Xu Y: RNF135, RING finger protein, promotes the proliferation of human glioblastoma cells in vivo and in vitro via the ERK pathway. Sci Rep. 6:206422016. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Lou C, Xiao M, Cheng S, Lu X, Jia S, Ren Y and Li Z: MiR-485-3p and miR-485-5p suppress breast cancer cell metastasis by inhibiting PGC-1α expression. Cell Death Dis. 7:e21592016. View Article : Google Scholar : PubMed/NCBI

27 

Xiong D, Sheng Y, Ding S, Chen J, Tan X, Zeng T, Qin D, Zhu L, Huang A and Tang H: LINC00052 regulates the expression of NTRK3 by miR-128 and miR-485-3p to strengthen HCC cells invasion and migration. Oncotarget. 7:47593–47608. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Batash R, Asna N, Schaffer P, Francis N and Schaffer M: Glioblastoma multiforme, diagnosis and treatment; recent literature review. Curr Med Chem. 24:3002–3009. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Ahir BK, Ozer H, Engelhard HH and Lakka SS: MicroRNAs in glioblastoma pathogenesis and therapy: A comprehensive review. Crit Rev Oncol Hematol. 120:22–33. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Formosa A, Markert EK, Lena AM, Italiano D, Finazzi-Agro' E, Levine AJ, Bernardini S, Garabadgiu AV, Melino G and Candi E: MicroRNAs, miR-154, miR-299-5p, miR-376a, miR-376c, miR-377, miR-381, miR-487b, miR-485-3p, miR-495 and miR-654-3p, mapped to the 14q32.31 locus, regulate proliferation, apoptosis, migration and invasion in metastatic prostate cancer cells. Oncogene. 33:5173–5182. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Henriksen M, Johnsen KB, Olesen P, Pilgaard L and Duroux M: MicroRNA expression signatures and their correlation with clinicopathological features in glioblastoma multiforme. Neuromolecular Med. 16:565–577. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Sun Y: Targeting E3 ubiquitin ligases for cancer therapy. Cancer Biol Ther. 2:623–629. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Xu T, Wang H, Jiang M, Yan Y, Li W, Xu H, Huang Q, Lu Y and Chen J: The E3 ubiquitin ligase CHIP/miR-92b/PTEN regulatory network contributes to tumorigenesis of glioblastoma. Am J Cancer Res. 7:289–300. 2017.PubMed/NCBI

34 

Qi Z, Cai S, Cai J, Chen L, Yao Y, Chen L and Mao Y: miR-491 regulates glioma cells proliferation by targeting TRIM28 in vitro. BMC Neurol. 16:2482016. View Article : Google Scholar : PubMed/NCBI

35 

Douglas J, Cilliers D, Coleman K, Tatton-Brown K, Barker K, Bernhard B, Burn J, Huson S, Josifova D, Lacombe D, et al: Mutations in RNF135, a gene within the NF1 microdeletion region, cause phenotypic abnormalities including overgrowth. Nat Genet. 39:963–965. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Sui R, Chen Y, Liang H, Shi J and Piao H: Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135. Exp Ther Med 18: 475-482, 2019.
APA
Zhang, Y., Sui, R., Chen, Y., Liang, H., Shi, J., & Piao, H. (2019). Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135. Experimental and Therapeutic Medicine, 18, 475-482. https://doi.org/10.3892/etm.2019.7600
MLA
Zhang, Y., Sui, R., Chen, Y., Liang, H., Shi, J., Piao, H."Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135". Experimental and Therapeutic Medicine 18.1 (2019): 475-482.
Chicago
Zhang, Y., Sui, R., Chen, Y., Liang, H., Shi, J., Piao, H."Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135". Experimental and Therapeutic Medicine 18, no. 1 (2019): 475-482. https://doi.org/10.3892/etm.2019.7600
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Sui R, Chen Y, Liang H, Shi J and Piao H: Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135. Exp Ther Med 18: 475-482, 2019.
APA
Zhang, Y., Sui, R., Chen, Y., Liang, H., Shi, J., & Piao, H. (2019). Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135. Experimental and Therapeutic Medicine, 18, 475-482. https://doi.org/10.3892/etm.2019.7600
MLA
Zhang, Y., Sui, R., Chen, Y., Liang, H., Shi, J., Piao, H."Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135". Experimental and Therapeutic Medicine 18.1 (2019): 475-482.
Chicago
Zhang, Y., Sui, R., Chen, Y., Liang, H., Shi, J., Piao, H."Downregulation of miR‑485‑3p promotes glioblastoma cell proliferation and migration via targeting RNF135". Experimental and Therapeutic Medicine 18, no. 1 (2019): 475-482. https://doi.org/10.3892/etm.2019.7600
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