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MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α

  • Authors:
    • Yan Liu
    • Jianfeng Zou
    • Xiaoyan Liu
    • Quan Zhang
  • View Affiliations / Copyright

    Affiliations: The First Ward, Department of Cardiology, Rizhao People's Hospital, Rizhao, Shandong 276800, P.R. China, The Third Ward, Department of Cardiology, Rizhao People's Hospital, Rizhao, Shandong 276800, P.R. China, Department of Cardiology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 260141, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3325-3332
    |
    Published online on: September 5, 2019
       https://doi.org/10.3892/etm.2019.7976
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Abstract

Myocardial ischemia‑reperfusion (I/R) injury is considered to have a detrimental role in coronary heart disease, which is considered to be the leading cause of death worldwide. However, the molecular mechanism involved in the progression of myocardial I/R injury is still unclear. The current study aimed to investigate the expression and function of microRNA (miR)‑138 in the process of myocardial I/R injury. First, miR‑138 expression levels were analyzed both in myocardium with I/R injury and control myocardium using reverse transcription‑quantitative polymerase chain reaction analysis. Then, the relationship between the levels of miR‑138 and hypoxia‑inducible factor (HIF)1‑α was also investigated using a luciferase reporter assay. Assessment of myocardial infarct size, measurements of serum myocardial enzymes and electron microscopy analysis were all utilized to analyse the effect of miR‑138 on myocardial I/R injury. The authors of current study also used western blotting to examine the expression levels of the mitochondrial fission‑related proteins dynamin‑1‑like protein and mitochondrial fission 1 protein. It was found that miR‑138 is downregulated and HIF1‑α is upregulated after myocardial ischemia reperfusion injury. Overexpression of miR‑138 reduced myocardial I/R injury‑induced infarct sizes and myocardial enzyme levels, and it also inhibited the expression of proteins related to mitochondrial morphology and myocardial I/R‑induced mitochondrial apoptosis by targeting HIF1‑α. Taken together, these findings provide a novel insight into the molecular mechanism of miR‑138 and HIF1‑α in the progression of myocardial I/R injury. miR‑138 has the potential to become a promising therapeutic target for treating myocardial I/R injury.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Zou J, Liu X and Zhang Q: MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α. Exp Ther Med 18: 3325-3332, 2019.
APA
Liu, Y., Zou, J., Liu, X., & Zhang, Q. (2019). MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α. Experimental and Therapeutic Medicine, 18, 3325-3332. https://doi.org/10.3892/etm.2019.7976
MLA
Liu, Y., Zou, J., Liu, X., Zhang, Q."MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α". Experimental and Therapeutic Medicine 18.5 (2019): 3325-3332.
Chicago
Liu, Y., Zou, J., Liu, X., Zhang, Q."MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3325-3332. https://doi.org/10.3892/etm.2019.7976
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Zou J, Liu X and Zhang Q: MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α. Exp Ther Med 18: 3325-3332, 2019.
APA
Liu, Y., Zou, J., Liu, X., & Zhang, Q. (2019). MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α. Experimental and Therapeutic Medicine, 18, 3325-3332. https://doi.org/10.3892/etm.2019.7976
MLA
Liu, Y., Zou, J., Liu, X., Zhang, Q."MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α". Experimental and Therapeutic Medicine 18.5 (2019): 3325-3332.
Chicago
Liu, Y., Zou, J., Liu, X., Zhang, Q."MicroRNA‑138 attenuates myocardial ischemia reperfusion injury through inhibiting mitochondria‑mediated apoptosis by targeting HIF1‑α". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3325-3332. https://doi.org/10.3892/etm.2019.7976
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