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Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets

  • Authors:
    • Su Xu
    • Zhao‑Xiu Xu
    • Shuai Yan
    • Jin Le
    • Hao Chen
    • Lan Ming
    • Shu‑Guang Xu
    • Tao Lin
  • View Affiliations / Copyright

    Affiliations: Department of Anorectal Surgery, Yancheng Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Yancheng, Jiangsu 224001, P.R. China, Department of Colorectal Surgery, Kongjiang Hospital of Yangpu, Shanghai 200433, P.R. China, Department of Anorectal Surgery, Suzhou Hospital of Traditional Chinese Medicine, Suzhou, Jiangsu 215009, P.R. China, Department of Anorectal Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1099-1106
    |
    Published online on: June 11, 2019
       https://doi.org/10.3892/etm.2019.7662
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Abstract

The present study investigated the effects and mechanism by which curcumin suppresses intestinal microvascular endothelial cells (INMECs) invasion and angiogenesis induced by activated platelets. INMECs were obtained from healthy rats, and divided into five groups: Control, platelets, platelets +2.5 µM curcumin, platelets +5.0 µM curcumin and platelets +10.0 µM curcumin. Curcumin toxicity was determined and vascular endothelial growth factor (VEGF) concentrations of the five groups were measured using ELISA. The branch point numbers were measured using a capillary tube formation experiment, invasion cell numbers were evaluated with the Transwell assay, relative protein expression levels were measured with western blot assay and immunofluorescence staining of the nucleus. The 2.5, 5 and 10 µM curcumin concentrations were found to be suitable for INMECs. Curcumin significantly downregulated VEGF concentration, suppressed vascular lumen formation and inhibited invasion cell numbers in a dose‑dependent manner. The α‑smooth muscle actin, collagen I, E‑cadherin, phosphorylated (p‑) phosphoinositide 3‑kinase (PI3K), p‑protein kinase B (AKT), p‑mammalian target of rapamycin (m‑TOR) and hypoxia inducible factor subunit alpha (HIF‑1α) protein expression levels of the curcumin‑treated groups were significantly downregulated in a dose‑dependent manner compared with the platelet group. HIF‑1α protein expression levels in the nucleus of the curcumin‑treated groups were significantly suppressed in a dose‑dependent manner compared with the platelet group. In conclusion, curcumin suppressed INMEC invasion and angiogenesis induced by activated platelets via inhibiting the activation of the PI3K/AKT/mTOR pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Xu S, Xu ZX, Yan S, Le J, Chen H, Ming L, Xu SG and Lin T: Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets. Exp Ther Med 18: 1099-1106, 2019.
APA
Xu, S., Xu, Z., Yan, S., Le, J., Chen, H., Ming, L. ... Lin, T. (2019). Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets. Experimental and Therapeutic Medicine, 18, 1099-1106. https://doi.org/10.3892/etm.2019.7662
MLA
Xu, S., Xu, Z., Yan, S., Le, J., Chen, H., Ming, L., Xu, S., Lin, T."Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets". Experimental and Therapeutic Medicine 18.2 (2019): 1099-1106.
Chicago
Xu, S., Xu, Z., Yan, S., Le, J., Chen, H., Ming, L., Xu, S., Lin, T."Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1099-1106. https://doi.org/10.3892/etm.2019.7662
Copy and paste a formatted citation
x
Spandidos Publications style
Xu S, Xu ZX, Yan S, Le J, Chen H, Ming L, Xu SG and Lin T: Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets. Exp Ther Med 18: 1099-1106, 2019.
APA
Xu, S., Xu, Z., Yan, S., Le, J., Chen, H., Ming, L. ... Lin, T. (2019). Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets. Experimental and Therapeutic Medicine, 18, 1099-1106. https://doi.org/10.3892/etm.2019.7662
MLA
Xu, S., Xu, Z., Yan, S., Le, J., Chen, H., Ming, L., Xu, S., Lin, T."Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets". Experimental and Therapeutic Medicine 18.2 (2019): 1099-1106.
Chicago
Xu, S., Xu, Z., Yan, S., Le, J., Chen, H., Ming, L., Xu, S., Lin, T."Curcumin suppresses intestinal microvascular endothelial cells invasion and angiogenesis induced by activated platelets". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1099-1106. https://doi.org/10.3892/etm.2019.7662
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