MicroRNA‑9 inhibits gastric cancer cell proliferation and migration by targeting neuropilin‑1

Hang,Cheng; Yan,Hui‑Shen; Gong,Chen; Gao,Hua; Mao,Qiu‑Hui; Zhu,Jian‑Xin

doi:10.3892/etm.2019.7841

MicroRNA‑9 inhibits gastric cancer cell proliferation and migration by targeting neuropilin‑1

Authors:
- Cheng Hang
- Hui‑Shen Yan
- Chen Gong
- Hua Gao
- Qiu‑Hui Mao
- Jian‑Xin Zhu
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Affiliations: Department of Gastroenterology, Taicang Affiliated Hospital of Soochow University, The First People's Hospital of Taicang, Suzhou, Jiangsu 215400, P.R. China, Department of Traditional Chinese Medicine, Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
Published online on: August 2, 2019 https://doi.org/10.3892/etm.2019.7841
Pages: 2524-2530
Copyright: © Hang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is a global health problem with poor clinical outcomes. The mechanism of its development and progression remains largely unclear. The present study investigated the role of microRNA‑9 (miR‑9‑5p) in the development and progression of GC. Overexpression of miR‑9‑5p led to reduced expression of neuropilin‑1 (NRP‑1) in GC cells. Dual‑luciferase reporter assay results indicated that miR‑9‑5p directly targeted NRP‑1. Furthermore, overexpression of miR‑9‑5p in GC cells increased the expression of mesenchymal markers, N‑cadherin and vimentin, and decreased the expression of epithelial markers, E‑cadherin and β‑catenin. Overexpression of miR‑9‑5p inhibited GC cell proliferation, migration and invasion, and increased the sensitivity of GC cells to the anti‑cancer drug cisplatin. By contrast, the opposite effects were observed in GC cells following downregulation of miR‑9‑5p. Taken together, the present findings suggested that miR‑9‑5p suppressed NRP‑1 expression and inhibited GC cell proliferation and invasion. In addition, miR‑9‑5p overexpression attenuated GC cell resistance to anti‑cancer drugs, which highlighted the potential of miR‑9‑5p as a target for the treatment of GC.

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