MicroRNA‑98‑5p prevents bone regeneration by targeting high mobility group AT‑Hook 2

Zheng,Feng; Wang,Furong; Xu,Zhe

doi:10.3892/etm.2019.7835

MicroRNA‑98‑5p prevents bone regeneration by targeting high mobility group AT‑Hook 2

Authors:
- Feng Zheng
- Furong Wang
- Zhe Xu
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Affiliations: Department of Orthopedics, Qinghai Provincial People's Hospital, Xining, Qinghai 810007, P.R. China
Published online on: July 30, 2019 https://doi.org/10.3892/etm.2019.7835
Pages: 2660-2666

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Abstract

MicroRNAs (mRNAs or miRs) serve an important role in the regulation of gene expression. In the present study, the role of miR‑98‑5p in bone regeneration was determined. Three osteoblast cell models were established, including primary human stem cells (BMMSC), mouse BMMSC's and MC3T3‑E1 cells. miR‑98‑5p expression was determined using reverse transcription‑quantitative (RT‑q)PCR. Osteoblast markers, including alkaline phosphatase, runt related transcription factor 2 and transcription factor Sp7, were determined using RT‑qPCR and western blot analysis, respectively. Alkaline phosphatase activity was determined in the present study and cell proliferation and apoptosis assays were performed. Furthermore, an association between miR‑98‑5p and high mobility group AT‑Hook 2 (HMGA2) was revealed. This association was determined using TargetScan and a dual luciferase reporter assay. The current study demonstrated that miR‑98‑5p was downregulated during osteogenic differentiation and further demonstrated that HMGA2 may be a direct target of miR‑98‑5p. The results also demonstrated that miR‑98‑5p upregulation significantly inhibited the osteogenic differentiation of MC3T3‑E1 cells, an effect that was reversed by an increased HMGA2 expression. Additionally, the results revealed that miR‑98‑5p upregulation inhibited MC3T3‑E1 cell viability and induced cell apoptosis and these effects were eliminated by HMGA2 overexpression. In conclusion, miR‑98‑5p may prevent bone regeneration through inhibiting osteogenic differentiation and osteoblast growth by targeting HMGA2.

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