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Article Open Access

Knockdown of lncRNA CCEPR suppresses colorectal cancer progression

  • Authors:
    • Yuxia Duan
    • Zhixue Fang
    • Zeya Shi
    • Ling Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Hunan Provincial People's Hospital, Changsha, Hunan 410005, P.R. China
    Copyright: © Duan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3534-3542
    |
    Published online on: August 26, 2019
       https://doi.org/10.3892/etm.2019.7942
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Abstract

Long non‑coding RNAs (lncRNAs) serve important roles in colorectal cancer. The aim of the present study was to investigate the expression and role of cervical carcinoma expressed PCNA regulatory (CCEPR) lncRNA in colorectal cancer progression. The results demonstrated that CCEPR expression was significantly higher in colorectal cancer tissues when compared with paired adjacent normal tissues. In addition, CCEPR expression was significantly higher in patients with advanced colorectal cancer (stage III/IV) than those with early‑stage colorectal cancer (stage I/II). High CCEPR expression was significantly associated with poor differentiation, advanced clinical stage, positive lymph node metastasis and distant metastasis. Of particular note, patients with colorectal cancer exhibiting high CCEPR expression levels had shorter survival rates when compared with patients with low CCEPR expression. In vitro experiments demonstrated that the expression of CCEPR was increased in colorectal cancer cell lines when compared with a normal colon cell line. Knockdown of CCEPR significantly inhibited colorectal cancer cell proliferation, colony formation and cell cycle progression, as well as cell migration and invasion. Finally, silencing of CCEPR downregulated matrix metalloproteinase (MMP)‑2 and MMP‑9 expression and suppressed epithelial‑mesenchymal transition in colorectal cancer cells. In conclusion, the results of the present study suggest that CCEPR may exert an oncogenic role in colorectal cancer, and CCEPR may be a promising molecular target for colorectal cancer treatment.
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1 

Cusimano A, Balasus D, Azzolina A, Augello G, Emma MR, Di Sano C, Gramignoli R, Strom SC, McCubrey JA, Montalto G and Cervello M: Oleocanthal exerts antitumor effects on human liver and colon cancer cells through ROS generation. Int J Oncol. 51:533–544. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Munera JO, Sundaram N, Rankin SA, Hill D, Watson C, Mahe M, Vallance JE, Shroyer NF, Sinagoga KL, Zarzoso-Lacoste A, et al: Differentiation of human pluripotent stem cells into colonic organoids via transient activation of BMP signaling. Cell Stem Cell. 24:8292019. View Article : Google Scholar : PubMed/NCBI

5 

Xiao Z, Qu Z, Chen Z, Fang Z, Zhou K, Huang Z, Guo X and Zhang Y: LncRNA HOTAIR is a prognostic biomarker for the proliferation and chemoresistance of colorectal Cancer via MiR-203a-3p-Mediated Wnt/ss-catenin signaling pathway. Cell Physiol Biochem. 46:1275–1285. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Xie B, Deng Z, Pan Y, Fu C, Fan S, Tao Y, Zhou J and Xiao D: Post-transcriptional regulation DPC4 gene by miR-190 in colorectal cancer cells. J Cancer Res Ther. 14:838–843. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Yu SY, Dong B, Tang L and Zhou SH: LncRNA MALAT1 sponges miR-133 to promote NLRP3 inflammasome expression in ischemia-reperfusion injured heart. Int J Cardiol. 254:502018. View Article : Google Scholar : PubMed/NCBI

8 

Xu R, Zhu X, Chen F, Huang C, Ai K, Wu H, Zhang L and Zhao X: LncRNA XIST/miR-200c regulates the stemness properties and tumourigenicity of human bladder cancer stem cell-like cells. Cancer Cell Int. 18:412018. View Article : Google Scholar : PubMed/NCBI

9 

Xiong W, Huang C, Deng H, Jian C, Zen C, Ye K, Zhong Z, Zhao X and Zhu L: Oncogenic non-coding RNA NEAT1 promotes the prostate cancer cell growth through the SRC3/IGF1R/AKT pathway. Int J Biochem Cell Biol. 94:125–132. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Luo J, Chen J, Li H, Yang Y, Yun H, Yang S and Mao X: LncRNA UCA1 promotes the invasion and EMT of bladder cancer cells by regulating the miR-143/HMGB1 pathway. Oncol Lett. 14:5556–5562. 2017.PubMed/NCBI

