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Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9

  • Authors:
    • Shoujie Wang
    • Caiyun Li
    • Yijia Yu
    • Jianjun Qiao
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Dermatology, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3450-3460
    |
    Published online on: September 5, 2019
       https://doi.org/10.3892/etm.2019.7972
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Abstract

The present study aimed to determine the expression of microRNA (miRNA or miR)‑145 in hypertrophic scars at the tissue and cellular levels, and to investigate its biological functions and mechanism of action. A total of 36 patients who were diagnosed with hypertrophic scar were included in the present study. Reverse transcription‑quantitative polymerase chain reaction was used to determine the expression of miR‑145 in tissues and fibroblasts. Primary fibroblasts were transfected with negative control miRNA, miR‑145 mimics or inhibitor. A Cell Counting Kit‑8 assay was performed to determine the level of proliferation of fibroblasts. Flow cytometry was employed for cell cycles determination and apoptosis in fibroblasts. A Matrigel assay was used to evaluate the invasion ability of fibroblasts. Western blotting was used to determine the expression of the transcription factor SOX‑9 (SOX‑9) protein in fibroblasts. Rescue experiments were performed to examine the effect of SOX‑9 on the regulation of fibroblasts by miR‑145. The dual luciferase reporter assay was performed to identify the direct interaction between SOX‑9 and miR‑145. The expression of miR‑145 was reduced in hypertrophic tissues and fibroblasts. Overexpression of miR‑145 inhibited the proliferation, G1/S phase transition and invasion of fibroblasts, and promoted the apoptosis of fibroblasts. In addition, overexpression of miR‑145 inhibited SOX‑9 protein expression. By contrast, the expression of SOX‑9 reversed the effects of miR‑145 on the proliferation, cell cycle, apoptosis and invasion of fibroblasts. The miR‑145 seed region was able to bind with the 3'‑untranslated region of the SOX‑9 mRNA to regulate its expression. The present study demonstrated that miR‑145 expression is reduced in hypertrophic scar tissues and negatively associated with SOX‑9 expression. In addition, miR‑145 inhibits the proliferation, cell cycle and invasion, and promotes the apoptosis of fibroblasts by down‑regulating the expression of SOX‑9. The current study provides a potential target for the clinical diagnosis and treatment of hypertrophic scars.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Li C, Yu Y and Qiao J: Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9. Exp Ther Med 18: 3450-3460, 2019.
APA
Wang, S., Li, C., Yu, Y., & Qiao, J. (2019). Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9. Experimental and Therapeutic Medicine, 18, 3450-3460. https://doi.org/10.3892/etm.2019.7972
MLA
Wang, S., Li, C., Yu, Y., Qiao, J."Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9". Experimental and Therapeutic Medicine 18.5 (2019): 3450-3460.
Chicago
Wang, S., Li, C., Yu, Y., Qiao, J."Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3450-3460. https://doi.org/10.3892/etm.2019.7972
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Li C, Yu Y and Qiao J: Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9. Exp Ther Med 18: 3450-3460, 2019.
APA
Wang, S., Li, C., Yu, Y., & Qiao, J. (2019). Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9. Experimental and Therapeutic Medicine, 18, 3450-3460. https://doi.org/10.3892/etm.2019.7972
MLA
Wang, S., Li, C., Yu, Y., Qiao, J."Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9". Experimental and Therapeutic Medicine 18.5 (2019): 3450-3460.
Chicago
Wang, S., Li, C., Yu, Y., Qiao, J."Decreased expression of microRNA‑145 promotes the biological functions of fibroblasts in hypertrophic scar tissues by upregulating the expression of transcription factor SOX‑9". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3450-3460. https://doi.org/10.3892/etm.2019.7972
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