Calcified decellularized arterial scaffolds impact vascular smooth muscle cell transformation via downregulating α‑SMA expression and upregulating OPN expression

Xin,Huaping; Wang,Zhimin; Wu,Shuwu; Wang,Peng; Tao,Xiaoxiao; Xu,Chenhua; You,Liling

doi:10.3892/etm.2019.7626

Calcified decellularized arterial scaffolds impact vascular smooth muscle cell transformation via downregulating α‑SMA expression and upregulating OPN expression

Authors:
- Huaping Xin
- Zhimin Wang
- Shuwu Wu
- Peng Wang
- Xiaoxiao Tao
- Chenhua Xu
- Liling You
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Affiliations: Department of Geriatrics, The People's Hospital of Yichun City, Yichun, Jiangxi 336000, P.R. China, Department of Neurology, Taizhou First People's Hospital, Taizhou, Zhejiang 318000, P.R. China
Published online on: May 29, 2019 https://doi.org/10.3892/etm.2019.7626
Pages: 705-710

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Abstract

The underlying mechanisms of arterial remodeling (AR) remain unclear. Studies have indicated that decellularized scaffolds stimulate the differentiation of fibroblasts into myofibroblasts and promote the accumulation of the extracellular matrix (ECM). In the present study, the impact of ECM changes following AR on vascular smooth muscle cell (VSMC) phenotypes was investigated. VSMCs were co‑cultured with normal or calcified decellularized arterial scaffolds. The expression levels of α‑smooth muscle actin (α‑SMA) and osteopontin (OPN) were measured at 2, 5, 10, 15 and 21 days following the establishment of the co‑culture systems. The expression of α‑SMA in the normal co‑culture group was significantly increased compared with that in the calcified arterial decellularized scaffold co‑culture group (P<0.05 and P<0.001). In addition, the expression of OPN in the AR co‑culture group was significantly increased compared with the normal co‑culture group (P<0.05 and P<0.001). To conclude, the calcified decellularized arterial scaffolds impact VSMC transformation by downregulating α‑SMA expression and upregulating OPN expression (P<0.001). To the best of our knowledge, the present study is the first study that co‑cultured VSMCs with normal or calcified decellularized arterial scaffolds.

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