Effect of mild hypothermia on the expression of IL-10 and IL-18 in neonates with hypoxic ischemic encephalopathy

Wu,Hongwei; Liu,Gang; Yang,Xia; Liu,Qing; Li,Zhenguang

doi:10.3892/etm.2019.7768

Effect of mild hypothermia on the expression of IL-10 and IL-18 in neonates with hypoxic ischemic encephalopathy

Authors:
- Hongwei Wu
- Gang Liu
- Xia Yang
- Qing Liu
- Zhenguang Li
View Affiliations

Affiliations: Department of Newborn Medicine, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, Jiangsu 221006, P.R. China
Published online on: July 12, 2019 https://doi.org/10.3892/etm.2019.7768
Pages: 2194-2198
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The effect of mild hypothermia on the expression of interleukin (IL)-10 and IL-18 in neonates with hypoxic ischemic encephalopathy was investigated. A retrospective analysis was performed on 97 neonates with hypoxic-ischemic encephalopathy (HIE) admitted to the neonatal intensive care unit of Xuzhou Children's Hospital, Xuzhou Medical University from September 2016 to August 2018. Of these patients, 54 with mild hypothermia were included in the experimental group (moderate + severe), and 43 patients who received conventional therapy were included in the control group (moderate + severe). The effect of mild hypothermia on IL-10 and IL-18 expression levels was analyzed, and the relationship between IL-10, IL-18 and disease severity in children with hypoxic ischemic encephalopathy was analyzed. The concentration of IL-10 in the experimental group was significantly lower than that in the control group at 1, 2, 3 and 5 days (P<0.05). The concentration of IL-18 in the experimental group was significantly lower than that in the control group at the 2, 3 and 5 days (P<0.05). NBNA scores of the experimental group were significantly higher than those of the control group at 14 and 28 days after birth (P<0.05). Before treatment, the expression levels of IL-10 and IL-18 in severe children were significantly higher than those in moderate children (P<0.05). IL-10 was positively correlated with the severity of the disease (r=0.521, P<0.05), and IL-18 was also positively correlated with the severity of the disease (r=0.616 P<0.05). Mild hypothermia treatment can effectively improve the neurological function of children with HIE, reduce the expression of IL-10 and IL-18 in serum, and inhibit the inflammatory response. Therefore, the expression of IL-10 and IL-18 was positively correlated with the severity of disease in children with HIE, and could be used as an indicator to judge the severity of HIE.