11 

Zhu H, Zheng T, Yu J, Zhou L and Wang L: LncRNA XIST accelerates cervical cancer progression via upregulating Fus through competitively binding with miR-200a. Biomed Pharmacother. 105:789–797. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Zhang H and Lu W: LncRNA SNHG12 regulates gastric cancer progression by acting as a molecular sponge of miR320. Mol Med Rep. 17:2743–2749. 2018.PubMed/NCBI

13 

Liu K, Yao H, Wen Y, Zhao H, Zhou N, Lei S and Xiong L: Functional role of a long non-coding RNA LIFR-AS1/miR-29a/TNFAIP3 axis in colorectal cancer resistance to pohotodynamic therapy. Biochim Biophys Acta. 1864:2871–2880. 2018. View Article : Google Scholar

14 

Chen Y, Wang CX, Sun XX, Wang C, Liu TF and Wang DJ: Long non-coding RNA CCHE1 overexpression predicts a poor prognosis for cervical cancer. Eur Rev Med Pharmacol Sci. 21:479–483. 2017.PubMed/NCBI

15 

Yang M, Zhai X, Xia B, Wang Y and Lou G: Long noncoding RNA CCHE1 promotes cervical cancer cell proliferation via upregulating PCNA. Tumour Biol. 36:7615–7622. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Xu G, Zhang Y, Li N, Zhang JB and Xu R: LncRNA CCHE1 in the proliferation and apoptosis of gastric cancer cells. Eur Rev Med Pharmacol Sci. 22:2631–2637. 2018.PubMed/NCBI

17 

Liao Y, Cheng S, Xiang J and Luo C: lncRNA CCHE1 increased proliferation, metastasis and invasion of non-small lung cancer cells and predicted poor survival in non-small lung cancer patients. Eur Rev Med Pharmacol Sci. 22:1686–1692. 2018.PubMed/NCBI

18 

Peng W and Fan H: Long noncoding RNA CCHE1 indicates a poor prognosis of hepatocellular carcinoma and promotes carcinogenesis via activation of the ERK/MAPK pathway. Biomed Pharmacother. 83:450–455. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Zhan Y, Li Y, Guan B, Chen X, Chen Z, He A, He S, Gong Y, Peng D, Liu Y, et al: Increased expression of long non-coding RNA CCEPR is associated with poor prognosis and promotes tumorigenesis in urothelial bladder carcinoma. Oncotarget. 8:44326–44334. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Gaballah HH, Gaber RA, Elrashidy MA, Elshahat DA, Hablus MA and Ebeid AM: Expression of long non-coding RNA CCHE1 in colorectal carcinoma: Correlations with clinicopathological features and ERK/COX-2 pathway. Mol Biol Rep. 46:657–667. 2019. View Article : Google Scholar : PubMed/NCBI

21 

Pietruszewska W, Bojanowska-Pozniak K and Kobos J: Matrix metalloproteinases MMP1, MMP2, MMP9 and their tissue inhibitors TIMP1, TIMP2, TIMP3 in head and neck cancer: An immunohistochemical study. Otolaryngol Pol. 70:32–43. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Wu Y, Sarkissyan M and Vadgama JV: Epithelial-mesenchymal transition and breast cancer. J Clin Med. 5(pii): E132016. View Article : Google Scholar : PubMed/NCBI

23 

Jiang Z, Song Q, Zeng R, Li J, Li J, Lin X, Chen X, Zhang J and Zheng Y: MicroRNA-218 inhibits EMT, migration and invasion by targeting SFMBT1 and DCUN1D1 in cervical cancer. Oncotarget. 7:45622–45636. 2016.PubMed/NCBI

24 

Barrette K, Van Kelst S, Wouters J, Marasigan V, Fieuws S, Agostinis P, van den Oord J and Garmyn M: Epithelial-mesenchymal transition during invasion of cutaneous squamous cell carcinoma is paralleled by AKT activation. Br J Dermatol. 171:1014–1021. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Yang F, Yu N, Wang H, Zhang C, Zhang Z, Li Y, Li D, Yan L, Liu H and Xu Z: Downregulated expression of hepatoma-derived growth factor inhibits migration and invasion of prostate cancer cells by suppressing epithelial-mesenchymal transition and MMP2, MMP9. PLoS One. 13:e01907252018. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Zhu L, Yang N, Du G, Li C, Liu G, Liu S, Xu Y, Di Y, Pan W and Li X: LncRNA CRNDE promotes the epithelial-mesenchymal transition of hepatocellular carcinoma cells via enhancing the Wnt/β-catenin signaling pathway. J Cell Biochem. 2018.(Epub ahead of prin).

