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MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma

  • Authors:
    • Changfu Liu
    • Zhou Tong
    • Jingyu Tan
    • Zengxi Xin
    • Zhiying Wang
    • Luming Tian
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Stomatology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Prosthodontics, The Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Dental Implants, The Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3543-3551
    |
    Published online on: September 2, 2019
       https://doi.org/10.3892/etm.2019.7970
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Abstract

The aim of the present study was to analyze the role of microRNA (miRNA)‑21‑5p in tongue squamous cell carcinoma (TSCC), predict the target gene of miR‑21‑5p and provide novel strategies for gene therapy in TSCC treatment. The expression levels of miRNA‑21‑5p in TSCC tissues were analyzed using reverse transcription quantitative polymerase chain reaction, and the effects of miRNA‑21‑5p on cell proliferation, invasion and apoptosis and the expression levels of target protein PDCD4 in the Cal 27 and SCC9 cell lines were determined. PI3K/AKT/Forkhead Box O1 (FOXO1) pathway‑associated protein expression levels were evaluated by western blot analysis. miRNA‑21‑5p was consistently upregulated in TSCC tissues compared with normal tissues. Inhibition of miR‑21‑5p inhibited cell proliferation and invasion, and promoted cell apoptosis. A luciferase reporter assay confirmed that PDCD4 was the target of miR‑21‑5p. Inhibition of miRNA21‑5p suppressed the PI3K/Akt/FOXO1 signaling pathway. The results from the present study indicated that miR‑21‑5p‑targeting PDCD4 suppresses apoptosis in human TSCC cell lines. This anti‑apoptotic effect was achieved by regulating the PI3K/Akt/FOXO1 signaling pathway. These data represent the basis for a promising novel strategy for the treatment of TSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Tong Z, Tan J, Xin Z, Wang Z and Tian L: MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma. Exp Ther Med 18: 3543-3551, 2019.
APA
Liu, C., Tong, Z., Tan, J., Xin, Z., Wang, Z., & Tian, L. (2019). MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma. Experimental and Therapeutic Medicine, 18, 3543-3551. https://doi.org/10.3892/etm.2019.7970
MLA
Liu, C., Tong, Z., Tan, J., Xin, Z., Wang, Z., Tian, L."MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma". Experimental and Therapeutic Medicine 18.5 (2019): 3543-3551.
Chicago
Liu, C., Tong, Z., Tan, J., Xin, Z., Wang, Z., Tian, L."MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3543-3551. https://doi.org/10.3892/etm.2019.7970
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Tong Z, Tan J, Xin Z, Wang Z and Tian L: MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma. Exp Ther Med 18: 3543-3551, 2019.
APA
Liu, C., Tong, Z., Tan, J., Xin, Z., Wang, Z., & Tian, L. (2019). MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma. Experimental and Therapeutic Medicine, 18, 3543-3551. https://doi.org/10.3892/etm.2019.7970
MLA
Liu, C., Tong, Z., Tan, J., Xin, Z., Wang, Z., Tian, L."MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma". Experimental and Therapeutic Medicine 18.5 (2019): 3543-3551.
Chicago
Liu, C., Tong, Z., Tan, J., Xin, Z., Wang, Z., Tian, L."MicroRNA‑21‑5p targeting PDCD4 suppresses apoptosis via regulating the PI3K/AKT/FOXO1 signaling pathway in tongue squamous cell carcinoma". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3543-3551. https://doi.org/10.3892/etm.2019.7970
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