View Figures

View References

1	Zalewska T, Jaworska J and Ziemka-Nalecz M: Current and experimental pharmacological approaches in neonatal hypoxic- ischemic encephalopathy. Curr Pharm Des. 21:1433–1439. 2015. View Article : Google Scholar : PubMed/NCBI
2	Wang KY, Singer HS, Crain B, Gujar S and Lin DD: Hypoxic-ischemic encephalopathy mimicking acute necrotizing encephalopathy. Pediatr Neurol. 52:110–114. 2015. View Article : Google Scholar : PubMed/NCBI
3	Nash KB, Bonifacio SL, Glass HC, Sullivan JE, Barkovich AJ, Ferriero DM and Cilio MR: Video-EEG monitoring in newborns with hypoxic-ischemic encephalopathy treated with hypothermia. Neurology. 76:556–562. 2011. View Article : Google Scholar : PubMed/NCBI
4	International Liaison Committee on Resuscitation, . 2005 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science with Treatment Recommendations. Part 2: Adult basic life support. Resuscitation. 67:187–201. 2005.PubMed/NCBI
5	Laptook AR, Shankaran S, Tyson JE, Munoz B, Bell EF, Goldberg RN, Parikh NA, Ambalavanan N, Pedroza C, Pappas A, et al Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network, : Effect of therapeutic hypothermia initiated after 6 hours of age on death or disability among newborns with hypoxic-ischemic encephalopathy: A randomized clinical trial. JAMA. 318:1550–1560. 2017. View Article : Google Scholar : PubMed/NCBI
6	Lai JCY, Rocha-Ferreira E, Ek CJ, Wang X, Hagberg H and Mallard C: Immune responses in perinatal brain injury. Brain Behav Immun. 63:210–223. 2017. View Article : Google Scholar : PubMed/NCBI
7	Li SJ, Liu W, Wang JL, Zhang Y, Zhao DJ, Wang TJ and Li YY: The role of TNF-α, IL-6, IL-10, and GDNF in neuronal apoptosis in neonatal rat with hypoxic-ischemic encephalopathy. Eur Rev Med Pharmacol Sci. 18:905–909. 2014.PubMed/NCBI
8	Tsutsumi N, Kimura T, Arita K, Ariyoshi M, Ohnishi H, Yamamoto T, Zuo X, Maenaka K, Park EY, Kondo N, et al: The structural basis for receptor recognition of human interleukin-18. Nat Commun. 5:53402014. View Article : Google Scholar : PubMed/NCBI
9	Galasso JM, Liu Y, Szaflarski J, Warren JS and Silverstein FS: Monocyte chemoattractant protein-1 is a mediator of acute excitotoxic injury in neonatal rat brain. Neuroscience. 101:737–744. 2000. View Article : Google Scholar : PubMed/NCBI
10	Liu L, Zheng CX, Peng SF, Zhou HY, Su ZY, He L and Ai T: Evaluation of urinary S100B protein level and lactate/creatinine ratio for early diagnosis and prognostic prediction of neonatal hypoxic-ischemic encephalopathy. Neonatology. 97:41–44. 2010. View Article : Google Scholar : PubMed/NCBI
11	Nespeca M, Giorgetti C, Nobile S, Ferrini I, Simonato M, Verlato G, Cogo P and Carnielli VP: Does whole-body hypothermia in neonates with hypoxic-ischemic encephalopathy affect surfactant disaturated-phosphatidylcholine kinetics? PLoS One. 11:e01533282016. View Article : Google Scholar : PubMed/NCBI
12	Liao W, Xu H, Ding J and Huang H: Mild hypothermia therapy for moderate or severe hypoxicischemic encephalopathy in neonates. Iran J Public Health. 47:64–69. 2018.PubMed/NCBI
13	Gu Y, He M, Zhou X, Liu J, Hou N, Bin T, Zhang Y, Li T and Chen J: Endogenous IL-6 of mesenchymal stem cell improves behavioral outcome of hypoxic-ischemic brain damage neonatal rats by supressing apoptosis in astrocyte. Sci Rep. 6:185872016. View Article : Google Scholar : PubMed/NCBI
14	Tanaka H, Narita M, Teramoto S, Saikai T, Oashi K, Igarashi T and Abe S: Role of interleukin-18 and T-helper type 1 cytokines in the development of Mycoplasma pneumoniae pneumonia in adults. Chest. 121:1493–1497. 2002. View Article : Google Scholar : PubMed/NCBI
15	Hedtjärn M, Leverin AL, Eriksson K, Blomgren K, Mallard C and Hagberg H: Interleukin-18 involvement in hypoxic-ischemic brain injury. J Neurosci. 22:5910–5919. 2002. View Article : Google Scholar : PubMed/NCBI
16	Liu CQ, Xia YF, Yuan YX, Li L and Qiu XL: Effects of selective head cooling with mild hypothermia on serum levels of caspase-3 and IL-18 in neonates with hypoxic-ischemic encephalopathy. Zhongguo Dang Dai Er Ke Za Zhi. 12:690–692. 2010.(In Chinese). PubMed/NCBI
17	Youn YA, Kim SJ, Sung IK, Chung SY, Kim YH and Lee IG: Serial examination of serum IL-8, IL-10 and IL-1Ra levels is significant in neonatal seizures induced by hypoxic-ischaemic encephalopathy. Scand J Immunol. 76:286–293. 2012. View Article : Google Scholar : PubMed/NCBI
18	Yu XD, Zhang J, Yan CH and Shen XM: Prenatal exposure to manganese at environment relevant level and neonatal neurobehavioral development. Environ Res. 133:232–238. 2014. View Article : Google Scholar : PubMed/NCBI
19	Wu H, Li Z, Yang X, Liu J, Wang W and Liu G: SBDPs and Tau proteins for diagnosis and hypothermia therapy in neonatal hypoxic ischemic encephalopathy. Exp Ther Med. 13:225–229. 2017. View Article : Google Scholar : PubMed/NCBI
20	El-Mazary AA, Nasif KA, Abdel-Hakeem GL, Sherif T, Farouk E and El-Gezawy EM: Adiponectin, leptin and insulin levels at birth and in early postnatal life in neonates with hypoxic ischemic encephalopathy. J Diabetes Metab Disord. 14:872015. View Article : Google Scholar : PubMed/NCBI