Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

Ouyang,Hui; Zhang,Li; Xie,Zhen; Ma,Simin

doi:10.3892/etm.2019.7850

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

Authors:
- Hui Ouyang
- Li Zhang
- Zhen Xie
- Simin Ma
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Affiliations: Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Neurology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Nosocomial Infection Management, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
Published online on: August 5, 2019 https://doi.org/10.3892/etm.2019.7850
Pages: 2547-2553
Copyright: © Ouyang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long noncoding RNAs (lncRNAs) have been shown to participate in the development and progression of a number of different types of cancer, including hepatocellular carcinoma (HCC). A recent report has indicated that lncRNA MAFG‑antisense 1 (AS1) promotes colorectal cancer. However, the role of MAFG‑AS1 in other types of cancer remains unclear. The aim of the present study was to examine the effect of lncRNA MAFG‑AS1 in HCC. Based on The Cancer Genome Atlas database and reverse transcription‑quantitative PCR results, it was determined that lncRNA MAFG‑AS1 expression was increased in HCC tissues and cell lines. Following knockdown of lncRNA MAFG‑AS1, a Cell Counting Kit‑8 assay and Transwell assay demonstrated that the proliferation, migration and invasion of HCC cell lines were significantly inhibited. It was additionally demonstrated that there was a negative regulatory association between lncRNA MAFG‑AS1 and miR‑6852. Inhibition of miR‑6852 increased proliferation, migration and invasion of HCC cell lines. LncRNA MAFG‑AS1 promoted HCC development by dampening miR‑6852 function and may thus be a novel target for treating patients with HCC.

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