28 

Zhou Y, Chen Y, Ding W, Hua Z, Wang L, Zhu Y, Qian H and Dai T: LncRNA UCA1 impacts cell proliferation, invasion, and migration of pancreatic cancer through regulating miR-96/FOXO3. IUBMB Life. 70:276–290. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Yang Y, Shen Z, Yan Y, Wang B, Zhang J, Shen C, Li T, Ye C, Gao Z, Peng G, et al: Long non-coding RNA GAS5 inhibits cell proliferation, induces G0/G1 arrest and apoptosis, and functions as a prognostic marker in colorectal cancer. Oncol Lett. 13:3151–3158. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Wang JZ, Xu CL, Wu H and Shen SJ: LncRNA SNHG12 promotes cell growth and inhibits cell apoptosis in colorectal cancer cells. Braz J Med Biol Res. 50:e60792017. View Article : Google Scholar : PubMed/NCBI

31 

Smith BN and Bhowmick NA: Role of EMT in metastasis and therapy resistance. J Clin Med. 5(pii): E172016. View Article : Google Scholar : PubMed/NCBI

32 

Chen DL, Chen LZ, Lu YX, Zhang DS, Zeng ZL, Pan ZZ, Huang P, Wang FH, Li YH, Ju HQ and Xu RH: Long noncoding RNA XIST expedites metastasis and modulates epithelial-mesenchymal transition in colorectal cancer. Cell Death Dis. 8:e30112017. View Article : Google Scholar : PubMed/NCBI

33 

Tao Y, Han T, Zhang T, Ma C and Sun C: LncRNA CHRF-induced miR-489 loss promotes metastasis of colorectal cancer via TWIST1/EMT signaling pathway. Oncotarget. 8:36410–36422. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Yang X, Liu W, Xu X, Zhu J, Wu Y, Zhao K, He S, Li M, Wu Y, Zhang S, et al: Downregulation of long noncoding RNA UCA1 enhances the radiosensitivity and inhibits migration via suppression of epithelialmesenchymal transition in colorectal cancer cells. Oncol Rep. 40:1554–1564. 2018.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Duan Y, Fang Z, Shi Z and Zhang L: Knockdown of lncRNA CCEPR suppresses colorectal cancer progression. Exp Ther Med 18: 3534-3542, 2019.
APA
Duan, Y., Fang, Z., Shi, Z., & Zhang, L. (2019). Knockdown of lncRNA CCEPR suppresses colorectal cancer progression. Experimental and Therapeutic Medicine, 18, 3534-3542. https://doi.org/10.3892/etm.2019.7942
MLA
Duan, Y., Fang, Z., Shi, Z., Zhang, L."Knockdown of lncRNA CCEPR suppresses colorectal cancer progression". Experimental and Therapeutic Medicine 18.5 (2019): 3534-3542.
Chicago
Duan, Y., Fang, Z., Shi, Z., Zhang, L."Knockdown of lncRNA CCEPR suppresses colorectal cancer progression". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3534-3542. https://doi.org/10.3892/etm.2019.7942
Copy and paste a formatted citation
x
Spandidos Publications style
Duan Y, Fang Z, Shi Z and Zhang L: Knockdown of lncRNA CCEPR suppresses colorectal cancer progression. Exp Ther Med 18: 3534-3542, 2019.
APA
Duan, Y., Fang, Z., Shi, Z., & Zhang, L. (2019). Knockdown of lncRNA CCEPR suppresses colorectal cancer progression. Experimental and Therapeutic Medicine, 18, 3534-3542. https://doi.org/10.3892/etm.2019.7942
MLA
Duan, Y., Fang, Z., Shi, Z., Zhang, L."Knockdown of lncRNA CCEPR suppresses colorectal cancer progression". Experimental and Therapeutic Medicine 18.5 (2019): 3534-3542.
Chicago
Duan, Y., Fang, Z., Shi, Z., Zhang, L."Knockdown of lncRNA CCEPR suppresses colorectal cancer progression". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3534-3542. https://doi.org/10.3892/etm.2019.7942
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