Resveratrol alleviates ischemia/reperfusion injury of diabetic myocardium via inducing autophagy

Qu,Xianfeng; Chen,Xiao; Shi,Qingqing; Wang,Xiaofei; Wang,Dongguo; Yang,Li

doi:10.3892/etm.2019.7846

Resveratrol alleviates ischemia/reperfusion injury of diabetic myocardium via inducing autophagy

Authors:
- Xianfeng Qu
- Xiao Chen
- Qingqing Shi
- Xiaofei Wang
- Dongguo Wang
- Li Yang
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Affiliations: Department of Anesthesiology, Municipal Hospital of Taizhou, Taizhou, Zhejiang 318000, P.R. China, Clinical Laboratory, Municipal Hospital of Taizhou, Taizhou, Zhejiang 318000, P.R. China
Published online on: August 2, 2019 https://doi.org/10.3892/etm.2019.7846
Pages: 2719-2725

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Abstract

The rising incidence and complications of diabetes constitutes a major public health issue. The mortality rate of diabetes‑induced myocardial ischemia/reperfusion (I/R) injury is significantly elevated. Resveratrol (RSV) is a naturally occurring polyphenol considered to be a potent cardioprotective compound. The aim of the present study was to explore the function and molecular mechanism of RSV on diabetes‑induced myocardial I/R injury. Left anterior descending coronary artery ligation was performed to stimulate myocardial I/R injury in streptozotocin‑induced diabetic rats. Heart electrical activity was monitored through an electrocardiogram to confirm successful models. The myocardial infarct volume was detected via 2,3,5‑triphenyltetrazolium chloride staining. Western blotting was employed to examine the levels of autophagy markers. It was found that the injection of RSV mitigated the ischemia‑ or I/R injury‑induced myocardial damage on hemodynamic function and infarct size, but the autophagy inhibitor 3‑methyladenine significantly blocked the function of RSV. Furthermore, the application of RSV significantly enhanced the expression of Beclin‑1 and LC‑3II but inhibited the serum levels of tumor necrosis factor‑α and interleukin‑6. These findings revealed an alleviating effect of RSV on diabetes‑induced myocardial I/R injury and provided new evidence for the successful application of RSV on the diabetic myocardium.

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